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Literature summary for 4.2.1.84 extracted from

  • Sun, W.; Zhu, L.; Chen, X.; Wu, L.; Zhou, Z.; Liu, Y.
    The stability enhancement of nitrile hydratase from Bordetella petrii by swapping the C-terminal domain of beta subunit (2016), Appl. Biochem. Biotechnol., 178, 1481-1487 .
    View publication on PubMed
Search for more literature for 4.2.1.84

Cloned(Commentary)

Cloned (Comment) Organism
-
 Bordetella petrii
-
 Pseudonocardia thermophila

Protein Variants

Protein Variants Comment Organism
additional information the chimeric NHase (SBpNHase) from the thermal sensitive nitrile hydratasese from Bordetella petrii and the relatively thermal-stable nitrile hydratases from Pseudonocardia thermophila is constructed by swapping the corresponding C-domains Bordetella petrii
additional information the chimeric NHase (SBpNHase) from the thermal sensitive nitrile hydratasese from Bordetella petrii and the relatively thermal-stable nitrile hydratases from Pseudonocardia thermophila is constructed by swapping the corresponding C-domains Pseudonocardia thermophila

Organism

Organism UniProt Comment Textmining
Bordetella petrii A9IEH0 AND A9IEH2 A9IEH0: beta subunit, A9IEH2: alpha subunit
-
Bordetella petrii DSM 128043 A9IEH0 AND A9IEH2 A9IEH0: beta subunit, A9IEH2: alpha subunit
-
Pseudonocardia thermophila Q7SID3 AND Q7SID2 Q7SID3: beta subunit, Q7SID2: alpha subunit
-

Purification (Commentary)

Purification (Comment) Organism
the chimeric NHase (SBpNHase) from the thermal sensitive nitrile hydratasese from Bordetella petrii and the relatively thermal-stable nitrile hydratases from Pseudonocardia thermophila is constructed by swapping the corresponding C-domains Bordetella petrii
the chimeric NHase (SBpNHase) from the thermal sensitive nitrile hydratasese from Bordetella petrii and the relatively thermal-stable nitrile hydratases from Pseudonocardia thermophila is constructed by swapping the corresponding C-domains Pseudonocardia thermophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 30 min, nearly complete loss of activity of the native enzyme. Chimeric nitrile hydratases (SBpNHase) from the thermal sensitive nitrile hydratasese from Bordetella petrii and the relatively thermal-stable nitrile hydratases from Pseudonocardia thermophila (constructed by swapping the corresponding C-domains) retains 50% residual activity Bordetella petrii
50
-
 chimeric nitrile hydratases (SBpNHase) from the thermal sensitive nitrile hydratasese from Bordetella petrii and the relatively thermal-stable nitrile hydratases from Pseudonocardia thermophila (constructed by swapping the corresponding C-domains) retains 50% residual activity Pseudonocardia thermophila
55
-
 melting temperature of the native enzyme Pseudonocardia thermophila