Information on EC 4.2.2.22 - pectate trisaccharide-lyase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
4.2.2.22
-
RECOMMENDED NAME
GeneOntology No.
pectate trisaccharide-lyase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
eliminative cleavage of unsaturated trigalacturonate as the major product from the reducing end of polygalacturonic acid/pectate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
SYSTEMATIC NAME
IUBMB Comments
(1->4)-alpha-D-galacturonan reducing-end-trisaccharide-lyase
Differs in specificity from EC 4.2.2.9, pectate disaccharide-lyase, as the predominant action is removal of a trisaccharide rather than a disaccharide from the reducing end. Disaccharides and tetrasaccharides may also be removed [2].
CAS REGISTRY NUMBER
COMMENTARY hide
9015-75-2
cf. EC 4.2.2.2
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GR5
SwissProt
Manually annotated by BRENDA team
strain 14A
SwissProt
Manually annotated by BRENDA team
strain 14A
SwissProt
Manually annotated by BRENDA team
ATCC 35296
SwissProt
Manually annotated by BRENDA team
strain MSB8
-
-
Manually annotated by BRENDA team
subspecies Yersinia enterocolitica 8081
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
PelA knockout mutants do not show significant differences in virulence compared to the wild-type strains during infection of Triticum aestivum plants
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
pectin
?
show the reaction diagram
pectin
unsaturated (1,4-alpha-D-galacturonide)3
show the reaction diagram
pectin
unsaturated trigalacturonate
show the reaction diagram
-
pectins with an increasing degree of methylation are degraded at a decreasing rate
-
-
?
polygalacturonic acid
4,5-unsaturated polygalacturonic acid
show the reaction diagram
polygalacturonic acid
trigalacturonic acid + digalacturonic acid
show the reaction diagram
polygalacturonic acid
unsaturated (1,4-alpha-D-galacturonide)3 + unsaturated (1,4-alpha-D-galacturonide)2
show the reaction diagram
-
-
-
-
?
polygalacturonic acid
unsaturated trigalacturonate
show the reaction diagram
rhamnogalacturonan
?
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
pectin
?
show the reaction diagram
-
the ability of Thermotoga maritima to grow on pectin as sole carbon source coincides with the secretion of a pectate lyase A
-
-
?
polygalacturonic acid
trigalacturonic acid + digalacturonic acid
show the reaction diagram
Q9AQF3
pectate lyase A is a subunit of the extracellular multienzyme complex, called the cellulosome, which is involved in plant cell wall degradation
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CaCl2
optimal activity at 0.05-0.5 mM
NaCl
-
highest activity at 200 mM
additional information
-
utilizes a metal atom other than calcium for catalysis
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CaCl2
1 mM reduces activity to less than that without addition of CaCl2
EGTA
-
1 mM, complete inhibition
polygalacturonic acid
recombinant fusion protein containing a C-terminal His-tag is inhibited by excess substrate
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.06
polygalacturonic acid
-
-
additional information
additional information
Km-value for polygalacturonic acid is 0.56 g/l, recombinant fusion protein containing a C-terminal His-tag
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
Ki-value for polygalacturonic acid is 1.08 g/l
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.37
90% esterified pectin
6.38
60% esterified pectin
57.3
polygalacturonic acid
63.7
30% esterified pectin
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8
pH 7.0: less than 30% of maximal activity, pH 8.0: optimum
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
65
recombinant fusion protein containing a C-terminal His-tag
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40 - 60
more than 50% of maximal activity at 40°C and at 60°C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33451
x * 33451, calculated from sequence
36000
x * 36000, SDS-PAGE
42000
x * 42000, SDS-PAGE
152000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
-
4 * 40000, SDS-PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drop vapour diffusion method, in 30% polyethylene glycol 1500 and 0.1 M sodium citrate, pH 5.0
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 10
at 50°C
682998
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
95
-
half-life: 110 min
100
-
2 min, 50% loss of activity
103
-
apparent melting temperature
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Ca2+ stabilizes
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
the recombinant protein using Ni2+-nitrilotriacetateagarose column chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expression in Escherichia coli
-
expression in Escherichia coli as a recombinant fusion protein containing a C-terminal His-tag
overexpression in Escherichia coli
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
construction of PelA knockout mutants
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
industry