Information on EC 4.1.2.43 - 3-hexulose-6-phosphate synthase

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The expected taxonomic range for this enzyme is: Bacteria, Archaea

EC NUMBER
COMMENTARY
4.1.2.43
-
RECOMMENDED NAME
GeneOntology No.
3-hexulose-6-phosphate synthase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
D-arabino-hex-3-ulose 6-phosphate = D-ribulose 5-phosphate + formaldehyde
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
aldol condensation
Q9LBW4
-
aldol condensation
Mycobacterium gastri MB19
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
formaldehyde assimilation II (RuMP Cycle)
-
formaldehyde oxidation I
-
Metabolic pathways
-
Methane metabolism
-
Microbial metabolism in diverse environments
-
Pentose phosphate pathway
-
SYSTEMATIC NAME
IUBMB Comments
D-arabino-hex-3-ulose-6-phosphate formaldehyde-lyase (D-ribulose-5-phosphate-forming)
Requires Mg2+ or Mn2+ for maximal activity [1]. The enzyme is specific for D-ribulose 5-phosphate as substrate as ribose 5-phosphate, xylulose 5-phosphate, allulose 6-phosphate and fructose 6-phosphate cannot act as substrate. In addition to formaldehyde, the enzyme can also use glycolaldehyde and methylglyoxal [7]. This enzyme, along with EC 5.3.1.27, 6-phospho-3-hexuloisomerase, plays a key role in the ribulose-monophosphate cycle of formaldehyde fixation, which is present in many microorganisms that are capable of utilizing C1-compounds [1]. The hyperthermophilic and anaerobic archaeon Pyrococcus horikoshii OT3 constitutively produces a bifunctional enzyme that sequentially catalyses the reactions of this enzyme and EC 5.3.1.27, 6-phospho-3-hexuloisomerase [6]. This enzyme is a member of the orotidine 5'-monophosphate decarboxylase (OMPDC) suprafamily [5].
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3-hexulose phosphate synthase
-
-
-
-
3-hexulose phosphate synthase
-
-
3-hexulose phosphate synthase
-
-
3-hexulose phosphate synthase
Methylomonas sp. M15
-
-
-
3-hexulose phosphate synthase
-
-
3-hexulose phosphate synthase
Mycobacterium gastri MB19
-
-
-
3-hexulose-6-phosphate formaldehyde lyase
Q9F6B7
-
3-hexulose-6-phosphate formaldehyde lyase
Aminomonas aminovorus C2A1
Q9F6B7
-
-
3-hexulose-6-phosphate synthase
Q9F6B7
-
3-hexulose-6-phosphate synthase
Aminomonas aminovorus C2A1
Q9F6B7
-
-
3-hexulose-6-phosphate synthase
-
-
3-hexulose-6-phosphate synthase
-
-
-
3-hexulose-6-phosphate synthase
-
-
3-hexulose-6-phosphate synthase
-
;
-
3-hexulose-6-phosphate synthase
-
-
3-hexulose-6-phosphate synthase
Brevibacillus brevis S1
-
-
-
3-hexulose-6-phosphate synthase
-
-
3-hexulose-6-phosphate synthase
-
-
3-hexulose-6-phosphate synthase
-
-
3-hexulose-6-phosphate synthase
Methylomonas aminofaciens 77a
-
-
-
3-hexulose-6-phosphate synthase
-
-
3-hexulose-6-phosphate synthase
Methylomonas sp. M15
-
-
-
3-hexulose-6-phosphate synthase
-
-
3-hexulose-6-phosphate synthase
-
-
3-hexulose-6-phosphate synthase
Mycobacterium gastri MB19
-
-
-
3-hexulose-6-phosphate synthase
-
-
3-hexulose-6-phosphate synthase
-
-
3-hexulose-6-phosphate synthase
-
3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase activities are expressed constitutively in archea
3-hexulose-6-phosphate synthase
Pyrococcus horikoshii OT-3
-
; 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase activities are expressed constitutively in archea
-
3-hexulose-6-phosphate synthase
-
-
3-hexulose-6-phosphate synthase
-
-
3-hexulose-phosphate synthase
-
-
3-hexulose-phosphate synthase
Methylomonas sp. M15
-
-
-
3-hexulosephosphate synthase
-
-
-
-
3-hexulosephosphate synthase
-
-
3-hexulosephosphate synthase
Acetobacter sp. MB 58
-
;
-
3-hexulosephosphate synthase
-
-
3-hexulosephosphate synthase
-
-
3-hexulosephosphate synthase
Methylomonas aminofaciens 77a
-
;
-
3-hexulosephosphate synthase
-
-
3-hexulosephosphate synthase
Methylomonas sp. GBS, Methylomonas sp. GI33, Methylomonas sp. M15
-
-
-
3-hexulosephosphate synthase
-
-
3-hexulosephosphate synthase
Mycobacterium gastri MB19
