Information on EC 3.6.1.41 - bis(5'-nucleosyl)-tetraphosphatase (symmetrical)

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
3.6.1.41
-
RECOMMENDED NAME
GeneOntology No.
bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O = 2 ADP
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of diphosphate bonds
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Purine metabolism
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-
purine metabolism
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-
SYSTEMATIC NAME
IUBMB Comments
P1,P4-bis(5'-nucleosyl)-tetraphosphate nucleosidebisphosphohydrolase
Also acts on bis(5'-guanosyl) tetraphosphate and bis(5'-adenosyl) pentaphosphate and, more slowly, on some other polyphosphates, forming a nucleoside bisphosphate as one product in all cases [cf. EC 3.6.1.17 bis(5'-nucleosyl)-tetraphosphatase (asymmetrical)].
CAS REGISTRY NUMBER
COMMENTARY hide
85638-48-8
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-adenylated bis(5'-adenosyl) tetraphosphate + H2O
3'-adenylated ADP + ADP
show the reaction diagram
-
hydrolysis of 3'-adenylated Ap3A
-
-
?
3'-adenylated bis(5'-guanosyl) tetraphosphate + H2O
3'-adenylated GDP + GDP
show the reaction diagram
-
hydrolysis of 3'-adenylated Gp3G
-
-
?
A5'-P(S)PPP-5'A + H2O
ADP + ADPalphaS
show the reaction diagram
-
-
-
?
adenosine 5'-P(1)-tetraphosphate-P(4)-5'''-cytidine + H2O
ADP + CDP
show the reaction diagram
-
-
-
?
adenosine 5'-P(1)-triphosphate-P(3)-5''-cytidine + H2O
ADP + CMP + CDP + AMP
show the reaction diagram
-
-
-
?
adenosine 5'-P(1)-triphosphate-P(3)-5''-guanosine + H2O
ADP + GMP
show the reaction diagram
-
-
-
?
Ap3A + H2O
ADP + AMP
show the reaction diagram
Ap4 + H2O
?
show the reaction diagram
-
-
-
-
?
Ap4G + H2O
ADP + GDP
show the reaction diagram
-
-
-
?
ApCF2ppCF2pA + H2O
?
show the reaction diagram
-
at 0.2% the rate of P1,P4-bis(5'-adenosyl) tetraphosphate
-
-
?
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
ADP + ATP
show the reaction diagram
diadenosine tetraphosphate + H2O
2 adenosine diphosphate
show the reaction diagram
Gp3C + H2O
CDP + GMP
show the reaction diagram
-
-
-
?
Gp3G + H2O
guanosine 3'-diphosphate + guanosine 5'-phosphate
show the reaction diagram
Gp5G + H2O
GDP + GTP
show the reaction diagram
-
-
-
?
Gp6G + H2O
GDP + guanosine 5'-tetraphosphate
show the reaction diagram
-
-
-
?
P1,P4-bis(5'-(2'-deoxy)adenosyl)tetraphosphate + H2O
2 dADP
show the reaction diagram
-
-
-
?
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
2 ADP
show the reaction diagram
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
ADP
show the reaction diagram
P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O
GTP
show the reaction diagram
P1,P4-bis(adenosyl)tetraphosphate + H2O
2 ADP
show the reaction diagram
P1,P5-bis(adenosyl)pentaphosphate + H2O
ADP + ATP
show the reaction diagram
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O
ADP + ?
show the reaction diagram
-
hydrolysis of Ap6A
-
-
?
P1,P6-di(adenosine-5')hexaphosphate + H2O
ADP + adenosine 5'-tetraphosphate
show the reaction diagram
p4G + H2O
GDP + diphosphate
show the reaction diagram
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-
-
?
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cd2+
-
0.002-0.005 mM, 3.5fold stimulation
Mg2+
-
weak stimulation in absence of other divalent metal ions, high synergistic stimulation in combination with Co2+ at its suboptimal concentrations
Ni2+
-
0.004-0.008 mM, 3.5fold stimulation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
-
1,2,3,4-tetra(adenosine-5'-O-phospho) erythritol
-
-
1,2,3,4-tetra(adenosine-5'-O-phosphorothio) erythritol
1,2,3-tri(adenosine-5'-O-phospho) glycerol
-
-
1,3-di(adenosine-5'-O-phospho) glycerol
-
-
1,3-di(adenosine-5'-O-phospho)-2-phosphoglycerol
-
-
1,3-di(adenosine-5'-O-phosphorothio) glycerol
-
-
1,3-di(adenosine-5'-O-phosphorothio)-2-phosphorothioglycerol
1,4-di(adenosine-5'-O-phosphorothio) butanediol
-
-
1,4-di(adenosine-5'-O-phosphorothio) erythritol
-
-
1,5-di(adenosine-5'-O-phosphorothio) pentanediol
-
-
A-5'-pCH2OpOCH2p-5'-A
-
no activity as inhibitor of Escherichia coli symmetrical Ap4A hydrolase
A-5'-pCH2OpOpOCH2p-5'-A
-
no activity as inhibitor of Escherichia coli symmetrical Ap4A hydrolase
A-5'-pOCH2pCH2Op-5'-A
-
no activity as inhibitor of Escherichia coli symmetrical Ap4A hydrolase
A-5'-pOCH2pOpCH2Op-5'-A
-
no activity as inhibitor of Escherichia coli symmetrical Ap4A hydrolase
A-5'-pOpCH2OpOp-5'-A
-
no activity as inhibitor of Escherichia coli symmetrical Ap4A hydrolase
A-5'-pOpOCH2pCH2OpOp-5'-A
-
no activity as inhibitor of Escherichia coli symmetrical Ap4A hydrolase
adenosine 5'-tetraphosphate
