Information on EC 3.6.4.6 - vesicle-fusing ATPase

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The expected taxonomic range for this enzyme is: Eukaryota, Archaea

EC NUMBER
COMMENTARY
3.6.4.6
-
RECOMMENDED NAME
GeneOntology No.
vesicle-fusing ATPase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ATP + H2O = ADP + phosphate
show the reaction diagram
-
-
-
-
ATP + H2O = ADP + phosphate
show the reaction diagram
large family of ATP-hydrolysing enzymes involved in the heterotypic fusion of membrane vesicles with target membranes and the homotypic fusion of various membrane compartments. They belong to the AAA-type (ATPase associated with a variety of cell activities) ATPase superfamily. They include peroxin, which apparently is involved in Zellweger 's syndrome.
-
ATP + H2O = ADP + phosphate
show the reaction diagram
large family of ATP-hydrolysing enzymes involved in the heterotypic fusion of membrane vesicles with target membranes and the homotypic fusion of various membrane compartments. They belong to the AAA-type (ATPase associated with a variety of cell activities) ATPase superfamily. They include peroxin, which apparently is involved in Zellweger 's syndrome.
-
ATP + H2O = ADP + phosphate
show the reaction diagram
large family of ATP-hydrolysing enzymes involved in the heterotypic fusion of membrane vesicles with target membranes and the homotypic fusion of various membrane compartments. They belong to the AAA-type (ATPase associated with a variety of cell activities) ATPase superfamily. They include peroxin, which apparently is involved in Zellweger 's syndrome.
-
ATP + H2O = ADP + phosphate
show the reaction diagram
large family of ATP-hydrolysing enzymes involved in the heterotypic fusion of membrane vesicles with target membranes and the homotypic fusion of various membrane compartments. They belong to the AAA-type (ATPase associated with a variety of cell activities) ATPase superfamily. They include peroxin, which apparently is involved in Zellweger 's syndrome.
-
ATP + H2O = ADP + phosphate
show the reaction diagram
large family of ATP-hydrolysing enzymes involved in the heterotypic fusion of membrane vesicles with target membranes and the homotypic fusion of various membrane compartments. They belong to the AAA-type (ATPase associated with a variety of cell activities) ATPase superfamily. They include peroxin, which apparently is involved in Zellweger 's syndrome.
-
ATP + H2O = ADP + phosphate
show the reaction diagram
large family of ATP-hydrolysing enzymes involved in the heterotypic fusion of membrane vesicles with target membranes and the homotypic fusion of various membrane compartments. They belong to the AAA-type (ATPase associated with a variety of cell activities) ATPase superfamily. They include peroxin, which apparently is involved in Zellweger 's syndrome.
-
ATP + H2O = ADP + phosphate
show the reaction diagram
large family of ATP-hydrolysing enzymes involved in the heterotypic fusion of membrane vesicles with target membranes and the homotypic fusion of various membrane compartments. They belong to the AAA-type (ATPase associated with a variety of cell activities) ATPase superfamily. They include peroxin, which apparently is involved in Zellweger 's syndrome.
-
ATP + H2O = ADP + phosphate
show the reaction diagram
large family of ATP-hydrolysing enzymes involved in the heterotypic fusion of membrane vesicles with target membranes and the homotypic fusion of various membrane compartments. They belong to the AAA-type (ATPase associated with a variety of cell activities) ATPase superfamily. They include peroxin, which apparently is involved in Zellweger 's syndrome.
-
ATP + H2O = ADP + phosphate
show the reaction diagram
large family of ATP-hydrolysing enzymes involved in the heterotypic fusion of membrane vesicles with target membranes and the homotypic fusion of various membrane compartments. They belong to the AAA-type (ATPase associated with a variety of cell activities) ATPase superfamily. They include peroxin, which apparently is involved in Zellweger 's syndrome.