-
-
-
3-hexulosephosphate synthase
-
-
3-hexulosephosphate synthase
-
-
3-hexulosephosphate synthase
-
-
-
D-arabino-3-hexulose 6-phosphate formaldehyde lyase
-
-
D-arabino-3-hexulose 6-phosphate formaldehyde lyase
-
-
D-arabino-3-hexulose 6-phosphate formaldehyde lyase
Methylomonas aminofaciens 77a
-
-
-
D-arabino-3-hexulose 6-phosphate formaldehyde-lyase
-
-
-
-
Fae-Hps
-
protein with hexulose-6-phosphate synthase and formaldehyde activating enzyme activities
Fae-Hps
Methanosarcina barkeri Fusaro
-
protein with hexulose-6-phosphate synthase and formaldehyde activating enzyme activities
-
hexose phosphate synthase
-
-
hexulose-6-phosphate synthase
-
-
hexulose-6-phosphate synthase
Methanosarcina barkeri Fusaro
-
-
-
HPS
Acetobacter sp. MB 58
-
;
-
HPS
Acidomonas methanolica MB58
-
-
-
HPS
Aminomonas aminovorus C2A1
Q9F6B7
-
-
HPS
-
3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase activities are expressed constitutively in archea
HPS
-
3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase activities are expressed constitutively in archea
-
HPS
Brevibacillus brevis S1
-
-
-
HPS
-
3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase activities are expressed constitutively in archea
HPS
-
3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase activities are expressed constitutively in archea
HPS
-
3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase activities are expressed constitutively in archea
HPS
Methylomonas aminofaciens 77a
-
;
-
HPS
-
3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase activities are expressed constitutively in archea
HPS
Methylomonas sp. GBS, Methylomonas sp. GI33
-
-
-
HPS
Methylomonas sp. M15
-
3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase activities are expressed constitutively in archea
-
HPS
-
3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase activities are expressed constitutively in archea
HPS
-
3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase activities are expressed constitutively in archea
HPS
Mycobacterium gastri MB19
-
3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase activities are expressed constitutively in archea
-
HPS
Mycobacterium gastri MB19
Q9LBW4
-
-
HPS
-
-
-
HPS
-
-
HPS
Pyrococcus horikoshii OT-3
-
;
-
HPS-aldolase
-
-
HPS-aldolase
Methylomonas sp. M15
-
-
-
HPS-PHI
-
bifunctional enzyme with 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase activities
HPS-PHI
Pyrococcus horikoshii OT-3
-
bifunctional enzyme with 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase activities
-
HPS/PHI
-
bifunctional fusion enzyme
CAS REGISTRY NUMBER
COMMENTARY
55576-36-8
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Acetobacter sp. MB 58
-
-
-
Manually annotated by BRENDA team
strain MB58
-
-
Manually annotated by BRENDA team
Acidomonas methanolica MB58
strain MB58
-
-
Manually annotated by BRENDA team
strain C2A1
UniProt
Manually annotated by BRENDA team
Aminomonas aminovorus C2A1
strain C2A1
UniProt
Manually annotated by BRENDA team
strain MGA3
-
-
Manually annotated by BRENDA team
strain C1
-
-
Manually annotated by BRENDA team
Brevibacillus brevis S1
strain S1
-
-
Manually annotated by BRENDA team
strain Fusaro
-
-
Manually annotated by BRENDA team
Methanosarcina barkeri Fusaro
strain Fusaro
-
-
Manually annotated by BRENDA team
strain KT, obligate methylotroph
-
-
Manually annotated by BRENDA team
formerly Methylococcus capsulatus
-
-
Manually annotated by BRENDA team
Methylomonas aminofaciens 77a
strain 77a
-
-
Manually annotated by BRENDA team
Methylomonas aminofaciens 77a
strain 77a
Uniprot
Manually annotated by BRENDA team
strain GBS; strain GI33; strain M15
-
-
Manually annotated by BRENDA team
strain M15 DSM 580
-
-
Manually annotated by BRENDA team
Methylomonas sp. GBS
strain GBS
-
-
Manually annotated by BRENDA team
Methylomonas sp. GI33
strain GI33
-
-
Manually annotated by BRENDA team
Methylomonas sp. M15
strain M15
-
-
Manually annotated by BRENDA team
Methylomonas sp. M15
strain M15 DSM 580
