Protein Variants | Comment | Organism |
---|---|---|
F159A | turnover number for hydantoin is 10% of that of the wild-type enzyme, Km-value for hydantoin is 4.6fold higher than that of the wild-type enzyme, turnover-number for DL-hydroxyphenylhydantoin is 6.1fold higher than that of the wild-type enzyme, Km-value for DL-hydroxyphenylhydantoin is 1.4fold higher than that of the wild-type enzyme. Specificity of the mutant enzyme towards aromatic substrate hydroxyphenylhydantoin is enhanced by 200fold compared with that of the wild-type enzyme | Geobacillus stearothermophilus |
F159I | turnover number for hydantoin is 61% of that of the wild-type enzyme, Km-value for hydantoin is 1.9fold higher than that of the wild-type enzyme, turnover-number for DL-hydroxyphenylhydantoin is 2.6fold higher than that of the wild-type enzyme, Km-value for DL-hydroxyphenylhydantoin is 1.5fold lower than that of the wild-type enzyme | Geobacillus stearothermophilus |
F159L | turnover number for hydantoin 64% of the turnover number of the wild-type enzyme, Km-value for hydantoin is 90% of the value of the wild-type enzyme, turnover-number for DL-hydroxyphenylhydantoin is 61% of that of the wild-type enzyme, Km-value for DL-hydroxyphenylhydantoin is 2.5fold lower than that of the wild-type enzyme | Geobacillus stearothermophilus |
F159S | turnover number for hydantoin is 4.7% of that of the wild-type enzyme, Km-value for hydantoin is 4fold higher than that of the wild-type enzyme, turnover-number for DL-hydroxyphenylhydantoin is 15.5fold higher than that of the wild-type enzyme, Km-value for DL-hydroxyphenylhydantoin is 5fold higher than that of the wild-type enzyme | Geobacillus stearothermophilus |
F159V | turnover number for hydantoin is 22% of that of the wild-type enzyme, Km-value for hydantoin is 1.6fold higher than that of the wild-type enzyme, turnover-number for DL-hydroxyphenylhydantoin is 2.8fold higher than that of the wild-type enzyme, Km-value for DL-hydroxyphenylhydantoin is 1.5fold lower than that of the wild-type enzyme | Geobacillus stearothermophilus |
L65F/F159A F159A | turnover number for hydantoin is 10.9% of that of the wild-type enzyme, Km-value for hydantoin is 4.8fold higher than that of the wild-type enzyme, turnover-number for DL-hydroxyphenylhydantoin is 11fold higher than that of the wild-type enzyme, Km-value for DL-hydroxyphenylhydantoin is 2.4fold higher than that of the wild-type enzyme. Specificity of the mutant enzyme towards aromatic substrate hydroxyphenylhydantoin is enhanced by 200fold compared with that of the wild-type enzyme | Geobacillus stearothermophilus |
M63F/L65V F159A | turnover number for hydantoin is 9.5% of that of the wild-type enzyme, Km-value for hydantoin is 2.6fold higher than that of the wild-type enzyme, turnover-number for DL-hydroxyphenylhydantoin is 10fold higher than that of the wild-type enzyme, Km-value for DL-hydroxyphenylhydantoin is 4.4fold higher than that of the wild-type enzyme. Specificity of the mutant enzyme towards aromatic substrate hydroxyphenylhydantoin is enhanced by 200fold compared with that of the wild-type enzyme | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
DL-hydroxyphenylhydantoin | pH 8.0, 37°C, mutant enzyme F159L | Geobacillus stearothermophilus | |
3.5 | - |
DL-hydroxyphenylhydantoin | pH 8.0, 37°C, mutant enzyme F159I | Geobacillus stearothermophilus | |
3.6 | - |
DL-hydroxyphenylhydantoin | pH 8.0, 37°C, mutant enzyme F159V | Geobacillus stearothermophilus | |
5.4 | - |
DL-hydroxyphenylhydantoin | pH 8.0, 37°C, wild-type enzyme | Geobacillus stearothermophilus | |
7.4 | - |
DL-hydroxyphenylhydantoin | pH 8.0, 37°C, mutant enzyme F159A | Geobacillus stearothermophilus | |
