Protein Variants | Comment | Organism |
---|---|---|
H116C | site-directed mutagenesis. Mutant binds 0.33 equiv of Zn2+ | Stenotrophomonas maltophilia |
H118C | site-directed mutagenesis | Stenotrophomonas maltophilia |
H121C | site-directed mutagenesis. Mutant binds 0.11 equiv of Zn2+ | Stenotrophomonas maltophilia |
H196C | site-directed mutagenesis | Stenotrophomonas maltophilia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0023 | - |
nitrocefin | lactamase ZnCo-L1, 1.0 Zn(2+), 1.0 Co(2+) | Stenotrophomonas maltophilia | |
0.0036 | - |
nitrocefin | lactamase ZnFe-L1, 1.0 Zn(2+), 1.0 Fe(3+) | Stenotrophomonas maltophilia | |
0.004 | - |
nitrocefin | lactamase ZnFe-L1, 1.2 Zn(2+), 0.9 Fe(3+) | Stenotrophomonas maltophilia | |
0.004 | - |
nitrocefin | wild-typewith 1.90 equiv of Zn(2+) | Stenotrophomonas maltophilia | |
0.0043 | - |
nitrocefin | lactamase 1Co-L1, 0.9 Co(2+), 0.10 Zn(2+) | Stenotrophomonas maltophilia | |
0.0055 | - |
nitrocefin | lactamase 1Zn-L1, 1.0 Zn(2+) | Stenotrophomonas maltophilia | |
0.0059 | - |
nitrocefin | lactamase ZnZn-L1, 2.0 Zn(2+) added | Stenotrophomonas maltophilia | |
0.018 | - |
nitrocefin | H116C, not applicable Zn(2+) content | Stenotrophomonas maltophilia | |
0.02 | - |
nitrocefin | H116C with 0.85 equiv of Zn(2+) | Stenotrophomonas maltophilia | |
0.02 | - |
nitrocefin | lactamase CoCo-L1, 1.80 Co(2+) | Stenotrophomonas maltophilia | |
0.053 | - |
nitrocefin | lactamase 1Fe-L1, 0.9 Co(2+), 0.20 Zn(2+) | Stenotrophomonas maltophilia | |
0.072 | - |
nitrocefin | H121C with 0.98 equiv of Zn(2+) | Stenotrophomonas maltophilia | |
0.099 | - |
nitrocefin | H121C, not applicable Zn(2+) content | Stenotrophomonas maltophilia |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | mononuclear Co(2+)- and Fe(3+)-containing lactamase L1 are essentially inactive | Stenotrophomonas maltophilia | |
Fe3+ | mononuclear Co(2+)- and Fe(3+)-containing lactamase L1 are essentially inactive | Stenotrophomonas maltophilia | |
additional information | mononuclear metal ion containing and heterobimetallic analogues of the enzyme are generated and characterized using kinetic and spectroscopic studies. Heterobimetallic analogues (ZnCoL1, ZnFeL1, FeFeL1, and CoCoL1) analogues of L1 are generated, and stopped flow kinetic studies revealed that these enzymes rapidly hydrolyze nitrocefin and that there are large amounts of the reaction intermediate formed during the reaction | Stenotrophomonas maltophilia | |
Zn2+ | metalloenzyme | Stenotrophomonas maltophilia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Stenotrophomonas maltophilia | P52700 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrocefin + H2O | - |
Stenotrophomonas maltophilia | (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
metallo-beta-lactamase L1 | - |
Stenotrophomonas maltophilia |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.6 | - |
nitrocefin | lactamase 1Fe-L1, 0.9 Co(2+), 0.20 Zn(2+) | Stenotrophomonas maltophilia | |
11 | - |
nitrocefin | lactamase 1Co-L1, 0.9 Co(2+), 0.10 Zn(2+) | Stenotrophomonas maltophilia | |
20 | - |
nitrocefin | lactamase ZnFe-L1, 1.0 Zn(2+), 1.0 Fe(3+) | Stenotrophomonas maltophilia | |
24 | - |
nitrocefin | lactamase ZnFe-L1, 1.2 Zn(2+), 0.9 Fe(3+) | Stenotrophomonas maltophilia | |
26 | - |
nitrocefin | lactamase ZnCo-L1, 1.0 Zn(2+), 1.0 Co(2+) | Stenotrophomonas maltophilia | |
30 | - |
nitrocefin | lactamase 1Zn-L1, 1.0 Zn(2+) | Stenotrophomonas maltophilia | |
39 | - |
nitrocefin | lactamase ZnZn-L1, 2.0 Zn(2+) added | Stenotrophomonas maltophilia | |
63 | - |
nitrocefin | lactamase CoCo-L1, 1.80 Co(2+) | Stenotrophomonas maltophilia |