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Literature summary for 3.5.2.14 extracted from

  • Ogawa, J.; Min Kim, J.; Nirdnoy, W.; Amano, Y.; Yamada, H.; Shimizu, S.
    Purification and characterization of an ATP-dependent amidohydrolase, N-methylhydantoin amidohydrolase, from Pseudomonas putida 77 (1995), Eur. J. Biochem., 229, 184-190.
No PubMed abstract available
Search for more literature for 3.5.2.14

Crystallization (Commentary)

Crystallization (Comment) Organism
addition of small portions of solid ammonium sulfate to the concentrated enzyme solution Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithio-bis(2-nitrobenzoic acid) 0.1 mM Pseudomonas putida
8-hydroxyquinoline 1 mM Pseudomonas putida
Ag2+ 1 mM Pseudomonas putida
Cd2+ 1 mM Pseudomonas putida
Cu2+ 1 mM Pseudomonas putida
Fe2+ 1 mM Pseudomonas putida
Hg2+ 0.1 mM Pseudomonas putida
hydroxylamine 1 mM Pseudomonas putida
N-Methylmaleimide 1 mM Pseudomonas putida
p-chloromercuribenzoate 0.1 mM Pseudomonas putida
phenylhydrazine 1 mM Pseudomonas putida
Semicarbazide 1 mM Pseudomonas putida
Zn2+ 1 mM Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
 N-methylhydantoin
-
 Pseudomonas putida
0.07
-
 glutarimide
-
 Pseudomonas putida
0.083
-
 dihydrouracil
-
 Pseudomonas putida
0.11
-
 delta-valerolactam
-
 Pseudomonas putida
0.13
-
 succimide
-
 Pseudomonas putida
0.75
-
 2-Pyrrolidone
-
 Pseudomonas putida
1.7
-
 hydantoin
-
 Pseudomonas putida
2
-
 Dihydrothymine
-
 Pseudomonas putida
2
-
 D,L-5-methylhydantoin
-
 Pseudomonas putida
6.6
-
 Uracil
-
 Pseudomonas putida
10
-
 2,4-Thiazolidinedione
-
 Pseudomonas putida
16
-
 thymine
-
 Pseudomonas putida
18
-
 2-Oxazolidone
-
 Pseudomonas putida
40
-
 2-Imidazolidone
-
 Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
K+ monovalent cations Pseudomonas putida
Mg2+ divalent cations Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 alpha2beta2 2 * 70000 + 2 * 80000, SDS-PAGE Pseudomonas putida
80000
-
 alpha2beta2 2 * 70000 + 2 * 80000, SDS-PAGE Pseudomonas putida
300000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + N-methylimidazolidine-2,4-dione + H2O Pseudomonas putida
-
 ADP + phosphate + N-carbamoylsarcosine
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
 77
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2,4-thiazolidinedione + H2O
-
 Pseudomonas putida ?
-
 ?
ATP + 2-imidazolidone + H2O
-
 Pseudomonas putida ?
-
 ?
ATP + 2-oxazolidone + H2O
-
 Pseudomonas putida ?
-
 ?
ATP + 2-pyrrolidone + H2O
-
 Pseudomonas putida ?
-
 ?
ATP + D,L-5-methylhydantoin + H2O
-
 Pseudomonas putida ?
-
 ?
ATP + delta-valerolactam + H2O
-
 Pseudomonas putida ?
-
 ?
ATP + dihydrothymine + H2O
-
 Pseudomonas putida ?
-
 ?
ATP + dihydrouracil + H2O
-
 Pseudomonas putida ?
-
 ?
ATP + glutarimide + H2O
-
 Pseudomonas putida ?
-
 ?
ATP + hydantoin + H2O
-
 Pseudomonas putida ?
-
 ?
ATP + N-methylimidazolidine-2,4-dione + H2O
-
 Pseudomonas putida ADP + phosphate + N-carbamoylsarcosine
-
 ?
ATP + succimide + H2O
-
 Pseudomonas putida ?
-
 ?
ATP + thymine + H2O
-
 Pseudomonas putida ?
-
 ?
ATP + uracil + H2O
-
 Pseudomonas putida ?
-
 ?

Subunits

Subunits Comment Organism
tetramer alpha2beta2 2 * 70000 + 2 * 80000, SDS-PAGE Pseudomonas putida

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50
-
 Pseudomonas putida

pH Range

pH Minimum pH Maximum Comment Organism
6 10
-
 Pseudomonas putida