Information on EC 4.1.1.79 - sulfopyruvate decarboxylase

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The expected taxonomic range for this enzyme is: Archaea, Bacteria

EC NUMBER
COMMENTARY hide
4.1.1.79
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RECOMMENDED NAME
GeneOntology No.
sulfopyruvate decarboxylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-sulfopyruvate = 2-sulfoacetaldehyde + CO2
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
decarboxylation
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
coenzyme M biosynthesis I
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coenzyme M biosynthesis II
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Methane metabolism
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Microbial metabolism in diverse environments
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sulfolactate degradation II
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coenzyme M biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
3-sulfopyruvate carboxy-lyase (2-sulfoacetaldehyde-forming)
Requires thiamine diphosphate. Does not decarboxylate pyruvate or phosphonopyruvate. The enzyme appears to be oxygen-sensitive.
CAS REGISTRY NUMBER
COMMENTARY hide
303155-97-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
subunitb alpha, P58416, subunit beta
SwissProt
Manually annotated by BRENDA team
subunit alpha and beta
A3SN11 and A3SN10
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
disruption of the gene comE by transposon mutagenesis results in a partial coenzyme M auxotroph, which grows poorly in the absence of coenzyme M and retains less than 3% of the wild type level of coenzyme M biosynthesis. Upon coenzyme M addition, normal growth of the mutant is restored. Complementation of the mutation with the wild type comE gene in trans restores full growth in the absence of coenzyme M
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-sulfopyruvate
2-sulfoacetaldehyde + CO2
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-sulfopyruvate
2-sulfoacetaldehyde + CO2
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
thiamine diphosphate
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
A3SN11 and A3SN10
ISM strain
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
17000
alpha6beta6, 6 * 17000 + 6 * 23000, SDS-PAGE
23000
alpha6beta6, 6 * 17000 + 6 * 23000, SDS-PAGE
42300
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calculated from amino acid sequence
43000
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refolded MA3298 protein, SDS-PAGE
103000
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NusA-tagged MA3298 protein, SDS-PAGE
235000
A3SN11 and A3SN10
native protein with alpha6beta6-structure, determined by gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dodecamer
alpha6beta6, 6 * 17000 + 6 * 23000, SDS-PAGE
heterodimer
A3SN11 and A3SN10
2 subunits, determined by SDS-PAGE
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
inactivation by oxygen at 80°C and reactivation by reduction with dithionite
644226
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
purified ComDE is stable under an air atmosphere, it can be stored at 4°C for at least a month without loss of activity
A3SN11 and A3SN10
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by anion exchange chromatography, hydrophobic interaction chromatography and gel filtration
A3SN11 and A3SN10
Ni2+ affinity column chromatography, gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) RIL cells containing vector pDG121
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expression in Escherichia coli
Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Show AA Sequence (172 entries)
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