Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C78A | almost complete loss of activity | Escherichia coli |
C78S | complete loss of activity | Escherichia coli |
D75A | almost complete loss of activity | Escherichia coli |
D75E | complete loss of activity | Escherichia coli |
D75N | complete loss of activity | Escherichia coli |
H168A | almost complete loss of activity | Escherichia coli |
H168N | complete loss of activity | Escherichia coli |
H168Q | complete loss of activity | Escherichia coli |
H185A | activity is severely impaired | Escherichia coli |
H280A | active, protein is able to complement a Saccharomyces cerevisiae Trl1 mutant | Escherichia coli |
H337A | almost complete loss of activity | Escherichia coli |
H337N | complete loss of activity | Escherichia coli |
H337Q | complete loss of activity | Escherichia coli |
K298A | activity is severely impaired | Escherichia coli |
N167A | activity is severely impaired | Escherichia coli |
R189A | almost complete loss of activity | Escherichia coli |
R189A | activity is severely impaired | Escherichia coli |
R341A | almost complete loss of activity | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required | Escherichia coli | |
additional information | Mg2+ cannot substitute for Mn2+ | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P46850 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O | substrate mimicks the broken tRNAGlu(UUC) anticodon stem-loop generated by Kluyveromyces lactis gamma-toxin, leaving 2',3'-cyclic phosphate and 5'-OH ends | Escherichia coli | (ribonucleotide)n+m + GMP + diphosphate | overall reaction | ? | |
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + GTP | - |
Escherichia coli | (ribonucleotide)n+m + GMP + diphosphate | overall reaction, substrate 5'-hydroxy-(ribonucleotide)m can be composed of all ribonucleotides or all deoxynucleotides | ? | |
(ribonucleotide)n-3'-phosphate + 5'-phospho-(ribonucleotide)m + GTP | - |
Escherichia coli | (ribonucleotide)n+m + GMP + diphosphate | overall reaction | ? |
General Information | Comment | Organism |
---|---|---|
physiological function | RtcB is able in vivo to complement growth of yeast cells that lack the endogenous healing/sealing-type tRNA ligase Trl1. RtcB also protects yeast Trl1 mutant cells against a fungal ribotoxin that incises the anticodon loop of cellular tRNAs. RtcB can replace Trl1 as the catalyst of HAC1 mRNA splicing during the unfolded protein response | Escherichia coli |