-
ATP + H2O = ADP + phosphate
show the reaction diagram
Vps4 disassembles the ESCRT-III protein polymer, thereby changing the morphology of the underlying membrane
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
hydrolysis of phosphoric ester
P46461
;
hydrolysis of phosphoric ester
-
-
hydrolysis of phosphoric ester
-
-
hydrolysis of phosphoric ester
-
-
hydrolysis of phosphoric ester
-
-
hydrolysis of phosphoric ester
-
-
hydrolysis of phosphoric ester
-
-
hydrolysis of phosphoric ester
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP phosphohydrolase (vesicle-fusing)
A large family of ATP-hydrolysing enzymes involved in the heterotypic fusion of membrane vesicles with target membranes and the homotypic fusion of various membrane compartments. They belong to the AAA-type (_A_TPase _a_ssociated with a variety of cell _a_ctivities) ATPase superfamily. They include peroxin, which apparently is involved in Zellweger's syndrome.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
AAA ATPase Vps4
-
-
AAA ATPase Vps4
Q97ZJ7
-
AAA ATPase Vps4
Q97ZJ7
-
-
AAA-ATPase
P52917
-
ATP phosphohydrolase
-
-
ATP phosphohydrolase
-
-
ATPase N-ethylmaleimide sensitive factor
-
-
dNSF1
-
isoform 1
dNSF1
P46461
isoform 2
Hez-NSF
-
-
HsPEX1
-
-
HsPex1p
-
-
membrane deformation AAA ATPase
-
-
N-ethyl maleimide sensitive factor
-
-
N-ethylmaleimide sensitive factor
-
-
N-ethylmaleimide sensitive factor
-, P46461
-
N-ethylmaleimide sensitive factor
-
-
N-ethylmaleimide sensitive factor
-
-
N-ethylmaleimide sensitive factor
-
-
N-ethylmaleimide sensitive fusion protein
-
-
-
-
N-ethylmaleimide sensitive fusion protein
-
-
N-ethylmaleimide sensitive-factor
-
-
N-ethylmaleimide sensitive-factor
-
-
N-ethylmaleimide-sensitive factor
-
-
N-ethylmaleimide-sensitive factor
-
-
N-ethylmaleimide-sensitive factor
-
-
N-ethylmaleimide-sensitive fusion protein
-
-
N-ethylmaleimide-sensitive fusion protein
-
-
N-ethylmaleimide-sensitive fusion protein
-
-
N-ethylmaleimide-sensitive fusion protein
-
-
N-ethylmeleimide-sensitive factor
-
-
NEM-sensitive fusion protein
-
-
-
-
NEM-sensitive fusion protein
-
-
NSF
-
-
NSF
-
-
NSF protein
-
N-ethyl-maleimide-sensitive fusion protein
NSF protein
-
-
NSF protein
-
N-ethyl-maleimide-sensitive fusion protein
NSF-1
-
there are two NSF-1 isoforms, which are expressed in distinct tissues through two separate promoters
p97
protozoa
-
-
p97/VCP/Cdc48p
-
-
p97/VCP/Cdc48p homologue
-
-
p97ATPase
-
-
peptide p97
P46462
essential chaperone in endoplasmic reticulum-associated degradation and organelle biogenesis, contains two ATPases associated with various cellular activities domains (D1 and D2)
Pex1p
-
-
Pex6p
-
-
rat Pex6p
-
-
SEC18 gene product
-
-
SEC18p
-
-
SKD1/VPS4B
P46467
-
SKD2 protein
-
-
-
-
Sso0909
Q97ZJ7
locus name
Sso0909
Q97ZJ7
locus name
-
SsoVps4
Q97ZJ7
-
SsoVps4
Q97ZJ7
-
-
suppresor of potassium transport growth defect
-
-
vacular protein sorting 4 B
-
-
vacuolar protein sorting 4
-
-
vacuolar protein sorting 4
Q97ZJ7
-
vacuolar protein sorting 4
Q97ZJ7
-
-
vacuolar protein sorting 4 protein
P52917
-
vasolin-containing protein
P46462
-
VCP
P46462
-
vesicle-fusing ATPase
-
-
vesicle-fusing ATPase
-
-
Vesicular-fusion protein NSF
-
-
-
-
Vps 4B
-
-
VPS4 gene product
-
-
Vps4p
-
a type I ATPase associated with a variety of cellular activities ATPase
Vps4p AAA ATPase
-
-
CAS REGISTRY NUMBER
COMMENTARY
9000-83-3
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
long-tailed hamster
-
-
Manually annotated by BRENDA team
HeLa cells; human embryonic kidney HEK293 cells; human epidermoid carcinoma A-431 cells
-
-
Manually annotated by BRENDA team
protozoa
-
-
-
Manually annotated by BRENDA team
rat; rat hepatoma H-4-II-E cells
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
-
inhibition of p97, but not NSF ATPase can be associated with ER/Golgi disruption and apoptosis in alphaSNAP-depleted epithelial cells. AlphaSNAP knockdown does not affect p97 expression, it perturbes a balance between key p97-binding partners. Specifically, expression of syntaxins 5 and 18 are significantly decreased
physiological function
-
Vps4 functions together with the protein complex ESCRT-III in membrane fission
physiological function
Q97ZJ7
the enzyme provides energy for the ESCRT (endosomal sorting complexes required for transport) pathway
physiological function
-
the enzyme provides energy for the ESCRT (endosomal sorting complexes required for transport) pathway
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
P54351
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
P46461
-
-
-
ir
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
ir
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
ir
