Any feedback?
Literature summary for 4.1.1.79 extracted from
- Bifurcated degradative pathway of 3-sulfolactate in Roseovarius nubinhibens ISM via sulfoacetaldehyde acetyltransferase and (S)-cysteate sulfolyase (2009), J. Bacteriol., 191, 5648-5656.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 235000 | - |
native protein with alpha6beta6-structure, determined by gel filtration | Roseovarius nubinhibens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-sulfopyruvate | Roseovarius nubinhibens | - |
2-sulfoacetaldehyde + CO2 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Roseovarius nubinhibens | A3SN11 and A3SN10 | subunit alpha and beta | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by anion exchange chromatography, hydrophobic interaction chromatography and gel filtration | Roseovarius nubinhibens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | ISM strain | Roseovarius nubinhibens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
specific ComDE enzyme activity in extracts from cells grown with sulfolactate is 0.9 mkat/mg protein, 0 mM Tris-HCl (pH 7.5), 1 mM thiamine diphosphate, 1 to 5 mM sulfopyruvate, and protein (0.1-1.0 mg/ml) | Roseovarius nubinhibens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| purified ComDE is stable under an air atmosphere, it can be stored at 4°C for at least a month without loss of activity | Roseovarius nubinhibens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-sulfopyruvate | - |
Roseovarius nubinhibens | 2-sulfoacetaldehyde + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | 2 subunits, determined by SDS-PAGE | Roseovarius nubinhibens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3-sulfopyruvate decarboxylase | - |
Roseovarius nubinhibens |
| ComDE | - |
Roseovarius nubinhibens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | ComDE is involved in sulfonate degradation | Roseovarius nubinhibens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
