Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pantoea agglomerans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.55 | - |
fumarate | pH 8.0, 22°C | Pantoea agglomerans | |
20 | - |
succinate | pH 8.0, 22°C | Pantoea agglomerans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + fumarate + (2S)-2,3-diaminopropionate | Pantoea agglomerans | the enzyme is involved in biosynthesis of dapdiamide antibiotics (a family of fumaramoyl- and epoxysuccinamoyl-dipeptides, named for the presence of 2,3-diaminopropionate (DAP) and the two amide linkages of the antibiotic scaffold) | AMP + diphosphate + (2E)-4-[(2-amino-2-carboxyethyl)amino]-4-oxobut-2-enoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pantoea agglomerans | E2JA32 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pantoea agglomerans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + fumaramate + (2S)-2,3-diaminopropionate | activity with fumaramate is about 15% of the activity compared to fumarate | Pantoea agglomerans | ? | - |
? | |
ATP + fumarate + (2S)-2,3-diaminopropionate | - |
Pantoea agglomerans | AMP + diphosphate + (2E)-4-[(2-amino-2-carboxyethyl)amino]-4-oxobut-2-enoate | - |
? | |
ATP + fumarate + (2S)-2,3-diaminopropionate | the enzyme is involved in biosynthesis of dapdiamide antibiotics (a family of fumaramoyl- and epoxysuccinamoyl-dipeptides, named for the presence of 2,3-diaminopropionate (DAP) and the two amide linkages of the antibiotic scaffold) | Pantoea agglomerans | AMP + diphosphate + (2E)-4-[(2-amino-2-carboxyethyl)amino]-4-oxobut-2-enoate | - |
? | |
ATP + succinate + (2S)-2,3-diaminopropionate | activity with succinate is less than 5% of the activity compared to fumarate | Pantoea agglomerans | ? | - |
? | |
additional information | no activity with the half-amides fumaramate and succinamate succinamate, or with the two enantiomers of trans-epoxysuccinate | Pantoea agglomerans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DdaG | - |
Pantoea agglomerans |
fumaroyl-2,3-diaminopropionate ligase | - |
Pantoea agglomerans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Pantoea agglomerans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.8 | - |
succinate | pH 8.0, 22°C | Pantoea agglomerans | |
11.6 | - |
fumarate | pH 8.0, 22°C | Pantoea agglomerans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pantoea agglomerans |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in biosynthesis of dapdiamide antibiotics (a family of fumaramoyl- and epoxysuccinamoyl-dipeptides, named for the presence of 2,3-diaminopropionate (DAP) and the two amide linkages of the antibiotic scaffold) | Pantoea agglomerans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
succinate | pH 8.0, 22°C | Pantoea agglomerans | |
21 | - |
fumarate | pH 8.0, 22°C | Pantoea agglomerans |