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Literature summary for 4.2.3.22 extracted from
- Expression and mechanistic analysis of a germacradienol synthase from Streptomyces coelicolor implicated in geosmin biosynthesis (2003), Proc. Natl. Acad. Sci. USA, 100, 1547-1551.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli strain BL21(DE3) | Streptomyces coelicolor |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | expression of isolated N-terminal and C-terminal domains reveals that the N-terminal domain is responsible for the catalytic activity, while the C-terminal domain is barely active | Streptomyces coelicolor |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Streptomyces coelicolor |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000062 | - |
2-trans,6-trans-farnesyl diphosphate | pH 8.2, 30°C, recombinant enzyme | Streptomyces coelicolor | |
| 0.000115 | - |
2-trans,6-trans-farnesyl diphosphate | pH 8.2, 30°C, recombinant N-terminal domain | Streptomyces coelicolor | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | can substitute for Mg2+ | Streptomyces coelicolor | |
| Cu2+ | can substitute for Mg2+ | Streptomyces coelicolor | |
| Fe2+ | can substitute for Mg2+ | Streptomyces coelicolor | |
| Fe3+ | can substitute for Mg2+ | Streptomyces coelicolor | |
| Mg2+ | required, preferred divalent cation | Streptomyces coelicolor | |
| Mn2+ | can substitute for Mg2+ | Streptomyces coelicolor | |
| additional information | enzyme activity is dependent on divalent cations | Streptomyces coelicolor | |
| Ni2+ | can substitute for Mg2+ | Streptomyces coelicolor | |
| Zn2+ | can substitute for Mg2+ | Streptomyces coelicolor | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40000, recombinant enzyme, SDS-PAGE | Streptomyces coelicolor |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate + H2O | Streptomyces coelicolor | enzyme is involved in geosmin biosynthesis | (1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + diphosphate | - |
? | |
| (2E,6E)-farnesyl diphosphate + H2O | Streptomyces coelicolor A3(2) | enzyme is involved in geosmin biosynthesis | (1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces coelicolor | - |
gene SC9B1.20 | - |
| Streptomyces coelicolor A3(2) | - |
gene SC9B1.20 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain BL21(DE3), soluble enzyme and enzyme solubilized and refolded from inclusion bodies, by ion exchange chromatography and gel filtration | Streptomyces coelicolor |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| (2E,6E)-farnesyl diphosphate + H2O = (1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + diphosphate | reaction mechanism | Streptomyces coelicolor |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| solubilization of recombinant enzyme from inclusion bodies by 0.02% Triton X-100 and 100 mM NaOH, and refolding | Streptomyces coelicolor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate + H2O | enzyme is involved in geosmin biosynthesis | Streptomyces coelicolor | (1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + diphosphate | - |
? | |
| (2E,6E)-farnesyl diphosphate + H2O | cyclization reaction, mechanism of full length enzyme and N-terminal catalytic domain | Streptomyces coelicolor | (1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + diphosphate | - |
? | |
| (2E,6E)-farnesyl diphosphate + H2O | enzyme is involved in geosmin biosynthesis | Streptomyces coelicolor A3(2) | (1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + diphosphate | - |
? | |
| (2E,6E)-farnesyl diphosphate + H2O | cyclization reaction, mechanism of full length enzyme and N-terminal catalytic domain | Streptomyces coelicolor A3(2) | (1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + diphosphate | - |
? | |
| additional information | no activity with geranylgeranyl diphosphate | Streptomyces coelicolor | ? | - |
? | |
| additional information | no activity with geranylgeranyl diphosphate | Streptomyces coelicolor A3(2) | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, recombinant enzyme, SDS-PAGE | Streptomyces coelicolor |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| sesquiterpene synthase | - |
Streptomyces coelicolor |
| terpene cyclase | - |
Streptomyces coelicolor |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Streptomyces coelicolor |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.2 | - |
2-trans,6-trans-farnesyl diphosphate | pH 8.2, 30°C, recombinant N-terminal domain | Streptomyces coelicolor | |
| 6.2 | - |
2-trans,6-trans-farnesyl diphosphate | pH 8.2, 30°C, recombinant enzyme | Streptomyces coelicolor | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
assay at | Streptomyces coelicolor |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.5 | - |
Streptomyces coelicolor |
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