Activating Compound | Comment | Organism | Structure |
---|---|---|---|
KCl | 150 mM | Aquifex aeolicus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Aquifex aeolicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00008 | - |
nicked DNA | pH 7.6, 65°C | Aquifex aeolicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | supports formation of DNA-adenylate intermediate | Aquifex aeolicus | |
Mg2+ | 5 mM | Aquifex aeolicus | |
Mn2+ | 5 mM, most effective metal cofactor | Aquifex aeolicus | |
Ni2+ | supports formation of DNA-adenylate intermediate | Aquifex aeolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Aquifex aeolicus | DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | O66880 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Aquifex aeolicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide | 3 steps of reaction: 1. adenylation of the ligase in the presence of NAD+, 2. transferring the adenylate moiety to the 5'-phosphate of the nicked DNA substrate, 3. sealing the nick through the formation of a phosphodiester bond | Aquifex aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + nicked DNA | - |
Aquifex aeolicus | AMP + diphosphate + ? | - |
? | |
ATP + nicked DNA | - |
Aquifex aeolicus | ? | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | activity depends on the base pairs on both sides of the nick, T/AAquifex aeolicus |
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m |
- ? |
| | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA | Aquifex aeolicus | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
nicked DNA | pH 7.6, 65°C | Aquifex aeolicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Aquifex aeolicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | required, highest activity at 0.5 mM | Aquifex aeolicus |