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Ap(CH2)pp(CH2)pA
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App(CH2)ppA
-
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App(CHBr)ppA
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Appp(CH2)pA
-
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di(adenosine-5'-O-phospho)-di(phospho) pentaerythritol
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di(adenosine-5'-O-phosphorothio)-di(phosphorothio) pentaerythritol
glycerol phosphate
20 mM, 33.4% residual activity; 20 mM, 46.1% residual activity
guanosine 5'-tetraphosphate
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-
iodoacetic acid
10 mM, 58.8% residual activity
N-ethylmaleimide
1 mM, 62.6% residual activity; 1 mM, 73.8% residual activity
sodium orthovanadate
1 mM, 6.7% residual activity
tetra(adenosine-5'-O-phospho) pentaerythritol
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-
tetra(adenosine-5'-O-phosphorothio) pentaerythritol
tri(adenosine-5'-O-phospho)-phospho pentaerythritol
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tri(adenosine-5'-O-phosphorothio) glycerol
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tri(adenosine-5'-O-phosphorothio)-phosphorothio pentaerythritol
trisodium phosphate
20 mM, 24.8% residual activity; 20 mM, 6.2% residual activity
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.005 - 0.037
P1,P4-bis(5'-adenosyl) tetraphosphate
0.0026
P1,P4-bis(5'-adenosyl)tetraphosphate
-
and 0.037, biphasic kinetics
0.14 - 0.86
P1,P4-bis(adenosyl)tetraphosphate
-
0.19 - 0.49
P1,P5-bis(adenosyl)pentaphosphate
-
0.014
P1,P6-bis(5'-adenosyl)hexaphosphate
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reaction medium contains 0.1 mM CoCl2
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
250
P1,P4-bis(5'-adenosyl) tetraphosphate
Escherichia coli
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-
0.055 - 7.12
P1,P4-bis(adenosyl)tetraphosphate
-
0.035 - 24
P1,P5-bis(adenosyl)pentaphosphate
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.4 - 8.28
P1,P4-bis(adenosyl)tetraphosphate
206955
0.18 - 48.57
P1,P5-bis(adenosyl)pentaphosphate
206956
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.006
1,2,3,4-tetra(adenosine-5'-O-phospho) erythritol
-
pH 7.6, 30C
0.0013 - 0.0029
1,2,3,4-tetra(adenosine-5'-O-phosphorothio) erythritol
4.08
1,2,3-tri(adenosine-5'-O-phospho) glycerol
-
pH 7.6, 30C
0.215
1,3-di(adenosine-5'-O-phospho) glycerol
-
pH 7.6, 30C
0.005
1,3-di(adenosine-5'-O-phospho)-2-phosphoglycerol
-
pH 7.6, 30C
0.157
1,3-di(adenosine-5'-O-phosphorothio) glycerol
-
pH 7.6, 30C
0.0014 - 0.0039
1,3-di(adenosine-5'-O-phosphorothio)-2-phosphorothioglycerol
0.089
1,4-di(adenosine-5'-O-phosphorothio) butanediol
-
pH 7.6, 30C
0.078
1,4-di(adenosine-5'-O-phosphorothio) erythritol
-
pH 7.6, 30C
0.109
1,5-di(adenosine-5'-O-phosphorothio) pentanediol
-
pH 7.6, 30C
0.001
di(adenosine-5'-O-phospho)-di(phospho) pentaerythritol
-
pH 7.6, 30C
0.00002 - 0.00004
di(adenosine-5'-O-phosphorothio)-di(phosphorothio) pentaerythritol
0.028
tetra(adenosine-5'-O-phospho) pentaerythritol
-
pH 7.6, 30C
0.0014 - 0.0018
tetra(adenosine-5'-O-phosphorothio) pentaerythritol
0.003
tri(adenosine-5'-O-phospho)-phospho pentaerythritol
-
pH 7.6, 30C
0.007
tri(adenosine-5'-O-phosphorothio) glycerol
-
pH 7.6, 30C
0.00008 - 0.0017
tri(adenosine-5'-O-phosphorothio)-phosphorothio pentaerythritol
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
26000
-
Physarum polycephalum
27000
-
gel filtration
32000
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SDS-PAGE
33000
-
gel filtration
34000
-
1 * 34000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drop vapour diffusion method using PEG 550 MME as precipitant
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-chelating Sepharose column chromatography, Resource-Q anion exchange chromatography and HiLoad 16/60 Superdex G75 column chromatography
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symmetrical Ap4A hydrolase is partially purified
-
using Ni-affinity column chromatography to isolate a His-tagged recombinant enzyme
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli strain BL21(D3)
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expression in Escherichia coli of a His-tagged recombinant protein
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expression of a His-tagged protein in Escherichia coli
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
generation of null mutants by replacement of the gene encoding the enzyme with antibiotic resistance cassettes, the resulting STYA201 strain shows reduced ability to invade cultured mammalian cells
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