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
ir
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
ir
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
ir
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
ir
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
ir
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
ir
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
protozoa
-
-
-
-
ir
ATP + H2O
ADP + phosphate
show the reaction diagram
P52917
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
P46467
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
P46462
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
dNSF-1 is involved in disassembling SNARE-complexes after fusion and before endocytosis
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
involved in membrane transport through endosome
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
a central function of Helar-NSF in developmental process through regulating neurohormonr release
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
ATPase N-ethylmaleimide sensitive factor may be a common feature in the triggering/regulation of membrane merging during mammalian acrosome reaction
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
coordinated action of NSF and PKC regulates GABAB receptor signaling efficacy
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
essential requirement for NSF in the organization of myelinated axons
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
N-ethylmaleimide-sensitive factor is required for the synaptic incorporation and removal of 2-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors during cerebellar long-term depression
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
NSF is a critical regulator of leukocyte trafficking in vivo
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
NSF is important in vesicular trafficking, but neural expression of a dominant negative form of NSF2 induces an unexpected overgrowth of the DRosophila larval neuromuscular synapse
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
PEX1 is indispensable for biogenesis and maintenance of the peroxisome
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
Pex1p possesses two distinct oligomeric forms, a homo-oligomer in the cytosol and a hetero-oligomer on peroxisome membranes, possibly playing distinct functions in peroxisome biogenesis
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
the enzyme plays a crucial role in the fusion of vesicles with target membranes. NSF-SNAP complexes may be involved in the disassembly of coiled-coil-mediated interactions underlying not only SNARE complexes but also other forms of stable oligomers, and the C-terminal membrane anchoring of NSF-SNAP targets may be mechanistically involved in the dissociation process
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
the enzyme plays an essential role in intracellular membrane transport events
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
the VCP/Cdc48p homologues C41C4.8 and C06A1.1, AAA chaperones, may play a protective role in polyglutamine aggregation
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
Vsp4 is an AAA ATPase which plays a key role in sorting of multivesicular bodies and facilitates transport through endosomes. The beta-domain of Vsp4p plays a critical role in the formation of a Vsp4p ATPase complex that is functional in vivo. The formation of the latter may be a prerequisite for interaction with Vta1p
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
Vps4 disassembles the ESCRT-III protein polymer, thereby changing the morphology of the underlying membrane. The disassembly reaction causes the ESCRT-III subunits to regain the monomeric conformational state, which is the high-energy state that is poised to reassemble again into the ESCRT-III oligomer for subsequent rounds of membrane scission. When assembled, Vps4 progresses through many ATP hydrolysis cycles without dissociation, Vps4 disassembles the ESCRT-III protein polymer, thereby changing the morphology of the underlying membrane
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
P54351
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
P46461
-
-
-
ir
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
ir
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
ir
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
ir
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
ir
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
ir
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
ir
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
ir
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
