Information on EC 6.3.2.46 - fumarate-(S)-2,3-diaminopropanoate ligase

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The expected taxonomic range for this enzyme is: Pantoea agglomerans

EC NUMBER
COMMENTARY hide
6.3.2.46
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RECOMMENDED NAME
GeneOntology No.
fumarate-(S)-2,3-diaminopropanoate ligase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + fumarate + (S)-2,3-diaminopropanoate = AMP + diphosphate + N3-fumaroyl-(S)-2,3-diaminopropanoate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
dapdiamides biosynthesis
SYSTEMATIC NAME
IUBMB Comments
fumarate:(S)-2,3-diaminopropanoate ligase (AMP-forming)
The enzyme, characterized from the bacterium Enterobacter agglomerans, is involved in biosynthesis of dapdiamide tripeptide antibiotics, a family of fumaramoyl- and epoxysuccinamoyl-peptides named for the presence of an (S)-2,3-diaminopropanoate (DAP) moiety and two amide linkages in their scaffold.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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the enzyme is involved in biosynthesis of dapdiamide antibiotics (a family of fumaramoyl- and epoxysuccinamoyl-dipeptides, named for the presence of 2,3-diaminopropionate (DAP) and the two amide linkages of the antibiotic scaffold)
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + fumaramate + (2S)-2,3-diaminopropionate
?
show the reaction diagram
activity with fumaramate is about 15% of the activity compared to fumarate
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?
ATP + fumarate + (2S)-2,3-diaminopropionate
AMP + diphosphate + (2E)-4-[(2-amino-2-carboxyethyl)amino]-4-oxobut-2-enoate
show the reaction diagram
ATP + succinate + (2S)-2,3-diaminopropionate
?
show the reaction diagram
activity with succinate is less than 5% of the activity compared to fumarate
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?
additional information
?
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no activity with the half-amides fumaramate and succinamate succinamate, or with the two enantiomers of trans-epoxysuccinate
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + fumarate + (2S)-2,3-diaminopropionate
AMP + diphosphate + (2E)-4-[(2-amino-2-carboxyethyl)amino]-4-oxobut-2-enoate
show the reaction diagram
E2JA32
the enzyme is involved in biosynthesis of dapdiamide antibiotics (a family of fumaramoyl- and epoxysuccinamoyl-dipeptides, named for the presence of 2,3-diaminopropionate (DAP) and the two amide linkages of the antibiotic scaffold)
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?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.55
fumarate
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pH 8.0, 22°C
20
succinate
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pH 8.0, 22°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
11.6
fumarate
Pantoea agglomerans
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pH 8.0, 22°C
3.8
succinate
Pantoea agglomerans
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pH 8.0, 22°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
21
fumarate
Pantoea agglomerans
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pH 8.0, 22°C
170
0.19
succinate
Pantoea agglomerans
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pH 8.0, 22°C
58
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
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assay at
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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