Information on EC 4.2.1.136 - ADP-dependent NAD(P)H-hydrate dehydratase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
4.2.1.136
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RECOMMENDED NAME
GeneOntology No.
ADP-dependent NAD(P)H-hydrate dehydratase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ADP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = AMP + phosphate + NADH
show the reaction diagram
(1)
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ADP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate = AMP + phosphate + NADPH
show the reaction diagram
(2)
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
AMP + phosphate + NADH
show the reaction diagram
ADP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
AMP + phosphate + NADPH
show the reaction diagram
ADP + NADHX
AMP + phosphate + NADH
show the reaction diagram
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YjeF is bi-functional and ADP-dependent. YjeF not only dehydrates (S)-NAD(P)HX, but also interconverts (R)- and (S)-NAD(P)HX. For YjeF, (S)-NADPHX is a better substrate for this enzyme than (S)-NADHX, as indicated by a 2fold higher catalytic efficiency with (S)-NADPHX
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?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
ADP + phosphate + NADH
show the reaction diagram
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
ADP + phosphate + NADPH
show the reaction diagram
ATP + NADHX
ADP + phosphate + NADH
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
ADP + phosphate + NADH
show the reaction diagram
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
ADP + phosphate + NADPH
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00086 - 0.00115
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
0.00069 - 0.00089
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.26 - 0.3
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
0.34 - 0.38
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
262 - 358
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
27689
509 - 524
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
37366
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
PDB
SCOP
CATH
ORGANISM
UNIPROT
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Enterococcus faecalis (strain ATCC 700802 / V583)
Enterococcus faecalis (strain ATCC 700802 / V583)
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified recombinant protein YxkO, hanging drop method, 1:1 mixture of a solution containing 25 mg/ml protein and 10 mM ATP with the crystallization buffer composed of 13.5% v/v PEG 400, 0.09 M MgCl2, 5% glycerol and 0.045 M HEPES, pH 7.5, crystal soaking with metabolite cocktails, X-ray diffraction structure determination and analysis at 1.5-1.9 A resolution, analysis of the PF01256 site
purified recombinant protein Tm0922, hanging drop method, 1:1 mixture of a solution containing 25 mg/ml protein and 10 mM ATP with the crystallization buffer composed of 13.5% v/v PEG 400, 0.09 M MgCl2, 5% glycerol, and 0.045 M HEPES, pH 7.5, crystal soaking with metabolite cocktails, X-ray diffraction structure determination and analysis at 2.05-2.8 A resolution, analysis of the PF01256 site
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate and further purification using three successive chromatographic steps on Blue Trisacryl, Source 30Q and superdex 200
recombinant N-terminally His-tagged mature dehydratase from Escherichia coli strain BL21 (DE3) RIPL by nickel affinity chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli as a His-tagged fusion protein
transient expression of GFP-tagged enzyme in Nicotiana tabacum BY-2 cells, expression of N-terminally His-tagged mature dehydratase in Escherichia coli strain BL21 (DE3) RIPL
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Show AA Sequence (11867 entries)
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