ir
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
protozoa
-
-
-
-
ir
ATP + H2O
ADP + phosphate
show the reaction diagram
-
a central function of Helar-NSF in developmental process through regulating neurohormonr release
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
ATPase N-ethylmaleimide sensitive factor may be a common feature in the triggering/regulation of membrane merging during mammalian acrosome reaction
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
coordinated action of NSF and PKC regulates GABAB receptor signaling efficacy
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
essential requirement for NSF in the organization of myelinated axons
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
N-ethylmaleimide-sensitive factor is required for the synaptic incorporation and removal of 2-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors during cerebellar long-term depression
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
NSF is a critical regulator of leukocyte trafficking in vivo
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
NSF is important in vesicular trafficking, but neural expression of a dominant negative form of NSF2 induces an unexpected overgrowth of the DRosophila larval neuromuscular synapse
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
PEX1 is indispensable for biogenesis and maintenance of the peroxisome
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
Pex1p possesses two distinct oligomeric forms, a homo-oligomer in the cytosol and a hetero-oligomer on peroxisome membranes, possibly playing distinct functions in peroxisome biogenesis
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
the enzyme plays a crucial role in the fusion of vesicles with target membranes. NSF-SNAP complexes may be involved in the disassembly of coiled-coil-mediated interactions underlying not only SNARE complexes but also other forms of stable oligomers, and the C-terminal membrane anchoring of NSF-SNAP targets may be mechanistically involved in the dissociation process
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
the enzyme plays an essential role in intracellular membrane transport events
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
the VCP/Cdc48p homologues C41C4.8 and C06A1.1, AAA chaperones, may play a protective role in polyglutamine aggregation
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
Vps4 disassembles the ESCRT-III protein polymer, thereby changing the morphology of the underlying membrane. The disassembly reaction causes the ESCRT-III subunits to regain the monomeric conformational state, which is the high-energy state that is poised to reassemble again into the ESCRT-III oligomer for subsequent rounds of membrane scission. When assembled, Vps4 progresses through many ATP hydrolysis cycles without dissociation
-
-
?
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
-
the protein p47 forms a tight complex with p97, which is essential for the in vitro Golgi reassmbly
-
additional information
-
Vps4 and its cofactor Vta1p/LIP5 function in membrane remodeling events that accompany cytokinesis, multivesicular body biogenesis, and retrovirus budding
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
NSF is a Ca2+-binding protein, GluR2-NSF interactions are inhibited by the presence of 15 micromol/l Ca2+
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
TAT-NSF700
-
-
-
bafilomycin A
-
-
additional information
-
phosphorylation by Pctaire1 attenuates the hexamerization of NSF
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
p47
-
cofactor to ATPase p97
-
Vta1p
Q97ZJ7
-
-
additional information
-
the protein VCIP135 is essential for p97/p47-mediated membrane fusion
-
additional information
-
Vps4p directly binds Vps20p and Vta1p in vitro
-
additional information
-
SKD1 E Vps proteins SBP1 and mVps2 interact with SKD1
-
additional information
-
activity is dependent upon hexamerisation
-
additional information
-
Ist1, Did2, Vta1 and Vps60 function as a regulatory system that ensures proper recruitment and assembly of ATPase Vps4 on the ESCRT-III protein complex. Directly or indirectly these factors affect ATPase activity of Vps4, suggesting that they also regulate the Vps4 ESCRT-III disassembly activity
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.62
-
MgATP2-
-
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.85
-
ATP
-
higher order oligomer of the enzyme, pH and temperature not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.083
-
-
-
0.72
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.4
-
-
ATPase activity assay
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.4
-
-
