Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.5.1.18 extracted from

  • Cunningham, F.X. Jr.; Gantt, E.
    One ring or two? Determination of ring number in carotenoids by lycopene epsilon-cyclases (2001), Proc. Natl. Acad. Sci. USA, 98, 2905-2910.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.5.1.18

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Lactuca sativa
expression in Escherichia coli Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
A447S like the wild-type enzyme the mutant enzyme adds two epsilon-rings to lycopene, forming epsilon-carotene Arabidopsis thaliana
H457D the mutant enzyme forms a higher amount of ratio delta-carotene compared to the wild-type enzyme Lactuca sativa
H457L the mutant adds only one epsilon-ring to lycopene, forming delta-carotene Lactuca sativa
H457R like the wild-type enzyme the mutant enzyme predominantly form epsilon-carotene (91%) Lactuca sativa
L448H the mutant adds two epsilon-ring to lycopene, forming epsilon-carotene Arabidopsis thaliana
L448R the mutant adds two epsilon-ring to lycopene, forming epsilon-carotene Arabidopsis thaliana
additional information construction and characterization of chimeric epsilon-cyclases of the epsilion-cyclases from Lactuca sativa and Arabidopsis thaliana Lactuca sativa
additional information construction and characterization of chimeric epsilon-cyclases of the epsilion-cyclases from Lactuca sativa and Arabidopsis thaliana Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q38932
-
-
Lactuca sativa Q9AXK7
-
-
Lactuca sativa var. romaine Q9AXK7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lycopene wild-type enzyme and mutant enzyme A447D acts one psi end of lycopene and forms monocyclic delta-carotene. Mutant enzymes L448H and L448R add two epsilon-ring to lycopene, forming epsilon-carotene Arabidopsis thaliana delta-carotene
-
 ?
lycopene the enzyme acts on both psi ends of lycopene, forming bicyclic epsilon-carotene. Mutant enzyme H457L adds only one epsilon-ring to lycopene, forming delta-carotene Lactuca sativa epsilon-carotene
-
 ?
lycopene the enzyme acts on both psi ends of lycopene, forming bicyclic epsilon-carotene. Mutant enzyme H457L adds only one epsilon-ring to lycopene, forming delta-carotene Lactuca sativa var. romaine epsilon-carotene
-
 ?

Synonyms

Synonyms Comment Organism
LCYe
-
 Lactuca sativa
LCYe
-
 Arabidopsis thaliana
lycopene bi-epsilon-cyclase
-
 Lactuca sativa