Information on EC 3.8.1.9 - (R)-2-haloacid dehalogenase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.8.1.9
-
RECOMMENDED NAME
GeneOntology No.
(R)-2-haloacid dehalogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(R)-2-haloacid + H2O = (S)-2-hydroxyacid + halide
show the reaction diagram
SYSTEMATIC NAME
IUBMB Comments
(R)-2-haloacid halidohydrolase
Acts on acids of short chain lengths, C2 to C4, with inversion of configuration at C-2. [See also EC 3.8.1.2 (S)-2-haloacid dehalogenase, EC 3.8.1.10 2-haloacid dehalogenase (configuration-inverting) and EC 3.8.1.11 2-haloacid dehalogenase (configuration-retaining)]
CAS REGISTRY NUMBER
COMMENTARY hide
119345-29-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
from the intertidal coast of the Chinese Yellow Sea near Dalian City
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-
Manually annotated by BRENDA team
strain AJ1/23
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-
Manually annotated by BRENDA team
strain AJ1/23
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R)-2-chloropropionic acid + H2O
(S)-2-hydroxypropionic acid + chloride
show the reaction diagram
(R)-2-haloacid + H2O
(S)-2-hydroxyacid + halide
show the reaction diagram
2,2-dichloropropionate + H2O
?
show the reaction diagram
3-chloropropionate + H2O
?
show the reaction diagram
-
-
-
?
3-chloropropionate + H2O
hydroxypropionate + chloride
show the reaction diagram
D-2-bromopropionate + H2O
L-2-hydroxypropionate + bromide
show the reaction diagram
-
-
-
-
?
D-2-chloropropionate + H2O
?
show the reaction diagram
-
-
-
?
D-2-chloropropionate + H2O
L-2-hydroxypropionate + chloride
show the reaction diagram
-
-
-
-
?
dibromoacetate + H2O
?
show the reaction diagram
-
-
-
?
dichloroacetate + H2O
?
show the reaction diagram
DL-2,3-dichloropropionate + H2O
?
show the reaction diagram
DL-2-bromopropionate + H2O
lactate + HBr
show the reaction diagram
DL-2-chloropropionate + H2O
lactate + HCl
show the reaction diagram
L-2-bromopropionate + H2O
?
show the reaction diagram
-
-
-
?
L-2-chloropropionate + H2O
?
show the reaction diagram
monobromoacetate + H2O
2-hydroxyacetate + bromide
show the reaction diagram
-
-
-
?
monobromoacetate + H2O
glycolate + HBr
show the reaction diagram
monobromoacetate + H2O
hydroxyacetate + bromide
show the reaction diagram
-
-
-
-
?
monochloroacetate + H2O
2-hydroxyacetate + chloride
show the reaction diagram
-
-
-
?
monochloroacetate + H2O
glycolate + HCl
show the reaction diagram
monochloroacetate + H2O
hydroxyacetate + chloride
show the reaction diagram
-
-
-
-
?
tribromoacetate + H2O
?
show the reaction diagram
-
-
-
?
trichloroacetate + H2O
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(R)-2-chloropropionic acid + H2O
(S)-2-hydroxypropionic acid + chloride
show the reaction diagram
-
-
-
-
?
(R)-2-haloacid + H2O
(S)-2-hydroxyacid + halide
show the reaction diagram
DL-2,3-dichloropropionate + H2O
?
show the reaction diagram
Q8KLS9
-
-
-
?
monobromoacetate + H2O
2-hydroxyacetate + bromide
show the reaction diagram
Q8KLS9
-
-
-
?
monochloroacetate + H2O
2-hydroxyacetate + chloride
show the reaction diagram
Q8KLS9
-
-
-
?
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
thiol reagents
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not
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.9
D-2-chloropropionate
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1.99
DL-2-bromopropionate
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pH 7.5, D-2-haloacid dehalogenase subtype
27.5
Monochloroacetate
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pH 7.5, D-2-haloacid dehalogenase subtype
additional information
additional information
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kinetic studies
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.12
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substrate (R)-2-chloropropionic acid, pH 7.3, 30°C
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.3
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assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
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chromatofocusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
-
the growth of DEH 138 strain culture is not affected by salinity of 0-15%. Growth on 1,2-dichloroethane, 3-chloro-1,2-propanediol, 2,2-dichloropropionic acid, and 2,4,6-trichlorophenol medium
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
29383
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2 * 29000, SDS-PAGE, 2 * 29383, calculated from amino acid sequence
33601
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4 * 33601
53000
gel filtration
60000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
additional information
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
strain AJ1/23, D-2-haloacid dehalogenase subtype, activity loss above 40°C, rapid protein denaturation and precipitation at 56°C
50
-
12 min, about 90% loss of activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant wild-type and mutant enzymes from Escherichia coli
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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gene dehD, sequence comparisons, recombinant expression of wild-type and mutant enzymes in Escherichia coli
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phylogenetic analysis
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
R134A
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site-directed mutagenesis, the DehD mutant variant demonstrates increased propensity for binding haloalkanoic acid and is non-stereospecific towards halogenated substrates
Y135A
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site-directed mutagenesis, the DehD mutant variant demonstrates increased propensity for binding haloalkanoic acid and is non-stereospecific towards halogenated substrates
R134A
-
site-directed mutagenesis, the DehD mutant variant demonstrates increased propensity for binding haloalkanoic acid and is non-stereospecific towards halogenated substrates
-
Y135A
-
site-directed mutagenesis, the DehD mutant variant demonstrates increased propensity for binding haloalkanoic acid and is non-stereospecific towards halogenated substrates
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information