Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
delta-carotene
epsilon-carotene
-
-
-
?
gamma-carotene
alpha-carotene
lycopene
delta-carotene + beta-carotene + alpha-carotene + epsilon-carotene
lycopene
epsilon-carotene
additional information
?
-
gamma-carotene
alpha-carotene
-
-
-
?
gamma-carotene
alpha-carotene
-
-
-
-
?
lycopene
delta-carotene
-
-
-
?
lycopene
delta-carotene
the cyclization of lycopene (psi,psi-carotene) is a key branch point in the pathway of carotenoid biosynthesis
-
-
?
lycopene
delta-carotene
wild-type enzyme and mutant enzyme A447D acts one psi end of lycopene and forms monocyclic delta-carotene. Mutant enzymes L448H and L448R add two epsilon-ring to lycopene, forming epsilon-carotene
-
-
?
lycopene
delta-carotene
-
-
-
?
lycopene
delta-carotene
-
-
-
?
lycopene
delta-carotene
-
-
-
?
lycopene
delta-carotene
lycopene epsilon-cyclase adds one epsilon-ring to synthesize delta-carotene, (6R)-epsilon,psi-carotene
-
-
?
lycopene
delta-carotene
-
-
-
-
?
lycopene
delta-carotene
-
-
-
-
?
lycopene
delta-carotene
-
-
-
-
?
lycopene
delta-carotene
-
-
-
-
?
lycopene
delta-carotene + beta-carotene + alpha-carotene + epsilon-carotene
the enzyme OsLCYe catalyzes the formation of beta-carotene or delta-carotene from lycopene, and as side activities the formation of gamma-, alpha-, or epsilon-carotene from lycopene, reactions catalyzed by OsLCYe, overview
-
-
?
lycopene
delta-carotene + beta-carotene + alpha-carotene + epsilon-carotene
the enzyme OsLCYe catalyzes the formation of beta-carotene or delta-carotene from lycopene, and as side actities the formation of gamma-, alpha-, or epsilon-carotene from lycopene, reactions catalyzed by OsLCYe, overview
-
-
?
lycopene
epsilon-carotene
the enzyme acts on both psi ends of lycopene, forming bicyclic epsilon-carotene. Mutant enzyme H457L adds only one epsilon-ring to lycopene, forming delta-carotene
-
-
?
lycopene
epsilon-carotene
the enzyme acts on both psi ends of lycopene, forming bicyclic epsilon-carotene. Mutant enzyme H457L adds only one epsilon-ring to lycopene, forming delta-carotene
-
-
?
lycopene
epsilon-carotene
-
-
-
-
?
lycopene
epsilon-carotene
-
bifunctional enzyme catalyzing the formation of epsilon- as well as of beta-ionone end groups
-
-
?
lycopene
epsilon-carotene
-
-
-
-
?
lycopene
epsilon-carotene
-
bifunctional enzyme catalyzing the formation of epsilon- as well as of beta-ionone end groups
-
-
?
additional information
?
-
5-cis-lycopene is not involved in the reaction with OsLCYe acting as an exclusion filter for this naturally occurring isomer. Using liposomes containing 5-cis-lycopene, OsLCYe produces the same moncyclic gamma/delts-carotene pattern as with all-trans-lycopene
-
-
?
additional information
?
-
-
5-cis-lycopene is not involved in the reaction with OsLCYe acting as an exclusion filter for this naturally occurring isomer. Using liposomes containing 5-cis-lycopene, OsLCYe produces the same moncyclic gamma/delts-carotene pattern as with all-trans-lycopene
-
-
?
additional information
?
-
purified rice LCYe enzyme in a liposome-based biphasic assay system, formation of C4-C5 epsilon-ionone rings. The enzyme from rice produces predominantly monocyclic products and shows very strong OsLCYe activity in vitro. Monocyclic carotene intermediates are not suitable substrates, no activity with 5-cis-lycopene
-
-
?
additional information
?
