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Literature summary for 3.5.2.2 extracted from
- The immobilization of D-hydantoinase and characterization under classic condition and microwave irradiation (2006), J. Mol. Catal. B, 43, 74-79.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| reactions catalyzed by both free and immobilized D-hydantoinase are accelerated under microwave irradiation | Burkholderia cepacia |
| the half-time of the immobilized D-hydantoinase (onto polystyrene anion exchange resin via glutaraldehyde) is 11 days | Burkholderia cepacia |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 14.98 | - |
D,L-benzylhydantoin | free enzyme, at pH 8.5 and 45°C | Burkholderia cepacia |  |
| 27.03 | - |
D,L-benzylhydantoin | immobilized enzyme, at pH 8.5 and 45°C | Burkholderia cepacia | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia cepacia | - |
- |
- |
| Burkholderia cepacia JS-02 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Phenyl Sepharose Fast Flow column chromatography | Burkholderia cepacia |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, onto polystyrene anion exchange resin via glutaraldehyde-immobilized D-hydantoinase, 100 days, nearly 90% of activity is reserved | Burkholderia cepacia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D,L-benzylhydantoin + H2O | - |
Burkholderia cepacia | ? | - |
? | |
| D,L-benzylhydantoin + H2O | - |
Burkholderia cepacia JS-02 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-hydantoinase | - |
Burkholderia cepacia |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 38 | - |
free enzyme | Burkholderia cepacia |
| 45 | - |
immobilized enzyme | Burkholderia cepacia |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 25 | the relative activity of immobilized D-hydantoinase is dramatically enhanced when the temperature increased from 5 to 25°C | Burkholderia cepacia |
| 60 | - |
at 60°C, the immobilized D-hydantoinase remains 90% activity at least, but the immobilized enzyme form decreases sharply to 65% of the activity | Burkholderia cepacia |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
free enzyme | Burkholderia cepacia |
| 9 | - |
immobilized enzyme | Burkholderia cepacia |
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Release 2023.1 (January 2023)
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