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Literature summary for 4.2.1.139 extracted from
- The pterocarpan synthase of alfalfa: association and co-induction of vestitone reductase and 7,2-dihydroxy-4-methoxy-isoflavanol (DMI) dehydratase, the two final enzymes in medicarpin biosynthesis (1994), FEBS Lett., 356, 221-225.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
gel filtration, mixture of vestitone reductase and 7,2-dihydroxy-4-methoxyisoflavanol dehydratase, presence of 100 mM KCl | Medicago sativa |
| 70000 | - |
gel filtration, mixture of vestitone reductase and 7,2-dihydroxy-4-methoxyisoflavanol dehydratase, absence of KCl | Medicago sativa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Medicago sativa | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| callus | - |
Medicago sativa | - |
| cell suspension culture | - |
Medicago sativa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,2-dihydroxy-4-methoxyisoflavanol | - |
Medicago sativa | (-)-medicarpin + H2O | i.e. 6aR,11aR-medicarpin | ? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 7,2-dihydroxy-4-methoxyisoflavanol dehydratase | - |
Medicago sativa |
| DMI dehydratase | - |
Medicago sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Medicago sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Medicago sativa |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Medicago sativa | the activities of vestitone reductase and 7,2'-dihydroxy-4'-methoxyisoflavanol dehydratase increase approximately 3fold 6 hours after elicitor treatment in alfalfa suspension cell culture. The activities remain at maximal levels for 40 hours, correlating with a steady increase in the medicarpin content of the cells | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | 7,2-dihydroxy-4-methoxyisoflavanol dehydratase is the final enzyme in medicarpin biosynthesis in alfalfa | Medicago sativa |
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