Information on EC 4.2.1.56 - Itaconyl-CoA hydratase

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The expected taxonomic range for this enzyme is: Proteobacteria

EC NUMBER
COMMENTARY
4.2.1.56
-
RECOMMENDED NAME
GeneOntology No.
Itaconyl-CoA hydratase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Citramalyl-CoA = itaconyl-CoA + H2O
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
elimination
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-
-
-
PATHWAY
KEGG Link
MetaCyc Link
C5-Branched dibasic acid metabolism
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itaconate degradation
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SYSTEMATIC NAME
IUBMB Comments
citramalyl-CoA hydro-lyase (itaconyl-CoA-forming)
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SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Hydratase, itaconyl coenzyme A
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-
-
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ICH
Pseudomonas aeruginosa PA0878
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-
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ICH
Yersinia pestis Y2384
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-
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Itaconyl coenzyme A hydratase
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-
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Itaconyl-CoA hydratase
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-
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RipB
Pseudomonas aeruginosa PA0878
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-
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RipB
Yersinia pestis Y2384
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-
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CAS REGISTRY NUMBER
COMMENTARY
37290-83-8
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Pseudomonas aeruginosa PA0878
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-
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Manually annotated by BRENDA team
Yersinia pestis Y2384
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-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-citramalyl-CoA
itaconyl-CoA + H2O
show the reaction diagram
Pseudomonas aeruginosa, Yersinia pestis, Yersinia pestis Y2384, Pseudomonas aeruginosa PA0878
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-
-
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r
Itaconyl-CoA + H2O
Citramalyl-CoA
show the reaction diagram
Pseudomonas sp., Pseudomonas sp. B2aba
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-
-
-
Itaconyl-CoA + H2O
?
show the reaction diagram
Pseudomonas sp., Pseudomonas sp. B2aba
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enzyme is involved in itaconate catabolism
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-
-
itaconyl-CoA + H2O
(S)-citramalyl-CoA
show the reaction diagram
Pseudomonas aeruginosa, Yersinia pestis, Yersinia pestis Y2384, Pseudomonas aeruginosa PA0878
-
-
-
-
r
mesaconyl-C4-CoA + H2O
itaconyl-CoA
show the reaction diagram
Pseudomonas aeruginosa, Yersinia pestis, Yersinia pestis Y2384, Pseudomonas aeruginosa PA0878
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r
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Itaconyl-CoA + H2O
?
show the reaction diagram
-
enzyme is involved in itaconate catabolism
-
-
-
itaconyl-CoA + H2O
(S)-citramalyl-CoA
show the reaction diagram
Pseudomonas aeruginosa, Yersinia pestis, Yersinia pestis Y2384, Pseudomonas aeruginosa PA0878
-
-
-
-
r
Itaconyl-CoA + H2O
?
show the reaction diagram
Pseudomonas sp. B2aba
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enzyme is involved in itaconate catabolism
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-
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METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
the enzyme does not require divalent metal ions
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.14
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(S)-citramalyl-CoA
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at pH 7.5 and 45C
0.49
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(S)-citramalyl-CoA
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at pH 7.5 and 45C
0.034
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itaconyl-CoA
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at pH 7.5 and 45C
0.12
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itaconyl-CoA
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at pH 7.5 and 45C
0.31
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mesaconyl-C4-CoA
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at pH 7.5 and 45C
-
0.51
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mesaconyl-C4-CoA
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at pH 7.5 and 45C
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kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
4.4
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(S)-citramalyl-CoA
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at pH 7.5 and 45C
90061
415.4
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(S)-citramalyl-CoA
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at pH 7.5 and 45C
90061
52.2
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itaconyl-CoA
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at pH 7.5 and 45C
10683
415
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itaconyl-CoA
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at pH 7.5 and 45C
10683
98.8
-
mesaconyl-C4-CoA
-
at pH 7.5 and 45C
0
4651
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mesaconyl-C4-CoA
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at pH 7.5 and 45C
0
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
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