Cloned (Comment) | Organism |
---|---|
expression of a His-tagged protein in Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,2,3,4-tetra(adenosine-5'-O-phospho) erythritol | - |
Escherichia coli | |
1,2,3,4-tetra(adenosine-5'-O-phosphorothio) erythritol | competitive with respect to Ap4A | Escherichia coli | |
1,2,3,4-tetra(adenosine-5'-O-phosphorothio) erythritol | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
1,2,3-tri(adenosine-5'-O-phospho) glycerol | - |
Escherichia coli | |
1,3-di(adenosine-5'-O-phospho) glycerol | - |
Escherichia coli | |
1,3-di(adenosine-5'-O-phospho)-2-phosphoglycerol | - |
Escherichia coli | |
1,3-di(adenosine-5'-O-phosphorothio) glycerol | - |
Escherichia coli | |
1,3-di(adenosine-5'-O-phosphorothio)-2-phosphorothioglycerol | - |
Escherichia coli | |
1,3-di(adenosine-5'-O-phosphorothio)-2-phosphorothioglycerol | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
1,4-di(adenosine-5'-O-phosphorothio) butanediol | - |
Escherichia coli | |
1,4-di(adenosine-5'-O-phosphorothio) erythritol | - |
Escherichia coli | |
1,5-di(adenosine-5'-O-phosphorothio) pentanediol | - |
Escherichia coli | |
di(adenosine-5'-O-phospho)-di(phospho) pentaerythritol | - |
Escherichia coli | |
di(adenosine-5'-O-phosphorothio)-di(phosphorothio) pentaerythritol | competitive with respect to Ap4A | Escherichia coli | |
di(adenosine-5'-O-phosphorothio)-di(phosphorothio) pentaerythritol | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
tetra(adenosine-5'-O-phospho) pentaerythritol | - |
Escherichia coli | |
tetra(adenosine-5'-O-phosphorothio) pentaerythritol | - |
Escherichia coli | |
tetra(adenosine-5'-O-phosphorothio) pentaerythritol | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
tri(adenosine-5'-O-phospho)-phospho pentaerythritol | - |
Escherichia coli | |
tri(adenosine-5'-O-phosphorothio) glycerol | - |
Escherichia coli | |
tri(adenosine-5'-O-phosphorothio)-phosphorothio pentaerythritol | - |
Escherichia coli | |
tri(adenosine-5'-O-phosphorothio)-phosphorothio pentaerythritol | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | pH 7.6, 30°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.025 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | pH 7.6, 30°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K12 strain | - |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
LT2 strain | - |
Purification (Comment) | Organism |
---|---|
using Ni-affinity column chromatography to isolate a His-tagged recombinant enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 2 ADP | - |
? | |
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O | hydrolysis of Ap4A | Escherichia coli | 2 ADP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
(symmetrical) Ap4A hydrolase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
(symmetrical) Ap4A hydrolase | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00002 | - |
di(adenosine-5'-O-phosphorothio)-di(phosphorothio) pentaerythritol | pH 7.6, 30°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.00004 | - |
di(adenosine-5'-O-phosphorothio)-di(phosphorothio) pentaerythritol | pH 7.6, 30°C | Escherichia coli | |
0.00008 | - |
tri(adenosine-5'-O-phosphorothio)-phosphorothio pentaerythritol | pH 7.6, 30°C | Escherichia coli | |
0.001 | - |
di(adenosine-5'-O-phospho)-di(phospho) pentaerythritol | pH 7.6, 30°C | Escherichia coli | |
0.0013 | - |
1,2,3,4-tetra(adenosine-5'-O-phosphorothio) erythritol | pH 7.6, 30°C | Escherichia coli | |
0.0014 | - |
1,3-di(adenosine-5'-O-phosphorothio)-2-phosphorothioglycerol | pH 7.6, 30°C | Escherichia coli | |
0.0014 | - |
tetra(adenosine-5'-O-phosphorothio) pentaerythritol | pH 7.6, 30°C | Escherichia coli | |
0.0017 | - |
tri(adenosine-5'-O-phosphorothio)-phosphorothio pentaerythritol | pH 7.6, 30°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.0018 | - |
tetra(adenosine-5'-O-phosphorothio) pentaerythritol | pH 7.6, 30°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.0029 | - |
1,2,3,4-tetra(adenosine-5'-O-phosphorothio) erythritol | pH 7.6, 30°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.003 | - |
tri(adenosine-5'-O-phospho)-phospho pentaerythritol | pH 7.6, 30°C | Escherichia coli | |
0.0039 | - |
1,3-di(adenosine-5'-O-phosphorothio)-2-phosphorothioglycerol | pH 7.6, 30°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.005 | - |
1,3-di(adenosine-5'-O-phospho)-2-phosphoglycerol | pH 7.6, 30°C | Escherichia coli | |
0.006 | - |
1,2,3,4-tetra(adenosine-5'-O-phospho) erythritol | pH 7.6, 30°C | Escherichia coli | |
0.007 | - |
tri(adenosine-5'-O-phosphorothio) glycerol | pH 7.6, 30°C | Escherichia coli | |
0.028 | - |
tetra(adenosine-5'-O-phospho) pentaerythritol | pH 7.6, 30°C | Escherichia coli | |
0.078 | - |
1,4-di(adenosine-5'-O-phosphorothio) erythritol | pH 7.6, 30°C | Escherichia coli | |
0.089 | - |
1,4-di(adenosine-5'-O-phosphorothio) butanediol | pH 7.6, 30°C | Escherichia coli | |
0.109 | - |
1,5-di(adenosine-5'-O-phosphorothio) pentanediol | pH 7.6, 30°C | Escherichia coli | |
0.157 | - |
1,3-di(adenosine-5'-O-phosphorothio) glycerol | pH 7.6, 30°C | Escherichia coli | |
0.215 | - |
1,3-di(adenosine-5'-O-phospho) glycerol | pH 7.6, 30°C | Escherichia coli | |
4.08 | - |
1,2,3-tri(adenosine-5'-O-phospho) glycerol | pH 7.6, 30°C | Escherichia coli |