Information on EC 4.2.1.139 - medicarpin synthase

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The expected taxonomic range for this enzyme is: Medicago sativa

EC NUMBER
COMMENTARY hide
4.2.1.139
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RECOMMENDED NAME
GeneOntology No.
medicarpin synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4'-methoxyisoflavan-2',4,7-triol = (-)-medicarpin + H2O
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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glyceollin biosynthesis I
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Isoflavonoid biosynthesis
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maackiain biosynthesis
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medicarpin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
2',7-dihydroxy-4'-methoxyisoflavanol hydro-lyase [(–)-medicarpin-forming]
Isolated from the plant Medicago sativa (alfalfa). Catalyses the final step in the biosynthesis of medicarpin, the main pterocarpan phytoalexin in alfalfa.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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7,2-dihydroxy-4-methoxyisoflavanol dehydratase is the final enzyme in medicarpin biosynthesis in alfalfa
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
7,2-dihydroxy-4-methoxyisoflavanol
(-)-medicarpin + H2O
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05
7,2-dihydroxy-4-methoxyisoflavanol
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pH 6.0, 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2020
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pH 6.0, 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70000
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gel filtration, mixture of vestitone reductase and 7,2-dihydroxy-4-methoxyisoflavanol dehydratase, absence of KCl
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the activities of vestitone reductase and 7,2'-dihydroxy-4'-methoxyisoflavanol dehydratase increase approximately 3fold 6 hours after elicitor treatment in alfalfa suspension cell culture. The activities remain at maximal levels for 40 hours, correlating with a steady increase in the medicarpin content of the cells
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