ATPase activity assay
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
21
-
-
ATPase activity assay
30
-
-
ATPase activity assay
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
adult, CDC-48.1:GFP fusion construct is expressed from embryos through to adult worms
Manually annotated by BRENDA team
-
superficial layer or deep layer cells of the brain
Manually annotated by BRENDA team
-
mRNA for Hez-NSF is present throughout the embryonic, larval and pupal development
Manually annotated by BRENDA team
-
CDC-48.1:GFP fusion construct is expressed from embryos through to adult worms; CDC-48.2:GFP fusion is mainly expressed in embryos
Manually annotated by BRENDA team
-
from PBD patients
Manually annotated by BRENDA team
-
subesophageal or other
Manually annotated by BRENDA team
-
NFS is primarily expressed in the developing nervous system. At the 14 somite stage, nsf is expressed within cells of the developing trigeminal ganglia, anterior spinal cord, and forebrain. At 20 somites, transcripts are detectable in additional cranial ganglia and throughout the length of the developing spinal cord. By 30 hours post-fertilization, nsf is also visible in regions of the developing midbrain and hindbrain and in the posterior lateral line ganglia. At 48 hours post-fertilization, expression persistes in spinal cord and is present throughout mots of the developing brain and eyes, while at 72 hours post-fertilization, spinal cord expression is less evident. Expression in the posterior lateral line ganglia persists to at least 72 hours post-fertilization
Manually annotated by BRENDA team
-
predominantly expressed in larval and adult nervous system, dNSF-1 is abundant in synaptic vesicle fraction and may have a function in synaptic vesicle release
Manually annotated by BRENDA team
-
N-ethylmaleimide sensitive factor is required for fusion of the Caenorhabditis elegans uterine anchor cell
Manually annotated by BRENDA team
-
MIN-6 beta cell
Manually annotated by BRENDA team
additional information
-
the expression of two p97 homologues CDC-48.1 and CDC-48.2 is differently regulated and independent of each other
Manually annotated by BRENDA team
additional information
-
not present in non-neural tissues
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
localizes mainly in acrosome. ATPase N-ethylmaleimide sensitive factor may be a common feature in the triggering/regulation of membrane merging during mammalian acrosome reaction
-
Manually annotated by BRENDA team
-
Pex1p possesses two distinct oligomeric forms, a homo-oligomer in the cytosol and a hetero-oligomer on peroxisome membranes, possibly playing distinct functions in peroxisome biogenesis
Manually annotated by BRENDA team
-
Pex1p and Pex6p form a stable higher-molecular-mass complex in the cytosol; Pex6p and Pex1p form a stable higher-molecular-mass complex in the cytosol
Manually annotated by BRENDA team
-
multivesicular endosome
Manually annotated by BRENDA team
P46461
NSF requires a peripheral membrane protein to bind Golgi membranes; NSF requires a peripheral membrane protein to bind Golgi membranes
Manually annotated by BRENDA team
-
NSF requires a peripheral membrane protein to bind Golgi membranes
Manually annotated by BRENDA team
-
N-terminal domain of PEX1 is one of the functional modules for membrane binding
Manually annotated by BRENDA team
-
NSF requires a peripheral membrane protein to bind membranes
Manually annotated by BRENDA team
-
the Walker A1 motif of Pex1p is essential for Pex1p-pex6p interaction and Pex1p targeting to peroxisomes
Manually annotated by BRENDA team
-
membrane, Pex1p possesses two distinct oligomeric forms, a homo-oligomer in the cytosol and a hetero-oligomer on peroxisome membranes, possibly playing distinct functions in peroxisome biogenesis; Pex6p is predominantly localized on peroxisomes
Manually annotated by BRENDA team
-
dNSF-1 is absoluteley required for synaptic transmission in the eye
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
28000
-
-
NSF D1 ATPase domain, determined by SDS-PAGE
39000
-
P46467
apo-form, Guinier analysis
48000
-
-
predicted from amino acid sequence
48100
-
-
predicted from amino acid sequence
48500
-
-
predicted molecular mass
50000
-
-
recombinant protein, determined by SDS-PAGE and immunoblot analysis
50000
-
P46467
ADP-bound form, Guinier analysis
60000
-
-
recombinant enzyme, purified from E. coli, gel filtration
85000
-
-
mutant Vps4pdeltaCC/E233Q, gel filtration
95000
-
-
Vps4p, gel filtration
97000
-
-
monomer form of p97, SDS-PAGE
143000
-
-
Pex1p
148000
-
P46467
ATP-bound form, Guinier analysis
245000
-
-
gel filtration
300000
-
-
high-molecular weight form of p97, SDS-PAGE
320000
