Literature summary for 4.2.2.22 extracted from

Kluskens, L.D.; van Alebeek, G.J.; Voragen, A.G.; de Vos, W.M.; van der Oost, J.
Molecular and biochemical characterization of the thermoactive family 1 pectate lyase from the hyperthermophilic bacterium Thermotoga maritima (2003), Biochem. J., 370, 651-659.

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Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli	Thermotoga maritima

General Stability

General Stability	Organism
Ca2+ stabilizes	Thermotoga maritima

Inhibitors

Inhibitors	Comment	Organism	Structure
EGTA	1 mM, complete inhibition	Thermotoga maritima

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.06	-	polygalacturonic acid	-	Thermotoga maritima

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Thermotoga maritima	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	dependent on	Thermotoga maritima
NaCl	highest activity at 200 mM	Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	4 * 40000, SDS-PAGE	Thermotoga maritima
152000	-	gel filtration	Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
pectin	Thermotoga maritima	the ability of Thermotoga maritima to grow on pectin as sole carbon source coincides with the secretion of a pectate lyase A	?	-	?
pectin	Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589	the ability of Thermotoga maritima to grow on pectin as sole carbon source coincides with the secretion of a pectate lyase A	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	-	-	-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Thermotoga maritima

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
pectin	the ability of Thermotoga maritima to grow on pectin as sole carbon source coincides with the secretion of a pectate lyase A	Thermotoga maritima	?	-	?
pectin	the ability of Thermotoga maritima to grow on pectin as sole carbon source coincides with the secretion of a pectate lyase A	Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589	?	-	?
pectin	pectins with an increasing degree of methylation are degraded at a decreasing rate	Thermotoga maritima	unsaturated trigalacturonate	-	?
pectin	pectins with an increasing degree of methylation are degraded at a decreasing rate	Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589	unsaturated trigalacturonate	-	?
polygalacturonic acid	the enzyme attacks from the reducing end, since only unsaturated trigalacturonic acid is formed, followed by slight formation of unsaturated digalacturonate	Thermotoga maritima	unsaturated trigalacturonate	-	?
polygalacturonic acid	the enzyme attacks from the reducing end, since only unsaturated trigalacturonic acid is formed, followed by slight formation of unsaturated digalacturonate	Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589	unsaturated trigalacturonate	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 40000, SDS-PAGE	Thermotoga maritima

Synonyms

Synonyms	Comment	Organism
exopectate-lyase	-	Thermotoga maritima
pectate lyase A	-	Thermotoga maritima
PelA	-	Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
90	-	-	Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
95	-	half-life: 110 min	Thermotoga maritima
100	-	2 min, 50% loss of activity	Thermotoga maritima
103	-	apparent melting temperature	Thermotoga maritima

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Thermotoga maritima

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Release 2023.1 (January 2023)