Information on EC 4.2.1.127 - linalool dehydratase

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The expected taxonomic range for this enzyme is: Castellaniella defragrans

EC NUMBER
COMMENTARY hide
4.2.1.127
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RECOMMENDED NAME
GeneOntology No.
linalool dehydratase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(3S)-linalool = myrcene + H2O
show the reaction diagram
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
beta; myrcene degradation
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Biosynthesis of secondary metabolites
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Monoterpenoid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
linalool hydro-lyase (myrcene-forming)
In absence of oxygen the bifunctional linalool dehydratase-isomerase can catalyse in vitro two reactions, the hydration of myrcene to (3S)-linalool and the isomerization of (3S)-linalool to geraniol, the latter activity being classified as EC 5.4.4.4, geraniol isomerase.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
beta-myrcene + H2O
(3S)-linalool
show the reaction diagram
linalool
myrcene + H2O
show the reaction diagram
myrcene + H2O
linalool
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
beta-myrcene + H2O
(3S)-linalool
show the reaction diagram
myrcene + H2O
linalool
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
contains no prosthetic group
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KCl
high salt concentrations (NaCl, KCl, or MgCl2 at a concentration of 220 mM) inhibit the enzyme activity completely
MgCl2
high salt concentrations (NaCl, KCl, or MgCl2 at a concentration of 220 mM) inhibit the enzyme activity completely
NaCl
high salt concentrations (NaCl, KCl, or MgCl2 at a concentration of 220 mM) inhibit the enzyme activity completely
O2
the enzyme requires dithiothreitol as a reducing agent and an oxygen free microenvironment (less than 1% (v/v)) for the dehydration of linalool
pyridoxal 5'-phosphate
1 mM; 35% inhibition
S-adenosyl-L-methionine
40 mM, 80% inhibition
Ti(III)citrate
1 mM, complete inhibition
Urea
20% activity remains at 3 M urea, and no activity is detected in 6 M urea
additional information
not inhibited by 10% (v/v) DMSO. EDTA (5 mM) does not affect the enzyme activity
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dithiothreitol
the aerobically purified enzyme is anaerobically activated in the presence of 2 mM dithiothreitol
additional information
the enzyme requires dithiothreitol as a reducing agent and an oxygen free microenvironment (less than 1% (v/v)) for the dehydration of linalool
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.75
linalool
pH 9.0, 35°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28 - 40
28°C: about 60% of maximal activity, 40°C: about 80% of maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
4 * 40000, SDS-PAGE
160000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
proteome analysis of cells grown on acetate and alpha-phellandrene by 2D-SDS-PAGE and LC-ESI-MS/MS of whole-cell homogenate and membrane protein-enriched fraction, overview
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information