Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Escherichia coli | |
Cu2+ | - |
Acidaminococcus fermentans | |
Cu2+ | no effect below 0.03 mM, inhibition above | Escherichia coli | |
Cu2+ | - |
Pyrodictium occultum | |
Fe2+ | no effect below 0.03 mM, inhibition above | Acidaminococcus fermentans | |
Fe2+ | no effect below 0.03 mM, inhibition above | Escherichia coli | |
Fe2+ | no effect below 0.03 mM, inhibition above | Pyrodictium occultum | |
Zn2+ | no effect below 0.03 mM, inhibition above | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | weak stimulation in absence of other divalent metal ions, high synergistic stimulation in combination with Co2+ at its suboptimal concentration | Escherichia coli | |
Cd2+ | 0.002-0.005 mM, 3.5fold stimulation | Escherichia coli | |
Co2+ | 0.1-0.5 mM, 100fold increase of activity | Escherichia coli | |
Mg2+ | weak stimulation in absence of other divalent metal ions, high synergistic stimulation in combination with Co2+ at its suboptimal concentrations | Escherichia coli | |
Mn2+ | 0.2-0.5 mM, 8fold stimulation | Escherichia coli | |
Ni2+ | 0.004-0.008 mM, 3.5fold stimulation | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidaminococcus fermentans | - |
- |
- |
Escherichia coli | - |
K12 | - |
Pyrodictium occultum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.062 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ap3A + H2O | - |
Escherichia coli | ADP + AMP | - |
? | |
Ap4 + H2O | - |
Escherichia coli | ? | - |
? | |
diadenosine 5',5''-P1,P5-pentaphosphate + H2O | - |
Escherichia coli | ADP + ATP | - |
? | |
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O | - |
Escherichia coli | ADP | - |
? | |
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O | - |
Acidaminococcus fermentans | ADP | - |
? | |
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O | - |
Pyrodictium occultum | ADP | - |
? | |
P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O | - |
Escherichia coli | GTP | - |
? | |
P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O | - |
Acidaminococcus fermentans | GTP | - |
? | |
P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O | - |
Pyrodictium occultum | GTP | - |
? | |
P1,P6-di(adenosine-5')hexaphosphate + H2O | - |
Escherichia coli | ADP + adenosine 5'-tetraphosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
- |
Escherichia coli |