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Literature summary for 5.4.4.2 extracted from
- The biochemical properties of the two Arabidopsis thaliana isochorismate synthases (2017), Biochem. J., 474, 1579-1590 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ICS1, located on chromosome 1, cloning from seedling cDNA library, recombinant expression of His6-tagged isozyme ICS1 in Escherichia coli strain Rosetta DE3 pLysS | Arabidopsis thaliana |
| gene ICS2, located on chromosome 1, cloning from pFL61 library, functional recombinant expression of His6-tagged isozyme ICS2 in Escherichia coli strain Rosetta DE3 pLysS | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Arabidopsis thaliana | |
| 0.0288 | - |
chorismate | isozyme ICS1, pH 7.0, 33°C | Arabidopsis thaliana |  |
| 0.0343 | - |
chorismate | isozyme ICS1, pH 7.0, 33°C | Arabidopsis thaliana | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | dependent on, Km for ICS1 is 0.942 mM | Arabidopsis thaliana | |
| Mg2+ | dependent on, Km for ICS2 is 0.55 mM | Arabidopsis thaliana | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Chorismate | Arabidopsis thaliana | - |
Isochorismate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q9M9V6 | - |
- |
| Arabidopsis thaliana | Q9S7H8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged isozyme ICS1 from Escherichia coli strain Rosetta DE3 pLysS by nickel affinity chromatography and gel filtration | Arabidopsis thaliana |
| recombinant His6-tagged isozyme ICS2 from Escherichia coli strain Rosetta DE3 pLysS by nickel affinity chromatography and gel filtration | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hydathode | - |
Arabidopsis thaliana | - |
| leaf | - |
Arabidopsis thaliana | - |
| plant vascular cell | isozyme AtICS2 appears to be expressed constitutively, predominantly in the plant vasculature. AtICS2 expression in leaf tissue appears to be localized most strongly in the vasculature and hydathodes | Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Chorismate | - |
Arabidopsis thaliana | Isochorismate | - |
r | |
| additional information | isozyme ICS1 does not act as isochorismate pyruvate lyase (IPL, EC 4.2.99.21) and bifunctional salicylate synthase, it does not convert chorismate into salicylate | Arabidopsis thaliana | ? | - |
? | |
| additional information | isozyme ICS2 does not act as isochorismate pyruvate lyase (IPL, EC 4.2.99.21) and bifunctional salicylate synthase, it does not convert chorismate into salicylate | Arabidopsis thaliana | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, about, recombinant His6-tagged isozyme ICS1, SDS-PAGE | Arabidopsis thaliana |
| ? | x * 60000, about, recombinant His6-tagged isozyme ICS2, SDS-PAGE | Arabidopsis thaliana |
| More | isozyme ICS1 structure comparisons with isozyme ICS2 and other enzymes, overview | Arabidopsis thaliana |
| More | isozyme ICS2 structure comparisons with isozyme ICS1 and other enzymes, overview | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AtICS1 | - |
Arabidopsis thaliana |
| AtICS2 | - |
Arabidopsis thaliana |
| ICS1 | - |
Arabidopsis thaliana |
| ICS2 | - |
Arabidopsis thaliana |
| isochorismate hydroxymutase | - |
Arabidopsis thaliana |
| isochorismate mutase 1 | - |
Arabidopsis thaliana |
| isochorismate mutase 2 | - |
Arabidopsis thaliana |
| isochorismate synthase 1, chloroplastic | UniProt name | Arabidopsis thaliana |
| isochorismate synthase 2, chloroplastic | UniProt name | Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 33 | - |
- |
Arabidopsis thaliana |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
isozymes ICS1 and ICS2 activities display a response to temperature that is more typical of most enzymes, with an approximate doubling in reaction rate per 10°C between 10 and about 33°C, while at temperatures higher than this, reaction rates decline | Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 17 | - |
chorismate | isozyme ICS1, pH 7.0, 33°C | Arabidopsis thaliana | |
| 38.1 | - |
chorismate | isozyme ICS1, pH 7.0, 33°C | Arabidopsis thaliana | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Arabidopsis thaliana |
| 8 | - |
- |
Arabidopsis thaliana |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Arabidopsis thaliana | isozyme ICS2 expression is not induced by salicylate, the AtICS2 promoter activity is not stimulated by virus infection | additional information |
| Arabidopsis thaliana | isozyme ICS1 expression is induced throughout the leaf by exogenous salicylate and virus infection. i.e. cucumber mosaic virus but not with tobacco mosaic virus | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | genes ATICS1 and AtICS2 are both located on chromosome 1 on different sides of the centromere, and they are likely a result of a duplication event, since they are bordered by similar genes. At the DNA sequence level, the protein-coding regions of the two genes share a high degree of similarity, but this does not extend into the untranslated regions | Arabidopsis thaliana |
| metabolism | enzyme ICS does not act as isochorismate pyruvate lyase (IPL, EC 4.2.99.21) and bifunctional salicylate synthase, it does not convert chorismate into salicylate. In Arabidopsis thaliana, salicylate is synthesized from chorismic acid, derived from the shikimic acid pathway, occuring in the plastid | Arabidopsis thaliana |
| additional information | homology modeling of isozymes ICS1 and ICS2 using Serratia marcescens anthranilate synthase (TrpE, PDB ID 1I7Q) as reference structure | Arabidopsis thaliana |
| physiological function | isozyme AtICS1 is required for increased salicylate biosynthesis in response to pathogens, and its expression can be stimulated throughout the leaf by virus infection and exogenous salicylate. Isozymes AtICS1 and AtICS2 can be successful in competing for chorismate in vivo | Arabidopsis thaliana |
| physiological function | isozymes AtICS1 and AtICS2 can be successful in competing for chorismate in vivo | Arabidopsis thaliana |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 590.3 | - |
chorismate | isozyme ICS2, pH 7.0, 33°C | Arabidopsis thaliana | |
| 1110.8 | - |
chorismate | isozyme ICS1, pH 7.0, 33°C | Arabidopsis thaliana | |
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