Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli TBK-1 cells | Kluyvera ascorbata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
cefoperazone | in 0.01 M phosphate buffer (pH 7.0), at 25°C | Kluyvera ascorbata | |
0.0034 | - |
Imipenem | in 0.01 M phosphate buffer (pH 7.0), at 25°C | Kluyvera ascorbata | |
0.01 | - |
cefoxitin | in 0.01 M phosphate buffer (pH 7.0), at 25°C | Kluyvera ascorbata | |
0.011 | - |
piperacillin | in 0.01 M phosphate buffer (pH 7.0), at 25°C | Kluyvera ascorbata | |
0.015 | - |
ampicillin | in 0.01 M phosphate buffer (pH 7.0), at 25°C | Kluyvera ascorbata | |
0.016 | - |
benzylpenicillin | in 0.01 M phosphate buffer (pH 7.0), at 25°C | Kluyvera ascorbata | |
0.017 | - |
carbenicillin | in 0.01 M phosphate buffer (pH 7.0), at 25°C | Kluyvera ascorbata | |
0.019 | - |
cefuroxime | in 0.01 M phosphate buffer (pH 7.0), at 25°C | Kluyvera ascorbata | |
0.043 | - |
cefotaxime | in 0.01 M phosphate buffer (pH 7.0), at 25°C | Kluyvera ascorbata | |
0.047 | - |
nitrocefin | in 0.01 M phosphate buffer (pH 7.0), at 25°C | Kluyvera ascorbata | |
0.049 | - |
cephalothin | in 0.01 M phosphate buffer (pH 7.0), at 25°C | Kluyvera ascorbata | |
0.39 | - |
aztreonam | in 0.01 M phosphate buffer (pH 7.0), at 25°C | Kluyvera ascorbata | |
1 | - |
ceftazidime | Km above 1.0 mM, in 0.01 M phosphate buffer (pH 7.0), at 25°C | Kluyvera ascorbata | |
1 | - |
cefepime | Km above 1.0 mM, in 0.01 M phosphate buffer (pH 7.0), at 25°C | Kluyvera ascorbata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28120 | - |
theoretical molecular weight | Kluyvera ascorbata |
28200 | - |
SDS-PAGE | Kluyvera ascorbata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kluyvera ascorbata | - |
- |
- |
Kluyvera ascorbata 68 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sephadex column chromatography and Sephadex G-100 gel filtration | Kluyvera ascorbata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ampicillin + H2O | - |
Kluyvera ascorbata | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
ampicillin + H2O | - |
Kluyvera ascorbata 68 | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
aztreonam + H2O | - |
Kluyvera ascorbata | [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate | - |
? | |
aztreonam + H2O | - |
Kluyvera ascorbata 68 | [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate | - |
? | |
benzylpenicillin + H2O | - |
Kluyvera ascorbata | (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
benzylpenicillin + H2O | - |
Kluyvera ascorbata 68 | (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
carbenicillin + H2O | - |
Kluyvera ascorbata | (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
cefepime + H2O | - |
Kluyvera ascorbata | (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
cefoperazone + H2O | - |
Kluyvera ascorbata | (2R)-2-[(R)-carboxy{[(2S)-2-[(4-ethyl-2,3-dioxopiperazine-1-carbonyl)amino]-2-(4-hydroxyphenyl)acetyl]amino}methyl]-5-{[(1-methyl-1H-tetrazol-5-yl)sulfanyl]methyl}-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cefotaxime + H2O | - |
Kluyvera ascorbata | (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cefoxitin + H2O | - |
Kluyvera ascorbata | (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
ceftazidime + H2O | - |
Kluyvera ascorbata | (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
cefuroxime + H2O | - |
Kluyvera ascorbata | (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cephalothin + H2O | - |
Kluyvera ascorbata | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cephalothin + H2O | - |
Kluyvera ascorbata 68 | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
imipenem + H2O | - |
Kluyvera ascorbata | (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
additional information | dicloxacillin, cefoxitin and imipenem behave as poor substrates | Kluyvera ascorbata | ? | - |
? | |
additional information | dicloxacillin, cefoxitin and imipenem behave as poor substrates | Kluyvera ascorbata 68 | ? | - |
? | |
nitrocefin + H2O | - |
Kluyvera ascorbata | (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
piperacillin + H2O | - |
Kluyvera ascorbata | (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
KLUA-9 | - |
Kluyvera ascorbata |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00024 | - |
Imipenem | in 0.01 M phosphate buffer (pH 7.0), at 25°C | Kluyvera ascorbata | |
0.00043 | - |
cefoxitin | in 0.01 M phosphate buffer (pH 7.0), at 25°C | Kluyvera ascorbata | |
0.22 | - |
cefoperazone | in 0.01 M phosphate buffer (pH 7.0), at 25°C | Kluyvera ascorbata | |
0.24 | - |
aztreonam | in 0.01 M phosphate buffer (pH 7.0), at 25°C | Kluyvera ascorbata | |
1 | - |
carbenicillin | in 0.01 M phosphate buffer (pH 7.0), at 25°C | Kluyvera ascorbata | |
2.5 | - |
cefuroxime | in 0.01 M phosphate buffer (pH 7.0), at 25°C | Kluyvera ascorbata | |
3.3 | - |
cefotaxime | in 0.01 M phosphate buffer (pH 7.0), at 25°C | Kluyvera ascorbata | |
5 | - |
piperacillin | in 0.01 M phosphate buffer (pH 7.0), at 25°C | Kluyvera ascorbata | |
7 | - |
ampicillin | in 0.01 M phosphate buffer (pH 7.0), at 25°C | Kluyvera ascorbata | |
10 | - |
benzylpenicillin | in 0.01 M phosphate buffer (pH 7.0), at 25°C | Kluyvera ascorbata | |
29 | - |
nitrocefin | in 0.01 M phosphate buffer (pH 7.0), at 25°C | Kluyvera ascorbata | |
56 | - |
cephalothin | in 0.01 M phosphate buffer (pH 7.0), at 25°C | Kluyvera ascorbata |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Kluyvera ascorbata | isoelectric focusing | - |
7.7 |