Literature summary for 3.5.2.2 extracted from

Aranaz, I.; Acosta, N.; Heras, A.
Encapsulation of an Agrobacterium radiobacter extract containing D-hydantoinase and D-carbamoylase activities into alginate-chitosan polyelectrolyte complexes. Preparation of the biocatalyst (2009), J. Mol. Catal. B, 58, 54-64.

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Application

Application	Comment	Organism
synthesis	encapsulation of a crude cell extract from Agrobacterium radiobacter containing D-hydantoinase and D-carbamoylase activities into alginate-chitosan polyelectrolyte complexes with negligible leakage from the formed capsules. The most suitable biocatalysts are prepared using a chitosan with a medium molecular weight of 600 kDa and a degree of deacetylation of 0.9. For all of the preparation conditions under study, an encapsulation yield of around 60% was achieved and the enzymatic activity yields ranged from 30 to 80% for D-hydantoinase activity and from 40 to 128% for D-carbamoylase activity relative to the activities of the soluble extract. All of the biocatalysts are able to hydrolyze LD-hydroxyphenylhydantoin into p-hydroxyphenylglycine with yields ranging from 30 to 80%	Agrobacterium tumefaciens

Organism

Organism	UniProt	Comment	Textmining
Agrobacterium tumefaciens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DL-5-phenylhydantoin + H2O	-	Agrobacterium tumefaciens	N-carbamoyl-D-phenylglycine	-	?

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Release 2023.1 (January 2023)