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Literature summary for 4.2.1.153 extracted from

  • Zarzycki, J.; Schlichting, A.; Strychalsky, N.; Muller, M.; Alber, B.E.; Fuchs, G.
    Mesaconyl-coenzyme A hydratase, a new enzyme of two central carbon metabolic pathways in bacteria (2008), J. Bacteriol., 190, 1366-1374.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.153

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Cereibacter sphaeroides
expressed in Escherichia coli BL21 cells Chloroflexus aurantiacus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 x * 37000, SDS-PAGE Cereibacter sphaeroides
38000
-
 x * 38000, SDS-PAGE Chloroflexus aurantiacus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-methylmalyl-CoA Cereibacter sphaeroides
-
 mesaconyl-CoA + H2O
-
 ?
beta-methylmalyl-CoA Chloroflexus aurantiacus
-
 mesaconyl-CoA + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-
Chloroflexus aurantiacus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose column chromatography Chloroflexus aurantiacus
Ni-Sepharose column chromatography and Superdex 200 gel filtration Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-methylmalyl-CoA
-
 Cereibacter sphaeroides mesaconyl-CoA + H2O
-
 ?
beta-methylmalyl-CoA
-
 Chloroflexus aurantiacus mesaconyl-CoA + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE Chloroflexus aurantiacus
? x * 37000, SDS-PAGE Cereibacter sphaeroides

Synonyms

Synonyms Comment Organism
mesaconyl-CoA hydratase
-
 Cereibacter sphaeroides
mesaconyl-CoA hydratase
-
 Chloroflexus aurantiacus
mesaconyl-coenzyme A hydratase
-
 Cereibacter sphaeroides
mesaconyl-coenzyme A hydratase
-
 Chloroflexus aurantiacus