-
-
purified rice LCYe enzyme in a liposome-based biphasic assay system, formation of C4-C5 epsilon-ionone rings. The enzyme from rice produces predominantly monocyclic products and shows very strong OsLCYe activity in vitro. Monocyclic carotene intermediates are not suitable substrates, no activity with 5-cis-lycopene
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
A447S
like the wild-type enzyme the mutant enzyme adds two epsilon-rings to lycopene, forming epsilon-carotene
L448H
the mutant adds two epsilon-ring to lycopene, forming epsilon-carotene
L448R
the mutant adds two epsilon-ring to lycopene, forming epsilon-carotene
H457D
the mutant enzyme forms a higher amount of ratio delta-carotene compared to the wild-type enzyme
H457L
the mutant adds only one epsilon-ring to lycopene, forming delta-carotene
H457R
like the wild-type enzyme the mutant enzyme predominantly form epsilon-carotene (91%)
H457D
-
the mutant enzyme forms a higher amount of ratio delta-carotene compared to the wild-type enzyme
-
H457L
-
the mutant adds only one epsilon-ring to lycopene, forming delta-carotene
-
H457R
-
like the wild-type enzyme the mutant enzyme predominantly form epsilon-carotene (91%)
-
down
the shift from the beta,epsilon-branch to the beta,beta-branch of the pathway that originates the changes in carotenoid composition during fruit coloration may be explained by a down-regulation of epsilon-LCY and by the increase of the beta-CHX transcript
down
-
the shift from the beta,epsilon-branch to the beta,beta-branch of the pathway that originates the changes in carotenoid composition during fruit coloration may be explained by a down-regulation of epsilon-LCY and by the increase of the beta-CHX transcript
-
additional information
construction and characterization of chimeric epsilon-cyclases of the epsilion-cyclases from Lactuca sativa and Arabidopsis thaliana
additional information
-
construction and characterization of chimeric epsilon-cyclases of the epsilion-cyclases from Lactuca sativa and Arabidopsis thaliana
additional information
construction and characterization of chimeric epsilon-cyclases of the epsilion-cyclases from Lactuca sativa and Arabidopsis thaliana
additional information
-
construction and characterization of chimeric epsilon-cyclases of the epsilion-cyclases from Lactuca sativa and Arabidopsis thaliana
-
additional information
-
for production of lutein in tomato fruits, it is required to overcome the enzyme downregulation before fruit ripening exhibited through a tightly regulated physiological mechanism in tomato fruits by overexpression of lycopene epsilon- and beta-cyclases in leaves and ripe fruits, carotene profile and contents, overview
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
a lycopene beta-cyclase/lycopene epsilon-cyclase/light-harvesting complex-fusion protein is expressed in Escherichia coli BL21(DE3) cells
-
expressed in Arabidopsis thaliana
expression in Escherichia coli
expression in Escherichia coli results in the simultaneous accumulation of alpha- beta-, delta-, and epsilon-carotene
-
gene IbLCYe, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, 4029 bp and 5 introns and 6 exons deduced, real-time quantitative PCR expression analysis, heterologous expression in transgenic Nicotiana tabacum cv. Wisconsin 38 using Agrobacterium tumefaciens strain EHA105 transfection leads to accumulation of significantly increased beta-carotene compared to the untransformed control plants
-
gene Lcy-e, expression in tomato fruits under control of the 35S CaMV promoter in cotyledons via Agrobacterium tumefaciens-mediated transformation, the transgenic plants show increased lutein levels, quantitative real-time quantitative PCR expression analysis
-
gene MpLCYe, DNA and amino acid sequence determination and analysis
recombinant expression in Escherichia coli strain BL21(DE3) of diverse constructs of the enzyme in fusion with GST,HNGW, HXGW, His3 or His6 tags, and in fusion with maltose-binding protein tags, 3C protease cleavage site, N-utilizing substance A, or thioredoxin, for in vivo complementation, the plasmids are transformed into Escherichia coli strain JM109 containing the plasmid pFarbeR enabling lycopene synthesis
single copy gene Czlcy-e, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, heterologous expression and complementation in Escherichia coli strain BL21(DE3)
-
expression in Escherichia coli
expression in Escherichia coli
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Stickforth, P.; Steiger, S.; Hess, W.R.; Sandmann, G.
A novel type of lycopene epsilon-cyclase in the marine cyanobacterium Prochlorococcus marinus MED4
Arch. Microbiol.
179
409-415
2003
Prochlorococcus marinus, Prochlorococcus marinus MED4
brenda
Rodrigo, M.J.; Marcos, J.F.; Zacaras, L.
Biochemical and molecular analysis of carotenoid biosynthesis in flavedo of orange (Citrus sinensis L.) during fruit development and maturation
J. Agric. Food Chem.
52
6724-6731
2004
Citrus sinensis (Q6QM26), Citrus sinensis, Citrus sinensis Osbeck (Q6QM26)
brenda
Cunningham, F.X. Jr.; Pogson, B.; Sun, Z.; McDonald, K.A., DellaPenna, D.; Gantt, E.
Functional analysis of the beta and epsilon lycopene cyclase enzymes of Arabidopsis reveals a mechanism for control of cyclic carotenoid formation
Plant Cell
8
1613-1626
1996
Arabidopsis thaliana (Q38932)
brenda
Cunningham, F.X. Jr.; Gantt, E.
One ring or two? Determination of ring number in carotenoids by lycopene epsilon-cyclases
Proc. Natl. Acad. Sci. USA
98
2905-2910
2001
Arabidopsis thaliana (Q38932), Arabidopsis thaliana, Lactuca sativa (Q9AXK7), Lactuca sativa var. romaine (Q9AXK7)
brenda
Yu, Q.; Beyer, P.
