Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | maximal activity at 0.001 mM | Bordetella pertussis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BLR and KRX cells | Bordetella pertussis |
Protein Variants | Comment | Organism |
---|---|---|
N347A | the mutant shows an enzymatic activity that is reduced by about half as well as 5fold reduced affinity for calmodulin | Bordetella pertussis |
R338A/D360A | the mutant shows no affected catalytic efficiency but 6fold reduced affinity for calmodulin | Bordetella pertussis |
R338A/N347A/D360A | the mutant shows 15% of wild type turnover and exhibits 200fold reduced affinity for calmodulin | Bordetella pertussis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
ATP | wild type enzyme, at pH 8.0 and 30°C | Bordetella pertussis | |
1.5 | - |
ATP | mutant enzyme R338A/D360A, at pH 8.0 and 30°C | Bordetella pertussis | |
2.2 | - |
ATP | mutant enzyme N347A, at pH 8.0 and 30°C | Bordetella pertussis | |
3 | - |
ATP | mutant enzyme R338A/D360A, at pH 8.0 and 30°C | Bordetella pertussis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, SDS-PAGE | Bordetella pertussis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP | Bordetella pertussis | - |
3',5'-cyclic AMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bordetella pertussis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography | Bordetella pertussis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP | - |
Bordetella pertussis | 3',5'-cyclic AMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Bordetella pertussis |
Synonyms | Comment | Organism |
---|---|---|
AC1 | - |
Bordetella pertussis |
adenylyl cyclase | - |
Bordetella pertussis |
Cya | - |
Bordetella pertussis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
650 | - |
ATP | mutant enzyme R338A/D360A, at pH 8.0 and 30°C | Bordetella pertussis | |
2250 | - |
ATP | mutant enzyme N347A, at pH 8.0 and 30°C | Bordetella pertussis | |
4600 | - |
ATP | wild type enzyme, at pH 8.0 and 30°C | Bordetella pertussis | |
6000 | - |
ATP | mutant enzyme R338A/D360A, at pH 8.0 and 30°C | Bordetella pertussis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
200 | - |
ATP | mutant enzyme R338A/D360A, at pH 8.0 and 30°C | Bordetella pertussis | |
1000 | - |
ATP | mutant enzyme N347A, at pH 8.0 and 30°C | Bordetella pertussis | |
4000 | - |
ATP | mutant enzyme R338A/D360A, at pH 8.0 and 30°C | Bordetella pertussis | |
7600 | - |
ATP | wild type enzyme, at pH 8.0 and 30°C | Bordetella pertussis |