400000
-
heterodimeric PpPex1p-PpPex6p complex
400000
-
-
mutant Vps4pdeltaCC/E233Q, gel filtration
440000
-
-
mutant Vps4pE233Q, gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
decamer
-
mutants Vps4pE233Q and Vps4pdeltaCC/E233Q, SDS-PAGE
dimer
-
SDS-PAGE
dimer
P52917
Vps4p dimers form two distinct heptameric rings and accommodate AAA cassettes in a head-to-head fashion, X-ray crystallography
dimer
-
the enzyme interconverts between dimers and hexamers in the presence of adenosine nucleotides
dimer
Q97ZJ7
2 * 42248, calculated from sequence, the enzyme rapidly interconverts between multiple oligomeric states, it interconverts between dimers and hexamers in the presence of adenosine nucleotides
dimer
-
2 * 42248, calculated from sequence, the enzyme rapidly interconverts between multiple oligomeric states, it interconverts between dimers and hexamers in the presence of adenosine nucleotides
-
hexamer
-
3D structures of NSF in its functional cycle are reconstructed: NSF in the ATP state, which is capable of binding substrates, the 20S particle, consisting of the NSF hexamer, three alpha-SNAPs and the SNARE complex and NSF in the ADP state. These structures reveal that the two AAA+ domains of NSF, D1 and D2, are in a parallel arrangement in the full-length NSF hexamer, similarly to p97, as shown in its full-length hexamer crystal structures
hexamer
-
the enzyme interconverts between dimers and hexamers in the presence of adenosine nucleotides
hexamer
Q97ZJ7
6 * 42248, calculated from sequence, the enzyme rapidly interconverts between multiple oligomeric states, it interconverts between dimers and hexamers in the presence of adenosine nucleotides
hexamer
-
6 * 42248, calculated from sequence, the enzyme rapidly interconverts between multiple oligomeric states, it interconverts between dimers and hexamers in the presence of adenosine nucleotides
-
homohexamer
P46461
;
homohexamer
-
-
homohexamer
P46462
x-ray crystallography
oligomer
-
-
oligomer
-
Pex1p possesses two distinct oligomeric forms, a homo-oligomer in the cytosol and a hetero-oligomer on peroxisome membranes, possibly playing distinct functions in peroxisome biogenesis. Interaction of Pex1p with Pex6p confers a conformatinal and dissociation of the Pex1p oligomer
monomer
-
1 * 60000, SDS-PAGE
additional information
P46467
addition of ATP but not ADP increases the oligomerized population of SKD1
additional information
-
Vps4 exhibits a dynamic structure that is regulated by the nucleotide-binding state. Oligomerization of the enzyme occurs at protein concentrations above 0.0005 mM in vitro whereas the concentration of Vps4 in vivo is 0.0002 mM. In the cytoplasm the enzyme is dimeric and exhibits no ATPase activity. It is recruited to membrane-associated ESCRT-III where it oligomerizes into the active enzyme
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
phosphoprotein
-
phosphorylated by Pctaire1 on serine 569
phosphoprotein
-
-
phosphoprotein
-
phosphorylated by Pctaire1 on serine 569
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
hanging drop vapour diffusion method, using 5% (w/v) polyethylene glycol 3350 and 100 mM MES (pH 6.0)
P46467
hanging-drop vapour-diffusion method. Preparation of crystals of the N-terminal domain of PEX1. The crystals belong to space group P3(1) or P3(2) with unit-cell parameters a = b = 63.5 A, c = 33.5 A, and contain one protein molecule per crystallographic asymmetric unit. 2.05 A resolution
-
-
protozoa
-
sitting drop vapour diffusion method, at 19°C against a reservoir containing 0.1 M MES buffer (pH 5.9-6.5) and 0.8-1.9 M MgSO4
P52917
Vps4p complexes with and without their MIT N-terminal domains and Vta1p cofactors, hanging drop vapour diffusion method, using
-
sitting-drop vapor diffusion at 20°C
Q97ZJ7
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
recombinant protein is purified using a NTA-Ni2+-agarose cartridge
-
Ni-NTA column chromatography, DEAE Sepharose column chromatography, and Superdex 75 gel filtration
P46467
recombinant NSF is purified by nickel-nitrilotriacetic acid-agarose affinity chromatography
-
HiTrap Ni2+-chelating column chromatography
P46462
protein extracts from cells and brain tissues are prepared
-
Ni-chelating column chromatography and Superdex gel filtration
P52917
Ni2+ Sepharose column chromatography, Q-Sepharose column chromatography, and Superdex 200 gel filtration
-
recombinant protein, expressed in Escherichia coli XL1-blue
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
SKD1 and GFP5 PCR-amplified fragments are cloned in pBSII KS+ and subsequently subcloned into pMZ215S, and in the binary vectors pCambia 1300 and pbinSRNACatN
-
expression in U2OS TRex cells
