EC Number   |
Activating Compound |
Reference |
|---|
   6.5.1.2 | (NH4)2SO4 |
with 30 mM (NH4)2SO4, the activity is maximal |
715672 |
| 6.5.1.2 | bovine serum albumin |
0.025 mg/ml, stimulating |
651073 |
| 6.5.1.2 | dithiothreitol |
10 mM, stimulating |
651073 |
| 6.5.1.2 | KCl |
150 mM |
653379 |
| 6.5.1.2 | KCl |
activation by KCl is observed at (NH4)2SO4 concentrations below 30 mM, in the absence of (NH4)2SO4 ligase activity is activated by KCl over 0-100 mM, and at 100 mM KCl, further activation occurs at up to 12 mM (NH4)2SO4 |
715672 |
| 6.5.1.2 | KCl |
highest activity at 150 mM |
651212 |
| 6.5.1.2 | Macromolecular solutes |
blunt-end ligation in presence of high concentrations of macromolecular solutes, inactive in absence, e.g. polyethylene glycol 6000, bovine plasma albumin, or Ficoll 70 |
1972 |
| 6.5.1.2 | more |
no requirement for sulfhydryl reagent |
1909, 1910 |
| 6.5.1.2 | NaCl |
highest activity at 200 mM |
651212 |
| 6.5.1.2 | NH4Cl |
highest activity at 200 mM |
651212 |