-
-
Manually annotated by BRENDA team
strain MB19, facultative methylotroph
-
-
Manually annotated by BRENDA team
Mycobacterium gastri MB19
-
-
-
Manually annotated by BRENDA team
Mycobacterium gastri MB19
-
UniProt
Manually annotated by BRENDA team
Mycobacterium gastri MB19
strain MB19
-
-
Manually annotated by BRENDA team
Mycobacterium gastri MB19
strain MB19, facultative methylotroph
-
-
Manually annotated by BRENDA team
strain W6
-
-
Manually annotated by BRENDA team
strain W6
-
-
Manually annotated by BRENDA team
Pyrococcus horikoshii OT-3
strain OT3
-
-
Manually annotated by BRENDA team
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Ca2+
-
49% inhibition at 1 mM
Cu2+
-
24% inhibition at 1 mM
Cu2+
-
45% inhibition with 1 mM Cu2+
Cu2+
-
1 mM Cu2+ is inhibitory, and the effect is partly abolished by Mg2+
D-ribulose 5-phosphate
-
commercial D-ribulose 5-phosphate inhibits the enzyme
EDTA
-
complete inhibition at 10 mM
EDTA
-
the enzyme is completely inhibited in the presence of EDTA, but the activity is restored depending on the amount of MgC12 supplemented
EDTA
-
the enzyme is completely inhibited by the presence of 2 mM EDTA
Glutaraldehyde
-
22% inhibition at 4 mM
glyceraldehyde
-
13% inhibition at 4 mM
glycolaldehyde
-
49% inhibition at 4 mM
glycolic acid
-
glycolic acid inhibits the enzyme competitively with respect to formaldehyde
glyoxylate
-
12% inhibition at 4 mM
Hg2+
-
81% inhibition with 1 mM Hg2+
Ni2+
-
64% inhibition at 1 mM
o-phenanthroline
-
the enzyme is completely inhibited by the presence of 2 mM o-phenanthroline
Pb2+
-
48% inhibition with 1 mM Pb2+
Tiron
-
the enzyme is completely inhibited by the presence of 2 mM tiron
Methylglyoxal
-
26% inhibition at 4 mM
additional information
-
not inhibited by ATP, ADP, AMP, NADH, D-fructose 6-phosphate, and phosphoenolpyruvate
-
additional information
-
not influenced by NaBH4
-
additional information
-
the enzyme is inactivated irreversibly on dialysis against buffer without Mg2+
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.5
8
-
in potassium phosphate buffer
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
40
-
-
purified recombinant enzyme
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.1
-
-
isoelectric focusing
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
in complex with a sulfate ion, sitting drop vapor diffusion method, using 18% (w/v) polyethylene glycol (PEG) 3350 and 0.2 M MgCl2
Q9LBW4
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
8
-
remains stable at pH 6.0-8.0 at 30C for 1 h, enzyme stability decreases at pH values below 6.0
7
8
-
stable in neutral to slightly alkaline solutions
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20C, purified enzyme, at least 1 year, no loss of activity
-
0C to -4C, purified enzyme in the presence of 3.2 M ammonium sulfate, at least 1 year, no loss of activity
-
-80C, purified enzyme, in the presence of 5 mM MgSO4, 5 mM D-ribose 5-phosphate, and 1.75 units/ml phosphoriboisomerase, at least 5 months, no loss of activity
-
-15C, in the presence of 2.5 mM MgCl2, 6 months, remains stable unless repeatedly frozen and thawed
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
H134A
Q9LBW4
the mutation abolishes HPS activity
H134D
Q9LBW4
the mutation abolishes HPS activity
H134K
Q9LBW4
the mutation abolishes HPS activity
H134N
Q9LBW4
the mutation abolishes HPS activity
H134S
Q9LBW4
the mutation abolishes HPS activity
K61A
Q9LBW4
the mutant exhibits 50-90% of the activity of the wild type enzyme
K61D
Q9LBW4
the mutant exhibits 50-90% of the activity of the wild type enzyme
K61H
Q9LBW4
the mutant exhibits 50-90% of the activity of the wild type enzyme
H134A
Mycobacterium gastri MB19
-
the mutation abolishes HPS activity
-
H134D
Mycobacterium gastri MB19
-
the mutation abolishes HPS activity
-
H134K
Mycobacterium gastri MB19
-
the mutation abolishes HPS activity
-
H134N
Mycobacterium gastri MB19
-
the mutation abolishes HPS activity
-
additional information
-
