Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Klebsiella pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ceftazidime | acts as a very weak simple noncompetitive inhibitor | Klebsiella pneumoniae | |
Imipenem | - |
Klebsiella pneumoniae | |
KCl | concentrations of KCl higher than 20 mM weaken slightly the hydrolytic ability of VIM-12 | Klebsiella pneumoniae | |
meropenem | - |
Klebsiella pneumoniae | |
additional information | no inhibitory effect by aztreonam | Klebsiella pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.094 | - |
Imipenem | - |
Klebsiella pneumoniae | |
0.28 | - |
penicillin G | - |
Klebsiella pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | dependent | Klebsiella pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
- |
- |
Klebsiella pneumoniae 2873 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
imipenem + H2O | moderate rate of hydrolysis | Klebsiella pneumoniae | (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
imipenem + H2O | moderate rate of hydrolysis | Klebsiella pneumoniae 2873 | (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
additional information | no hydrolysis of aztreonam, meropenem, ceftazidime, cefoxitin, and cefotaxime | Klebsiella pneumoniae | ? | - |
? | |
additional information | no hydrolysis of aztreonam, meropenem, ceftazidime, cefoxitin, and cefotaxime | Klebsiella pneumoniae 2873 | ? | - |
? | |
penicillin G + H2O | efficient hydrolysis | Klebsiella pneumoniae | (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
penicillin G + H2O | efficient hydrolysis | Klebsiella pneumoniae 2873 | (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MBL | - |
Klebsiella pneumoniae |
metallo-beta-lactamase | - |
Klebsiella pneumoniae |
VIM-12 | a hybrid VIM-1/VIM-2 metallo-beta-lactamase | Klebsiella pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Klebsiella pneumoniae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
Imipenem | at 40?C in 50 mM HEPES buffer (pH 6.8) | Klebsiella pneumoniae | |
34 | - |
penicillin G | at 40?C in 50 mM HEPES buffer (pH 6.8) | Klebsiella pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | - |
- |
Klebsiella pneumoniae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0042 | - |
meropenem | at 40?C in 50 mM HEPES buffer (pH 6.8) | Klebsiella pneumoniae | |
0.0806 | - |
Imipenem | at 40?C in 50 mM HEPES buffer (pH 6.8) | Klebsiella pneumoniae |