8 | 9 | hydantoin | pH 8.0, 37°C, mutant enzyme F159L | Geobacillus stearothermophilus | |
13 | - |
DL-hydroxyphenylhydantoin | pH 8.0, 37°C, mutant enzyme L65F/F159A | Geobacillus stearothermophilus | |
24 | - |
DL-hydroxyphenylhydantoin | pH 8.0, 37°C, mutant enzyme M63F/L65V | Geobacillus stearothermophilus | |
27 | - |
DL-hydroxyphenylhydantoin | pH 8.0, 37°C, mutant enzyme F159S | Geobacillus stearothermophilus | |
98 | - |
hydantoin | pH 8.0, 37°C, wild-type enzyme | Geobacillus stearothermophilus | |
160 | - |
hydantoin | pH 8.0, 37°C, mutant enzyme F159V | Geobacillus stearothermophilus | |
190 | - |
hydantoin | pH 8.0, 37°C, mutant enzyme F159I | Geobacillus stearothermophilus | |
260 | - |
hydantoin | pH 8.0, 37°C, mutant enzyme M63F/L65V | Geobacillus stearothermophilus | |
400 | - |
hydantoin | pH 8.0, 37°C, mutant enzyme F159S | Geobacillus stearothermophilus | |
450 | - |
hydantoin | pH 8.0, 37°C, mutant enzyme F159A | Geobacillus stearothermophilus | |
470 | - |
hydantoin | pH 8.0, 37°C, mutant enzyme L65F/F159A | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
SD1 | - |
Geobacillus stearothermophilus SD1 | - |
SD1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-hydroxymethylhydantoin + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
DL-hydroxymethylhydantoin + H2O | - |
Geobacillus stearothermophilus SD1 | ? | - |
? | |
DL-hydroxyphenylhydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-D-hydroxyphenylglycine | - |
? | |
DL-hydroxyphenylhydantoin + H2O | - |
Geobacillus stearothermophilus SD1 | N-carbamoyl-D-hydroxyphenylglycine | - |
? | |
DL-phenylhydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-D-phenylglycine | - |
? | |
DL-phenylhydantoin + H2O | - |
Geobacillus stearothermophilus SD1 | N-carbamoyl-D-phenylglycine | - |
? | |
hydantoin + H2O | - |
Geobacillus stearothermophilus | hydantoic acid | - |
? | |
hydantoin + H2O | - |
Geobacillus stearothermophilus SD1 | hydantoic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D-hydantoinase | - |
Geobacillus stearothermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
DL-hydroxyphenylhydantoin | pH 8.0, 37°C, mutant enzyme F159L | Geobacillus stearothermophilus | |
18 | - |
DL-hydroxyphenylhydantoin | pH 8.0, 37°C, wild-type enzyme | Geobacillus stearothermophilus | |
21 | - |
hydantoin | pH 8.0, 37°C, mutant enzyme F159S | Geobacillus stearothermophilus | |
42 | - |
hydantoin | pH 8.0, 37°C, mutant enzyme M63F/L65V | Geobacillus stearothermophilus | |
45 | - |
hydantoin | pH 8.0, 37°C, mutant enzyme F159A | Geobacillus stearothermophilus | |
46 | - |
DL-hydroxyphenylhydantoin | pH 8.0, 37°C, mutant enzyme F159I | Geobacillus stearothermophilus | |
48 | - |
hydantoin | pH 8.0, 37°C, mutant enzyme L65F/F159A | Geobacillus stearothermophilus | |
50 | - |
DL-hydroxyphenylhydantoin | pH 8.0, 37°C, mutant enzyme F159V | Geobacillus stearothermophilus | |
97 | - |
hydantoin | pH 8.0, 37°C, mutant enzyme F159V | Geobacillus stearothermophilus | |
110 | - |
DL-hydroxyphenylhydantoin | pH 8.0, 37°C, mutant enzyme F159A | Geobacillus stearothermophilus | |
180 | - |
DL-hydroxyphenylhydantoin | pH 8.0, 37°C, mutant enzyme M63F/L65V | Geobacillus stearothermophilus | |
200 | - |
DL-hydroxyphenylhydantoin | pH 8.0, 37°C, mutant enzyme L65F/F159A | Geobacillus stearothermophilus | |
270 | - |
hydantoin | pH 8.0, 37°C, mutant enzyme F159I | Geobacillus stearothermophilus | |
280 | - |
hydantoin | pH 8.0, 37°C, mutant enzyme F159L | Geobacillus stearothermophilus | |
280 | - |
DL-hydroxyphenylhydantoin | pH 8.0, 37°C, mutant enzyme F159S | Geobacillus stearothermophilus | |
440 | - |
hydantoin | pH 8.0, 37°C, wild-type enzyme | Geobacillus stearothermophilus |