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Literature summary for 4.1.2.43 extracted from
- Bifunctional enzyme fusion of 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase (2007), Appl. Microbiol. Biotechnol., 76, 439-445.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta (DE3) cells, HPS only, or as fusion enzyme with 6-phospho-3-hexuloisomerase | Mycobacterium gastri |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.96 | - |
formaldehyde | purified recombinant enzyme, at 30°C | Mycobacterium gastri |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22000 | - |
2 * 22000, SDS-PAGE | Mycobacterium gastri |
| 45000 | - |
gel filtration | Mycobacterium gastri |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium gastri | - |
- |
- |
| Mycobacterium gastri MB19 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Toyopearl column chromatography butyl-Toyopearl column chromatography | Mycobacterium gastri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 41.1 | - |
cell free extract, recombinant HPS | Mycobacterium gastri |
| 70.9 | - |
after 1.73fold purification, recombinant HPS | Mycobacterium gastri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-ribulose 5-phosphate + formaldehyde | - |
Mycobacterium gastri | D-arabino-hex-3-ulose 6-phosphate | - |
? | |
| D-ribulose 5-phosphate + formaldehyde | - |
Mycobacterium gastri MB19 | D-arabino-hex-3-ulose 6-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 22000, SDS-PAGE | Mycobacterium gastri |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3-hexulose-6-phosphate synthase | - |
Mycobacterium gastri |
| HPS | - |
Mycobacterium gastri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
purified recombinant enzyme | Mycobacterium gastri |
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