-
cDNA containing the NSF D1 ATPase domain is inserted into the pET28a vector for expression in Escherichia coli BL21 cells
-
cloning of PEX2, PEX6, PEX12 and PEX1 using yeast genes for homology search
-
isolation of PEX2 and PEX6 cDNA, Pex1p expressed in wild-type chinese hamster ovary cells, CHO-K1
-
His6-NSF for expression in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21(DE3) pLysE cells
P46467
expression in Escherichia coli
-
expression in rat 3Y1B fibroblast cells
-
full-length, partial cDNA fragments and point mutations of mouse NSF are amplified by PCR and subcloned into different expression vectors including pGEX-6P-2, pETH-32, pcDNA3-His6, and pcDNA3-3HA
-
N-terminal domain of PEX1
-
SKD1 cDNA amplified by reverse transcription-PCR
-
expressed in Escherichia coli Rosetta 2 (DE3) cells
P46462
constructing of a genomic library from the haploid yeast strain SD228-7 using the Escherichia coli-yeast shuttle vector pCS19
-
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21(DE3) cells
P52917
expression in Escherichia coli
-
the vectors YCplac111, pLexA, pET11d and pB42AD are used for cloning and expression of wild-type protein, single domains and various mutants
-
VPS24 gene cloned from a yeast genomic library
-
VPS4 gene cloned and overexpressed in Escherichia coli XL1-blue, using a GST fusion vector
-
expression in Escherichia coli
Q97ZJ7
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
E232Q
-
ATPase-deficient mutant
E329Q
-
in contrast to wild-type NFS E329Q mutant is enriched on various structures throughout the cell and is only minimally released by saponin permeabilization
E329Q
-
no ATPase activity
K266A
-
no nucleotide binding
D100A
-
mutant to study the Ca2+ sensitivity of the interaction between NSF and the glutamate receptor subunit 2 of AMPAR
D142A
-
mutant to study the Ca2+ sensitivity of the interaction between NSF and the glutamate receptor subunit 2 of AMPAR
E153A
-
mutant to study the Ca2+ sensitivity of the interaction between NSF and the glutamate receptor subunit 2 of AMPAR
G282E
-
mutant protein has no ATPase activity
E235Q
-
no ATPase activity, mutation leads to perturbation of various membrane transports via endosomes
E305Q
-
mutant assembles into oligomer like wild-type, similar ATPase activity as wild-type
E305Q/E578Q
-
no ATPase activity
E578Q
-
mutant assembles into oligomer like wild-type, severely impaired ATPase activty
S569A
-
mutation of serine 569 to alanine abolishs the phosphorylation of D2 domain by active Pctaire1
K251A
P46462
the p97 mutant carrying mutations in the D2 domain shows little ATPase activity when compared with wild type enzyme; the Walker A mutation to the D1 domain alone shows a moderate decrease in ATPase activity, has reduced affinity for nucleotide in the D1 domain and so contains little prebound ADP
K524A
P46462
the p97 mutant carrying mutations in the D2 domain shows little ATPase activity when compared with wild type enzyme
R359A
P46462
the p97 mutant carrying mutations in the D2 domain shows little ATPase activity when compared with wild type enzyme
R635A
P46462
the p97 mutant carrying mutations in the D2 domain shows little ATPase activity when compared with wild type enzyme
S569A
-
mutation of serine 569 to alanine abolishs the phosphorylation of D2 domain by active Pctaire1
A505L
-
lethal mutation
E233Q
-
no ATP hydrolysis, enhanced binding to Vps20p and Vta1p in vitro
E233Q
-
a mutant protein with very low ATPase activity. In the presence of ADP recombinant mutant enzyme forms dimers. In contrast, the addition of ATP results in the formation of a large complex with at least ten subunits
E329Q
-
hydrolysis mutant
E350Q
-
lethal mutation
E350Q
-
hydrolysis mutant
G89D
-
higher intrinsic ATPase activity than wild-type, no stimulation by Sec17
K179A
-
no ATP binding, enhanced binding to Vps20p and Vta1p in vitro
K266A
-
ATP-binding mutant
Q216A
P52917
monomeric mutant form
K549A
-
decrease in NSF vesicular transport activity
additional information
-
NSF DELTA-N mutant fails to bind to SNAP-SNARE complex
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
medicine
-
dysfunction of p97 has serious pathological consequences and has been implicated in a variety of cancers and neurodegenerative deseases
medicine
-
diagnosis, HsPex6p mutations are one of the causes of Zellweger syndrome
medicine
-
HsPEX1 is the causative gene for peroxisome-deficiency autosomal recessive disorders like cerebro-hepato-renal Zellweger syndrome, neonatal adrenoleukodystrophy and infantile refsum disease
medicine
-
dysfunction of p97 has serious pathological consequences and has been implicated in a variety of cancers and neurodegenerative deseases