Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.146 extracted from

  • Kuorelahti, S.; Jouhten, P.; Maaheimo, H.; Penttilae, M.; Richard, P.
    L-galactonate dehydratase is part of the fungal path for D-galacturonic acid catabolism (2006), Mol. Microbiol., 61, 1060-1068.
    View publication on PubMed
Search for more literature for 4.2.1.146

Cloned(Commentary)

Cloned (Comment) Organism
the LGD1 protein is tagged with a histidine tag and produced in Saccharomyces cerevisiae. In one construct the C-terminus of the protein was tagged, in another the N-terminal end of the protein Trichoderma reesei

Protein Variants

Protein Variants Comment Organism
additional information the C-terminal tagging results in an inactive protein while the N-terminal tagging results in a protein with reduced activity. The activity found in the crude extract of the N-terminally tagged protein is about five times lower than the non-tagged protein Trichoderma reesei

Inhibitors

Inhibitors Comment Organism Structure
EDTA 5 mM, complete loss of activity Trichoderma reesei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5
-
 L-galactonate pH 7.0 Trichoderma reesei

Metals/Ions

Metals/Ions Comment Organism Structure
additional information divalent cation required Trichoderma reesei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-galactonate Trichoderma reesei the second enzyme of the fungal pathway for D-galacturonic acid catabolism after the D-galacturonic acid reductase. A deletion of the L-galactonate dehydratase gene in Hypocrea jecorina results in a strain with no growth on D-galacturonic acid 2-dehydro-3-deoxy-L-galactonate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Trichoderma reesei Q0QWS4 Trichoderma reesei
-

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:L-galactonate-grown cell L-galactonate dehydratase activity is present in cells grown on D-galacturonic acid but absent when other carbon sources are used for growth Trichoderma reesei
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-arabonate 50% of the activity with L-galactonate Trichoderma reesei ?
-
 ?
L-galactonate
-
 Trichoderma reesei 2-dehydro-3-deoxy-L-galactonate + H2O
-
 ?
L-galactonate the second enzyme of the fungal pathway for D-galacturonic acid catabolism after the D-galacturonic acid reductase. A deletion of the L-galactonate dehydratase gene in Hypocrea jecorina results in a strain with no growth on D-galacturonic acid Trichoderma reesei 2-dehydro-3-deoxy-L-galactonate + H2O
-
 ?
additional information no activity with D-galactonate, D-gluconate, L-gulonate or D-xylonate Trichoderma reesei ?
-
 ?

Synonyms

Synonyms Comment Organism
LGD1
-
 Trichoderma reesei