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Literature summary for 5.1.2.1 extracted from

  • Zhang, X.; Chung, L.W.
    Alternative mechanistic strategy for enzyme catalysis in a Ni-dependent lactate racemase (LarA) intermediate destabilization by the cofactor (2017), Chemistry, 23, 3623-3630 .
    View publication on PubMed
Search for more literature for 5.1.2.1

Metals/Ions

Metals/Ions Comment Organism Structure
Ni2+ required, the Ni-tethered pincer cofactor in enzyme LarA increases reaction barriers and destabilizes NADH-like pyruvate intermediates, due to the less electrophilic Ni cofactor and to the ring strain in the pyruvate intermediates, the Ni ion decreases the electron affinity of cofactor Lactiplantibacillus plantarum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-lactate Lactiplantibacillus plantarum
-
 (R)-lactate
-
 r
(S)-lactate Lactiplantibacillus plantarum ATCC BAA-793 / NCIMB 8826 / WCFS1
-
 (R)-lactate
-
 r

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum F9USS9
-
-
Lactiplantibacillus plantarum ATCC BAA-793 / NCIMB 8826 / WCFS1 F9USS9
-
-

Reaction

Reaction Comment Organism Reaction ID
(S)-Lactate = (R)-lactate the Ni-tethered pincer cofactor in enzyme LarA increases reaction barriers and destabilizes NADH-like pyruvate intermediates, due to the less electrophilic Ni cofactor and to the ring strain in the pyruvate intermediates, reaction mechanism, active and transition state structure, modeling Lactiplantibacillus plantarum
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate
-
 Lactiplantibacillus plantarum (R)-lactate
-
 r
(S)-lactate
-
 Lactiplantibacillus plantarum ATCC BAA-793 / NCIMB 8826 / WCFS1 (R)-lactate
-
 r

Synonyms

Synonyms Comment Organism
LARa
-
 Lactiplantibacillus plantarum

General Information

General Information Comment Organism
additional information the active-site structure is constructed from chain B of the recent crystal structure of Lactobacillus plantarum LarA, PDB ID 5HUQ Lactiplantibacillus plantarum