Cloned (Comment) | Organism |
---|---|
gene gad, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant overexpression in Escherichia coli strain BL21(DE3), real-time PCR enzyme expression analysis | Synechocystis sp. PCC 6803 |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of an enzyme deletion strain, DELTAgad, transcription levels of genes involved in nitrogen metabolism are upregulated in the mutant strain | Synechocystis sp. PCC 6803 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
19.6 | - |
L-glutamate | pH 6.0, 30°C, recombinant enzyme | Synechocystis sp. PCC 6803 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
recombinant enzyme, gel filtration | Synechocystis sp. PCC 6803 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | Synechocystis sp. PCC 6803 | - |
4-aminobutanoate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. PCC 6803 | P73043 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | - |
Synechocystis sp. PCC 6803 | 4-aminobutanoate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 53000, recombinant enzyme, SDS-PAGE | Synechocystis sp. PCC 6803 |
Synonyms | Comment | Organism |
---|---|---|
GAD | - |
Synechocystis sp. PCC 6803 |
sll1641 | ORF name | Synechocystis sp. PCC 6803 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
recombinant enzyme | Synechocystis sp. PCC 6803 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | activity range, recombinant enzyme, profile overview | Synechocystis sp. PCC 6803 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
100.7 | - |
L-glutamate | pH 6.0, 30°C, recombinant enzyme | Synechocystis sp. PCC 6803 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | 6 | recombinant enzyme | Synechocystis sp. PCC 6803 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 7 | narrow activity range, recombinant enzyme, profile overview | Synechocystis sp. PCC 6803 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on, best at 0.04 mM | Synechocystis sp. PCC 6803 |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Synechocystis sp. PCC 6803 | sequence calculation | - |
5.1 |
General Information | Comment | Organism |
---|---|---|
malfunction | transcription levels of genes involved in nitrogen metabolism are upregulated in the DELTAgad enzyme deletion strain. The mutant shows approximately 4 and 8fold increases in the transcript levels of kgd and gabdh encoding a novel 2-oxoglutarate decarboxylase and gamma-aminobutanal dehydrogenase, respectively. Phenotype, overview. In Synechocystis lacking a functional GAD, the gamma-aminobutanal dehydrogenase might serve as an alternative catalytic pathway for GABA synthesis | Synechocystis sp. PCC 6803 |
physiological function | glutamate decarboxylase (GAD) is the enzyme responsible for the synthesis of gamma-aminobutyric acid (GABA) in Synechocystis sp. PCC6803 | Synechocystis sp. PCC 6803 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.1 | - |
L-glutamate | pH 6.0, 30°C, recombinant enzyme | Synechocystis sp. PCC 6803 |