Information on EC 4.2.1.108 - ectoine synthase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.2.1.108
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RECOMMENDED NAME
GeneOntology No.
ectoine synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N4-acetyl-L-2,4-diaminobutanoate = L-ectoine + H2O
show the reaction diagram
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
condensation
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ectoine biosynthesis
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Glycine, serine and threonine metabolism
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Metabolic pathways
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
N4-acetyl-L-2,4-diaminobutanoate hydro-lyase (L-ectoine-forming)
Ectoine is an osmoprotectant that is found in halophilic eubacteria. This is the third enzyme in the ectoine-biosynthesis pathway, the other enzymes involved being EC 2.6.1.76, diaminobutyrate---2-oxoglutarate transaminase and EC 2.3.1.178, diaminobutyrate acetyltransferase [1,2].
CAS REGISTRY NUMBER
COMMENTARY hide
130457-09-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain DSM 485
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-
Manually annotated by BRENDA team
formerly Halomonas elongata, strain DSM 3043, gene ectC encoded in the ectABC gene cluster
SwissProt
Manually annotated by BRENDA team
gene ectC
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-
Manually annotated by BRENDA team
extremely halophilic eubacterium, strain DSM 1059
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-
Manually annotated by BRENDA team
gene ectC
SwissProt
Manually annotated by BRENDA team
gene ectC
UniProt
Manually annotated by BRENDA team
gene ectC
UniProt
Manually annotated by BRENDA team
gene ectC
UniProt
Manually annotated by BRENDA team
gene ectC
UniProt
Manually annotated by BRENDA team
i.e. Methylobacter alcaliphilus, gene ectC
UniProt
Manually annotated by BRENDA team
gene ectC
UniProt
Manually annotated by BRENDA team
gene ectC
UniProt
Manually annotated by BRENDA team
gene ectC
UniProt
Manually annotated by BRENDA team
gene ectC
UniProt
Manually annotated by BRENDA team
gene ectC
UniProt
Manually annotated by BRENDA team
no activity in Aneurinibacillus aneurinilyticus
strain DSM 5562, formerly Bacillus aneurinilyticus
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-
Manually annotated by BRENDA team
no activity in Aneurinibacillus aneurinilyticus DSM 5562
strain DSM 5562, formerly Bacillus aneurinilyticus
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-
Manually annotated by BRENDA team
no activity in Bacillus cereus
strain DSM31
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-
Manually annotated by BRENDA team
no activity in Bacillus cereus DSM31
strain DSM31
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-
Manually annotated by BRENDA team
no activity in Bacillus circulans
strain DSM 9
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-
Manually annotated by BRENDA team
no activity in Bacillus licheniformis
strain DSM 13
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-
Manually annotated by BRENDA team
no activity in Bacillus megaterium
strain DSM 32
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-
Manually annotated by BRENDA team
no activity in Bacillus subtilis
strain JH642
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Manually annotated by BRENDA team
no activity in Bacillus subtilis JH642
strain JH642
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-
Manually annotated by BRENDA team
no activity in Bacillus thuringiensis
strain DSM 2046
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Manually annotated by BRENDA team
no activity in Nicotiana tabacum
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-
-
Manually annotated by BRENDA team
no activity in Paenibacillus polymyxa
strain DSM 36
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-
Manually annotated by BRENDA team
strain DSM 33, gene ectC encoded in the ectABC gene cluster
SwissProt
Manually annotated by BRENDA team
formerly Bacillus psychrophilus, strain DSM 3
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-
Manually annotated by BRENDA team
formerl Bacillus pantothenticus, strain DSM 26
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Manually annotated by BRENDA team
formerly Bacillus salexigens, strain DSM 11438
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
show the reaction diagram
D,L-4,5-dihydro-2-methylimidazole-4-carboxylate + H2O
?
show the reaction diagram
L-glutamine
5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate + H2O
show the reaction diagram
L-homoectoine + H2O
?
show the reaction diagram
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r
N4-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
show the reaction diagram
N4-acetyl-L-2,4-diaminobutyrate
2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
show the reaction diagram
N4-acetyl-L-2,4-diaminobutyrate
L-ectoine + H2O
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-N-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
show the reaction diagram
N4-acetyl-L-2,4-diaminobutanoate
L-ectoine + H2O
show the reaction diagram
N4-acetyl-L-2,4-diaminobutyrate
2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
show the reaction diagram
N4-acetyl-L-2,4-diaminobutyrate
L-ectoine + H2O
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
the enzyme is not affected by Mg2+, Ca2+, Mn2+, Li+, K+, and NH4+
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8.4 - 11
4-N-acetyl-L-2,4-diaminobutanoate
0.0287
L-homoectoine
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recombinant enzyme, pH 8.5, 21°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.16
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purified recombinant enzyme, substrate is 4,5-dihydro-2-methylimidazole-4-carboxylate, hydrolytic reaction, pH 8.5, 21°C
0.3
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purified recombinant enzyme, substrate is 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate, hydrolytic reaction, pH 8.5, 21°C
16
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purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0 - 10
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in presence of 0.05 M NaCl
15
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in presence of 0.77 M NaCl
21
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assay at
30
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in presence of 3.0 M NaCl
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0 - 30
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the temperature optimum shifts from 0°C to 30°C with increasing NaCl concentration
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.2 - 4.4
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isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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photosynthetically grown cells
Manually annotated by BRENDA team
additional information
cells are grown in an atmosphere of air-methane 1:1 in 1 M NaCl
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Sphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256)
Sphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15000
SDS-PAGE, calculated
16520
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x * 16520, recombinant His-tagged enzyme, SDS-PAGE
19000
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x * 19000, SDS-PAGE
35000
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gel filtration in presence of 2 M NaCl
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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the enzyme is quite stable below 30°C in presence of 2 M NaCl
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
NaCl stabilizes the enzyme at above 2 M
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native enzyme by ammonium sulfate fractionation, hydrophobic interaction chromatography, ultrafiltration, and hydroxylapatite chromatography, in presence of 1 mM L-2,4-diaminobutanoate and over 2 M NaCl to homogeneity
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recombinant His6-tagged ectoine synthase from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression of His6-tagged ectoine synthase in Escherichia coli strain BL21(DE3)
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functional expression in transgenic Nicotiana tabaccum BY2 cells using the Agrobacterium tumefaciens infection system
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gene ectC encoded in the ectABC gene cluster, functional expression in Escherichia coli
gene ectC located in the singel operon ectABCask, DNA sequence determination and analysis, genetic organization, expression in Escherichia coli strain X1-Blue
gene ectC, DNA and amino acid sequence determination and analysis, genetic mapping and organization, functional expression in Escherichia coli, the recombinant enzyme expression is osmoregulated in response to medium salinity in Escherichia coli
gene ectC, DNA and amino acid sequence determination and analysis, phylogenetic analysis and genetic organization
gene ectC, encoded in the ectABC gene cluster, sequence determination and analysis, phylogenetic analysis, DNA sequence comparison, expression in Escherichia coli
strain TCJAR021, expression in Escherichia coli M15 (pREP4)
the ectABC gene cluster is expressed in Escherichia coli M15 (pREP4) by vector pQE30
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
industry
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