the solvent-tolerant bacterium, Pseudomonas putida S12, is engineered to efficiently utilize methanol and formaldehyde as auxiliary substrates by introducing the hps and phi genes from the thermotolerant methylotrophic bacterium Bacillus brevis, by chemostat culture experiments using glucose and formaldehyde, the hps and phi-expressing strain shows both significantly improved cell mass and growth at higher formaldehyde concentrations than the control strain
additional information
Brevibacillus brevis S1
-
the solvent-tolerant bacterium, Pseudomonas putida S12, is engineered to efficiently utilize methanol and formaldehyde as auxiliary substrates by introducing the hps and phi genes from the thermotolerant methylotrophic bacterium Bacillus brevis, by chemostat culture experiments using glucose and formaldehyde, the hps and phi-expressing strain shows both significantly improved cell mass and growth at higher formaldehyde concentrations than the control strain
-
additional information
-
in order to improve the rate of vanillin degradation by Burkholderia cepacia TM1, the hps and phi genes from Methylomonas aminofaciens 77a are heterologously expressed in strain TM1, the transformant strain constitutively produces active 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase enzymes and the degradation of vanillic acid and the growth yield are significantly improved
additional information
Methylomonas aminofaciens 77a
-
in order to improve the rate of vanillin degradation by Burkholderia cepacia TM1, the hps and phi genes from Methylomonas aminofaciens 77a are heterologously expressed in strain TM1, the transformant strain constitutively produces active 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase enzymes and the degradation of vanillic acid and the growth yield are significantly improved
-
K61S
Q9LBW4
the mutant exhibits 50-90% of the activity of the wild type enzyme
additional information
-
the hps and phi genes from the methylotrophic bacterium Mycobacterium gastri MB19 are introduced into tobacco. Both genes are expressed under the control of the tomato rbcS-3C promoter, and the gene products are targeted to the chloroplasts by artificially added transit peptide sequence, the expression of both genes in plants enhances the tolerance of the transgenic plant to formaldehyde and capability of eliminating environmental formaldehyde
K61A
Mycobacterium gastri MB19
-
the mutant exhibits 50-90% of the activity of the wild type enzyme
-
additional information
Mycobacterium gastri MB19
-
the hps and phi genes from the methylotrophic bacterium Mycobacterium gastri MB19 are introduced into tobacco. Both genes are expressed under the control of the tomato rbcS-3C promoter, and the gene products are targeted to the chloroplasts by artificially added transit peptide sequence, the expression of both genes in plants enhances the tolerance of the transgenic plant to formaldehyde and capability of eliminating environmental formaldehyde
-
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
synthesis
-
enzymatic preparation is suitable for the synthesis of sugars labeled with 13C at specific positions, enzymatic preparation of [1-13C]D-fructose-6-phosphate by using D-ribose-5 phosphate and [1-13C]-formaldehyde as substrates
synthesis
Methylomonas aminofaciens 77a
-
enzymatic preparation is suitable for the synthesis of sugars labeled with 13C at specific positions, enzymatic preparation of [1-13C]D-fructose-6-phosphate by using D-ribose-5 phosphate and [1-13C]-formaldehyde as substrates
-
environmental protection
-
formaldehyde is thought to be the cause of sick house syndrome, transgenic plants harboring the ribulose monophosphate pathway could be useful to improve air pollution in the indoor environment
environmental protection
Mycobacterium gastri MB19
-
formaldehyde is thought to be the cause of sick house syndrome, transgenic plants harboring the ribulose monophosphate pathway could be useful to improve air pollution in the indoor environment
-