Reaction specificities of the epsilon-ionone-forming lycopene cyclase from rice (Oryza sativa) elucidated in vitro
FEBS Lett.
586
3415-3420
2012
Oryza sativa (Q8LJ81), Oryza sativa
brenda
Yu, L.; Zhai, H.; Chen, W.; He, S.; Liu, Q.
Cloning and functional analysis of lycopene epsilon-cyclase (IbLCYe) gene from Sweetpotato, Ipomoea batatas (L.) Lam
J. Integr. Agric.
12
773-780
2013
Ipomoea batatas
-
brenda
Cordero, B.F.; Couso, I.; Leon, R.; Rodriguez, H.; Vargas, M.A.
Isolation and characterization of a lycopene epsilon-cyclase gene of Chlorella (Chromochloris) zofingiensis. Regulation of the carotenogenic pathway by nitrogen and light
Mar. Drugs
10
2069-2088
2012
Chromochloris zofingiensis (J7Q2X9)
brenda
Giorio, G.; Yildirim, A.; Stigliani, A.L.; DAmbrosio, C.
Elevation of lutein content in tomato: a biochemical tug-of-war between lycopene cyclases
Metab. Eng.
20
167-176
2013
Solanum lycopersicum
brenda
Takemura, M.; Maoka, T.; Misawa, N.
Carotenoid analysis of a liverwort Marchantia polymorpha and functional identification of its lycopene beta- and epsilon-cyclase genes
Plant Cell Physiol.
55
194-200
2014
Marchantia polymorpha (W0SAP4)
brenda
Zunjare, R.; Hossain, F.; Muthusamy, V.; Baveja, A.; Chauhan, H.; Thirunavukkarasu, N.; Saha, S.; Gupta, H.
Influence of rare alleles of beta-carotene hydroxylase and lycopene epsilon cyclase genes on accumulation of provitamin A carotenoids in maize kernels
Plant Breed.
136
872-880
2017
Zea mays
-
brenda
Zunjare, R.U.; Chhabra, R.; Hossain, F.; Baveja, A.; Muthusamy, V.; Gupta, H.S.
Molecular characterization of 5' UTR of the lycopene epsilon cyclase (lcyE) gene among exotic and indigenous inbreds for its utilization in maize biofortification
3 Biotech
8
75
2018
Zea mays
brenda
Shi, Y.; Liu, P.; Xia, Y.; Wei, P.; Li, W.; Zhang, W.; Chen, X.; Cao, P.; Xu, Y.; Jin, L.; Li, F.; Luo, Z.; Wei, C.; Zhang, J.; Xie, X.; Qu, L.; Yang, J.; Lin, F.; Wang, R.
Downregulation of the lycopene epsilon-cyclase gene confers tolerance to salt and drought stress in Nicotiana tabacum
Acta Physiol. Plant.
37
210
2015
Nicotiana tabacum
-
brenda
Zunjare, R.U.; Hossain, F.; Muthusamy, V.; Baveja, A.; Chauhan, H.S.; Bhat, J.S.; Thirunavukkarasu, N.; Saha, S.; Gupta, H.S.
Development of biofortified maize hybrids through marker-assisted stacking of beta-carotene hydroxylase, lycopene-epsilon-cyclase and Opaque2 genes
Front. Plant Sci.
9
178
2018
Zea mays
brenda
Song, X.; Diao, J.; Ji, J.; Wang, G.; Li, Z.; Wu, J.; Josine, T.L.; Wang, Y.
Overexpression of lycopene epsilon-cyclase gene from Lycium chinense confers tolerance to chilling stress in Arabidopsis thaliana
Gene
576
395-403
2016
Lycium chinense (W0NXH4)
brenda
Shi, Y.; Wang, R.; Luo, Z.; Jin, L.; Liu, P.; Chen, Q.; Li, Z.; Li, F.; Wei, C.; Wu, M.; Wei, P.; Xie, H.; Qu, L.; Lin, F.; Yang, J.
Molecular cloning and functional characterization of the lycopene epsilon-cyclase gene via virus-induced gene silencing and its expression pattern in Nicotiana tabacum
Int. J. Mol. Sci.
15
14766-14785
2014
Nicotiana tabacum (F2YGT7)
brenda
Blatt, A.; Bauch, M.E.; Poerschke, Y.; Lohr, M.
A lycopene beta-cyclase/lycopene epsilon-cyclase/light-harvesting complex-fusion protein from the green alga Ostreococcus lucimarinus can be modified to produce alpha-carotene and beta-carotene at different ratios
Plant J.
82
582-595
2015
Ostreococcus sp. 'lucimarinus'
brenda