Application | Comment | Organism |
---|---|---|
synthesis | expression and purification of a monomeric, stable and active enzyme in the absence of vinylpyruvate hydratase | Pseudomonas putida |
Cloned (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Crystallization (Comment) | Organism |
---|---|
crystal structures for NahK in apo form and bonded with substrate analogues. A lid domain may be implicated in substrate binding | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0049 | - |
(3E)-2-oxohex-3-enedioate | 25°C, pH 8.0 | Pseudomonas putida | |
0.0199 | - |
(3E)-2-oxohex-3-enedioate | 25°C, pH 7.4 | Pseudomonas putida | |
0.0222 | - |
(3E)-2-oxohex-3-enedioate | 25°C, pH 7.0 | Pseudomonas putida | |
0.0517 | - |
(3E)-2-oxohex-3-enedioate | 25°C, pH 6.0 | Pseudomonas putida | |
0.0647 | - |
(3E)-2-oxohex-3-enedioate | 25°C, pH 6.5 | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | assay in presence of 5 mM Mg2+ | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | 30000 | gel filtration | Pseudomonas putida |
26000 | - |
dynamic light scattering | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | Q1XGK3 | - |
- |
Pseudomonas putida G7 | Q1XGK3 | - |
- |
Purification (Comment) | Organism |
---|---|
expression and purification of a monomeric, stable and active 4-OD in the absence of vinylpyruvate hydratase | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3E)-2-oxohex-3-enedioate | - |
Pseudomonas putida | 2-oxopent-4-enoate + CO2 | - |
? | |
(3E)-2-oxohex-3-enedioate | - |
Pseudomonas putida G7 | 2-oxopent-4-enoate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 28400, calculated from sequence | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
NahK | - |
Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21.8 | - |
(3E)-2-oxohex-3-enedioate | 25°C, pH 8.0 | Pseudomonas putida | |
22.5 | - |
(3E)-2-oxohex-3-enedioate | 25°C, pH 6.0 | Pseudomonas putida | |
98.8 | - |
(3E)-2-oxohex-3-enedioate | 25°C, pH 7.4 | Pseudomonas putida | |
104.2 | - |
(3E)-2-oxohex-3-enedioate | 25°C, pH 7.0 | Pseudomonas putida | |
108.4 | - |
(3E)-2-oxohex-3-enedioate | 25°C, pH 6.5 | Pseudomonas putida |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
440 | - |
(3E)-2-oxohex-3-enedioate | 25°C, pH 6.0 | Pseudomonas putida | |
1700 | - |
(3E)-2-oxohex-3-enedioate | 25°C, pH 6.5 | Pseudomonas putida | |
4500 | - |
(3E)-2-oxohex-3-enedioate | 25°C, pH 8.0 | Pseudomonas putida | |
4700 | - |
(3E)-2-oxohex-3-enedioate | 25°C, pH 7.0 | Pseudomonas putida | |
5000 | - |
(3E)-2-oxohex-3-enedioate | 25°C, pH 7.4 | Pseudomonas putida |