Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) pLysE cells | Mus musculus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method, using 5% (w/v) polyethylene glycol 3350 and 100 mM MES (pH 6.0) | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
apo-form, Guinier analysis | Mus musculus |
50000 | - |
ADP-bound form, Guinier analysis | Mus musculus |
148000 | - |
ATP-bound form, Guinier analysis | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | P46467 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, DEAE Sepharose column chromatography, and Superdex 75 gel filtration | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Mus musculus | ADP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | addition of ATP but not ADP increases the oligomerized population of SKD1 | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
SKD1/VPS4B | - |
Mus musculus |