Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas putida |
Crystallization (Comment) | Organism |
---|---|
sitting-drop vapor diffusion, 1.25 M ammonium sulfate in 100 mM MES-HCl, pH 6.0 as reservoir solution, protein solution consists of 10-20 mg/ml METase in 120 mM sodium chloride, 10 mM sodium phosphate, pH 7.2, crystals diffract to at least 2.68 A | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine + H2O | Pseudomonas putida | - |
methanethiol + NH3 + 2-oxobutanoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant rMETase | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine + H2O | - |
Pseudomonas putida | methanethiol + NH3 + 2-oxobutanoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
rMETase | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Pseudomonas putida |