Any feedback?
Literature summary for 3.8.1.9 extracted from
- Insights into the molecular mechanism of dehalogenation catalyzed by D-2-haloacid dehalogenase from crystal structures (2018), Sci. Rep., 8, 1454 .
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystals are obtained by hanging-drop vapour diffusion method. The enzyme is crystallized in the presence of the substrate D-2-chloropropionate at pH 5.5-6.4 and 4°C. 2.64 A resolution | Pseudomonas putida |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D205E | activity with D-2-chloropropionate is 10.1% compared to activity of the wild-type enzyme. The kcat value of wild-type enzyme is 13fold higher compared to mutant enzyme | Pseudomonas putida |
| D205N | no activity with D-2-chloropropionate | Pseudomonas putida |
| F281A | activity with D-2-chloropropionate is 54.7% compared to activity of the wild-type enzyme | Pseudomonas putida |
| G50A | activity with D-2-chloropropionate is 5.1% compared to activity of the wild-type enzyme | Pseudomonas putida |
| I52G | activity with D-2-chloropropionate is 1.5% compared to activity of the wild-type enzyme | Pseudomonas putida |
| L285I | activity with D-2-chloropropionate is 24.4% compared to activity of the wild-type enzyme | Pseudomonas putida |
| L288I | activity with D-2-chloropropionate is 37.2% compared to activity of the wild-type enzyme | Pseudomonas putida |
| M284C | activity with D-2-chloropropionate is 7.0% compared to activity of the wild-type enzyme | Pseudomonas putida |
| N131D | activity with D-2-chloropropionate is 4.4% compared to activity of the wild-type enzyme | Pseudomonas putida |
| N203A | activity with D-2-chloropropionate is 1.2% compared to activity of the wild-type enzyme. The kcat value of wild-type enzyme is 355fold higher compared to mutant enzyme | Pseudomonas putida |
| N203S | activity with D-2-chloropropionate is 0.4% compared to activity of the wild-type enzyme | Pseudomonas putida |
| S204A | activity with D-2-chloropropionate is 4.9% compared to activity of the wild-type enzyme | Pseudomonas putida |
| S204T | activity with D-2-chloropropionate is 41.8% compared to activity of the wild-type enzyme | Pseudomonas putida |
| V51F | activity with D-2-chloropropionate is 36.6% compared to activity of the wild-type enzyme. The kcat value of wild-type enzyme is 4fold higher compared to mutant enzyme | Pseudomonas putida |
| W48A | activity with D-2-chloropropionate is 0.2% compared to activity of the wild-type enzyme | Pseudomonas putida |
| Y134F | activity with D-2-chloropropionate is 0.1% compared to activity of the wild-type enzyme | Pseudomonas putida |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.94 | - |
D-2-chloropropionate | wild-type enzyme, pH 10.0, 30°C | Pseudomonas putida |  |
| 2.9 | - |
D-2-chloropropionate | mutant enzyme V51F, pH 10.0, 30°C | Pseudomonas putida | |
| 4.13 | - |
D-2-chloropropionate | mutant enzyme D205E, pH 10.0, 30°C | Pseudomonas putida | |
| 12 | - |
D-2-chloropropionate | mutant enzyme N203A, pH 10.0, 30°C | Pseudomonas putida | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | Q52086 | - |
- |
| Pseudomonas putida AJ1/23 | Q52086 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-2-chloropropionate + H2O | - |
Pseudomonas putida | L-2-hydroxypropionate + chloride | - |
? | |
| D-2-chloropropionate + H2O | - |
Pseudomonas putida AJ1/23 | L-2-hydroxypropionate + chloride | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | - |
Pseudomonas putida |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-2-haloacid dehalogenase | - |
Pseudomonas putida |
| D-DEX | - |
Pseudomonas putida |
| HadD AJ1 | - |
Pseudomonas putida |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.59 | - |
D-2-chloropropionate | mutant enzyme N203A, pH 10.0, 30°C | Pseudomonas putida | |
| 12.6 | - |
D-2-chloropropionate | mutant enzyme V51F, pH 10.0, 30°C | Pseudomonas putida | |
| 16.35 | - |
D-2-chloropropionate | wild-type enzyme, pH 10.0, 30°C | Pseudomonas putida | |
| 55 | - |
D-2-chloropropionate | mutant enzyme D205E, pH 10.0, 30°C | Pseudomonas putida | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
