Literature summary for 4.2.1.56 extracted from

Sasikaran, J.; Ziemski, M.; Zadora, P.K.; Fleig, A.; Berg, I.A.
Bacterial itaconate degradation promotes pathogenicity (2014), Nat. Chem. Biol., 10, 371-377.

Search for more literature for 4.2.1.56

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Pseudomonas aeruginosa
expressed in Escherichia coli BL21(DE3) cells	Yersinia pestis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.034	-	itaconyl-CoA	at pH 7.5 and 45°C	Pseudomonas aeruginosa
0.12	-	itaconyl-CoA	at pH 7.5 and 45°C	Yersinia pestis
0.14	-	(S)-citramalyl-CoA	at pH 7.5 and 45°C	Pseudomonas aeruginosa
0.31	-	mesaconyl-C4-CoA	at pH 7.5 and 45°C	Pseudomonas aeruginosa
0.49	-	(S)-citramalyl-CoA	at pH 7.5 and 45°C	Yersinia pestis
0.51	-	mesaconyl-C4-CoA	at pH 7.5 and 45°C	Yersinia pestis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	the enzyme does not require divalent metal ions	Pseudomonas aeruginosa
additional information	the enzyme does not require divalent metal ions	Yersinia pestis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
itaconyl-CoA + H2O	Pseudomonas aeruginosa	-	(S)-citramalyl-CoA	-	r
itaconyl-CoA + H2O	Yersinia pestis	-	(S)-citramalyl-CoA	-	r
itaconyl-CoA + H2O	Yersinia pestis Y2384	-	(S)-citramalyl-CoA	-	r
itaconyl-CoA + H2O	Pseudomonas aeruginosa PA0878	-	(S)-citramalyl-CoA	-	r

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	-	-	-
Pseudomonas aeruginosa PA0878	-	-	-
Yersinia pestis	-	-	-
Yersinia pestis Y2384	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-citramalyl-CoA	-	Pseudomonas aeruginosa	itaconyl-CoA + H2O	-	r
(S)-citramalyl-CoA	-	Yersinia pestis	itaconyl-CoA + H2O	-	r
(S)-citramalyl-CoA	-	Yersinia pestis Y2384	itaconyl-CoA + H2O	-	r
(S)-citramalyl-CoA	-	Pseudomonas aeruginosa PA0878	itaconyl-CoA + H2O	-	r
itaconyl-CoA + H2O	-	Pseudomonas aeruginosa	(S)-citramalyl-CoA	-	r
itaconyl-CoA + H2O	-	Yersinia pestis	(S)-citramalyl-CoA	-	r
itaconyl-CoA + H2O	-	Yersinia pestis Y2384	(S)-citramalyl-CoA	-	r
itaconyl-CoA + H2O	-	Pseudomonas aeruginosa PA0878	(S)-citramalyl-CoA	-	r
mesaconyl-C4-CoA + H2O	-	Pseudomonas aeruginosa	itaconyl-CoA	-	r
mesaconyl-C4-CoA + H2O	-	Yersinia pestis	itaconyl-CoA	-	r
mesaconyl-C4-CoA + H2O	-	Yersinia pestis Y2384	itaconyl-CoA	-	r
mesaconyl-C4-CoA + H2O	-	Pseudomonas aeruginosa PA0878	itaconyl-CoA	-	r

Synonyms

Synonyms	Comment	Organism
ICH	-	Pseudomonas aeruginosa
ICH	-	Yersinia pestis
RipB	-	Pseudomonas aeruginosa
RipB	-	Yersinia pestis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
4.4	-	(S)-citramalyl-CoA	at pH 7.5 and 45°C	Yersinia pestis
52.2	-	itaconyl-CoA	at pH 7.5 and 45°C	Yersinia pestis
98.8	-	mesaconyl-C4-CoA	at pH 7.5 and 45°C	Yersinia pestis
415	-	itaconyl-CoA	at pH 7.5 and 45°C	Pseudomonas aeruginosa
415.4	-	(S)-citramalyl-CoA	at pH 7.5 and 45°C	Pseudomonas aeruginosa
4651	-	mesaconyl-C4-CoA	at pH 7.5 and 45°C	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)