Information on EC 4.1.1.77 - 2-oxo-3-hexenedioate decarboxylase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.1.1.77
-
RECOMMENDED NAME
GeneOntology No.
2-oxo-3-hexenedioate decarboxylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(3E)-2-oxohex-3-enedioate = 2-oxopent-4-enoate + CO2
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
decarboxylation
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
2,3-dihydroxybenzoate degradation
-
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2-amino-3-carboxymuconate semialdehyde degradation to 2-oxopentenoate
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2-aminophenol degradation
-
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2-nitrobenzoate degradation I
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3-chlorocatechol degradation III (meta pathway)
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-
4-amino-3-hydroxybenzoate degradation
-
-
4-chloronitrobenzene degradation
-
-
4-nitrotoluene degradation II
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Benzoate degradation
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catechol degradation to 2-oxopent-4-enoate II
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Dioxin degradation
-
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Metabolic pathways
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Microbial metabolism in diverse environments
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orthanilate degradation
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protocatechuate degradation III (para-cleavage pathway)
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Xylene degradation
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phenol degradation
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tryptophan metabolism
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SYSTEMATIC NAME
IUBMB Comments
(3E)-2-oxohex-3-enedioate carboxy-lyase (2-oxopent-4-enoate-forming)
Involved in the meta-cleavage pathway for the degradation of phenols, modified phenols and catechols. The enzyme has been reported to accept multiple tautomeric forms [1-4]. However, careful analysis of the stability of the different tautomers, as well as characterization of the enzyme that produces its substrate, EC 5.3.2.6, 2-hydroxymuconate tautomerase, showed that the actual substrate for the enzyme is (3E)-2-oxohex-3-enedioate [4].
CAS REGISTRY NUMBER
COMMENTARY hide
37325-55-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain 10d
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Manually annotated by BRENDA team
strain 10d
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-
Manually annotated by BRENDA team
basonym Alcaligenes eutrophus; strain 345; strain RA1012
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-
Manually annotated by BRENDA team
strain 345
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-
Manually annotated by BRENDA team
strain RA1012
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-
Manually annotated by BRENDA team
strain CF600
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-
Manually annotated by BRENDA team
strain CF600
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-
Manually annotated by BRENDA team
strain PKO1
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-
Manually annotated by BRENDA team
strain PKO1
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-oxo-3-hexenedioate
2-oxopent-4-enoate + CO2
show the reaction diagram
-
4-OD and vinylpyruvate hydratase form a complex, 2-hydroxy-2,4-pentadienoate is the product of 4-OD and 2-oxo-4-pentenoate results from a vinylpyruvate hydratase-catalyzed process
-
?
4-Oxalocrotonate
2-Oxopent-4-enoate + CO2
show the reaction diagram
4-Oxalocrotonate
?
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-Oxalocrotonate
2-Oxopent-4-enoate + CO2
show the reaction diagram
4-Oxalocrotonate
?
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
required
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2E)-2-fluorohex-2-en-4-ynoic acid
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-
(2E)-2-fluoropent-2-en-4-ynoic acid
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(2E)-2-fluoropenta-2,4-dienoic acid
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(2E,4E)-2-fluorohexa-2,4-dienedioic acid
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(2E,4E)-2-fluorohexa-2,4-dienoic acid
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(2Z)-2-fluorohex-2-en-4-ynedioic acid
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(2Z)-2-fluoropent-2-en-4-ynoic acid
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(2Z)-2-fluoropenta-2,4-dienoic acid
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(2Z,4E)-2-fluorohexa-2,4-dienoic acid
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CoCl2
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1 mM, 47% remaining activity
FeSO4
-
1 mM, 23% remaining activity
iodoacetic acid
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1 mM, 64% remaining activity
K3Fe(CN)6
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1 mM, 36% remaining activity
MnSO4
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1 mM, 9% remaining activity
N-Methylmaleimide
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1 mM, 58% remaining activity
p-chloromercuribenzoic acid
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1 mM, 54% remaining activity
Tiron
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1 mM, 59% remaining activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
68
2-oxo-3-hexenedioate
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pH 7.3
0.006 - 0.047
4-oxalocrotonate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
68
2-oxo-3-hexenedioate
Pseudomonas putida
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-
0.02 - 68
4-oxalocrotonate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0068
(2E)-2-fluorohex-2-en-4-ynoic acid
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pH 7.3, 22C
0.014
(2E)-2-fluoropent-2-en-4-ynoic acid
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pH 7.3, 22C
0.00011
(2E)-2-fluoropenta-2,4-dienoic acid
-
pH 7.3, 22C
0.045
(2E,4E)-2-fluorohexa-2,4-dienedioic acid
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pH 7.3, 22C
0.0022
(2E,4E)-2-fluorohexa-2,4-dienoic acid
-
pH 7.3, 22C
0.0003
(2Z)-2-fluorohex-2-en-4-ynedioic acid
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pH 7.3, 22C
0.05
(2Z)-2-fluoropent-2-en-4-ynoic acid
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pH 7.3, 22C
0.00034
(2Z)-2-fluoropenta-2,4-dienoic acid
-
pH 7.3, 22C
0.0085
(2Z,4E)-2-fluorohexa-2,4-dienoic acid
-
pH 7.3, 22C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.05
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cell extract
18
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after 360fold purification
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28900
calculated from the deduced amino acid sequences
29000
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SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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enzyme forms a complex with vinylpyruvate hydratase, which is only active when stabilized in this complex
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
complex with vinylpyruvate hydratase, homogenetiy
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DE52 cellulose column chromatography and DEAE-Cellulofine A800 column chromatography, DEAE-Toyopearl column chromatography, streptomycin sulfate precipitation, and ammonium sulfate precipitation
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E109Q
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7fold increase in Km-value, 2270fold decrease in kcat, no influence of mutation to vinylpyruvate hydratase activity
E111Q
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Km-value comparable to wild-type, 3400fold decrease in kcat no influence of mutation to vinylpyruvate hydratase activity
Show AA Sequence (314 entries)
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