Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme activity requires a reductant for activation, influence of different reducing agents on Lis activity, overview | Thauera linaloolentis |
Cloned (Comment) | Organism |
---|---|
gene lis, recombinant expression in Escherichia coli strain BL21(DE3) in membranes | Thauera linaloolentis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression of a N-terminally truncated version of the linalool isomerase, representing the cytosolic part of the enzyme only, yields a soluble protein that does not show activity | Thauera linaloolentis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | detergent-mediated release of the protein from the membrane causes irreversible inhibitory effects on the enzyme activity | Thauera linaloolentis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten-kinetics | Thauera linaloolentis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
inner membrane | membrane-anchored enzyme | Thauera linaloolentis | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
600000 | - |
gel filtration | Thauera linaloolentis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R,S)-linalool | Thauera linaloolentis | - |
beta-myrcene + H2O | - |
r | |
(R,S)-linalool | Thauera linaloolentis 47Lol | - |
beta-myrcene + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thauera linaloolentis | - |
- |
- |
Thauera linaloolentis 47Lol | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from the inner membrane using subcellular fractionation including a sucrose-gradient centrifugation and ultracentrifugation at 150000 x g, anion exchange and hydrophobic interaction chromatography, and gel filtration, the detergent-mediated release of the protein from the membrane has irreversible inhibitory effects on the enzyme activity, spheroplast preparation | Thauera linaloolentis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R,S)-linalool | - |
Thauera linaloolentis | beta-myrcene + H2O | - |
r | |
(R,S)-linalool | the enzyme is not stereospecific | Thauera linaloolentis | beta-myrcene + H2O | - |
r | |
(R,S)-linalool | - |
Thauera linaloolentis 47Lol | beta-myrcene + H2O | - |
r | |
(R,S)-linalool | the enzyme is not stereospecific | Thauera linaloolentis 47Lol | beta-myrcene + H2O | - |
r | |
additional information | a bifunctional linalool dehydratase/isomerase that exhibits dehydratase activity, Ec 4.2.1.127, and isomerase activity, EC 5.4.4.4. The enzyme catalyzes the reversible hydration of beta-myrcene to linalool and its isomerization to geraniol. No activity is measured with nerol or citronellol alone. Linalool isomerase activity drops to approx. 50% in the presence of nerol. Activity in the presence of citronellol and geraniol is barely detectable. The enzyme is regioselective and seems to bind nerol with a similar affinity as geraniol, whereas citronellol which lacks the C2-C3 double bond is stronger bound than geraniol. Stereoselectivity analysis | Thauera linaloolentis | ? | - |
? | |
additional information | a bifunctional linalool dehydratase/isomerase that exhibits dehydratase activity, Ec 4.2.1.127, and isomerase activity, EC 5.4.4.4. The enzyme catalyzes the reversible hydration of beta-myrcene to linalool and its isomerization to geraniol. No activity is measured with nerol or citronellol alone. Linalool isomerase activity drops to approx. 50% in the presence of nerol. Activity in the presence of citronellol and geraniol is barely detectable. The enzyme is regioselective and seems to bind nerol with a similar affinity as geraniol, whereas citronellol which lacks the C2-C3 double bond is stronger bound than geraniol. Stereoselectivity analysis | Thauera linaloolentis 47Lol | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | 10 x 60000, recombinant enzyme, SDS-PAGE, 10 x 71800, about, sequence calculation | Thauera linaloolentis |
Synonyms | Comment | Organism |
---|---|---|
linalool dehydratase/isomerase | - |
Thauera linaloolentis |
LIS | - |
Thauera linaloolentis |
More | cf. EC 5.4.4.4 | Thauera linaloolentis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Thauera linaloolentis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Thauera linaloolentis |