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Literature summary for 4.2.1.108 extracted from

  • Rajan, L.A.; Joseph, T.C.; Thampuran, N.; James, R.; Kumar, K.A.; Viswanathan, C.; Bansal, K.C.
    Cloning and heterologous expression of ectoine biosynthesis genes from Bacillus halodurans in Escherichia coli (2008), Biotechnol. Lett., 30, 1403-1407.
    View publication on PubMed
Search for more literature for 4.2.1.108

Application

Application Comment Organism
biotechnology ectoine has importance in dermopharmacy as anti ageing agents in skin creams, as components of shampoo, for oral care and as adjuvants for vaccines Halalkalibacterium halodurans

Cloned(Commentary)

Cloned (Comment) Organism
the ectABC gene cluster is expressed in Escherichia coli M15 (pREP4) by vector pQE30 Halalkalibacterium halodurans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15000
-
 SDS-PAGE, calculated Halalkalibacterium halodurans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N4-acetyl-L-2,4-diaminobutyrate Halalkalibacterium halodurans
-
 2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
-
 ?
N4-acetyl-L-2,4-diaminobutyrate Halalkalibacterium halodurans
-
 L-ectoine + H2O
-
 ?
N4-acetyl-L-2,4-diaminobutyrate Halalkalibacterium halodurans TCJAR021
-
 2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
-
 ?
N4-acetyl-L-2,4-diaminobutyrate Halalkalibacterium halodurans TCJAR021
-
 L-ectoine + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Halalkalibacterium halodurans A5A5G3 the ectABC gene cluster expressed in Escherichia coli M15 (pREP4)
-
Halalkalibacterium halodurans TCJAR021 A5A5G3 the ectABC gene cluster expressed in Escherichia coli M15 (pREP4)
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N4-acetyl-L-2,4-diaminobutyrate
-
 Halalkalibacterium halodurans 2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
-
 ?
N4-acetyl-L-2,4-diaminobutyrate
-
 Halalkalibacterium halodurans TCJAR021 2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
-
 ?
N4-acetyl-L-2,4-diaminobutyrate
-
 Halalkalibacterium halodurans L-ectoine + H2O
-
 ?
N4-acetyl-L-2,4-diaminobutyrate
-
 Halalkalibacterium halodurans TCJAR021 L-ectoine + H2O
-
 ?

Synonyms

Synonyms Comment Organism
ectoine synthase
-
 Halalkalibacterium halodurans

pI Value

Organism Comment pI Value Maximum pI Value
Halalkalibacterium halodurans calculated
-
 4.93