Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Mus musculus |
expressed in Escherichia coli as a His-tagged fusion protein | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Mus musculus | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate and further purification using three successive chromatographic steps on Blue Trisacryl, Source 30Q and superdex 200 | Mus musculus |
ammonium sulfate and further purification using three successive chromatographic steps on Blue Trisacryl, Source 30Q and superdex 200 | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + NADHX | YjeF is bi-functional and ADP-dependent. YjeF not only dehydrates (S)-NAD(P)HX, but also interconverts (R)- and (S)-NAD(P)HX. For YjeF, (S)-NADPHX is a better substrate for this enzyme than (S)-NADHX, as indicated by a 2fold higher catalytic efficiency with (S)-NADPHX | Escherichia coli | AMP + phosphate + NADH | - |
? | |
ATP + NADHX | the resulting mixture contains, in addition to (S)-NAD(P)HX and (R)-NAD(P)HX (in a 60:40 ratio), non-reacted NAD(P)H and cyclic forms derived from NAD(P)HX. (S)-NADPHX is a better substrate for this enzyme than (S)-NADHX, as indicated by a 2fold higher catalytic efficiency with (S)-NADPHX | Saccharomyces cerevisiae | ADP + phosphate + NADH | - |
? | |
ATP + NADHX | the resulting mixture contains, in addition to (S)-NAD(P)HX and (R)-NAD(P)HX (in a 60:40 ratio), non-reacted NAD(P)H and cyclic forms derived from NAD(P)HX. For Carkd, a 4fold higher Vmax value is found when (S)-NADPHX is used as a substrate instead of (S)-NADHX | Mus musculus | ADP + phosphate + NADH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Carkd | - |
Mus musculus |
NADHX dehydratase | - |
Mus musculus |
NADHX dehydratase | - |
Saccharomyces cerevisiae |
YjeF | - |
Escherichia coli |
YKL151C | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mus musculus |
30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
assay at | Mus musculus |
7.1 | - |
assay at | Saccharomyces cerevisiae |