Information on EC 3.5.2.14 - N-methylhydantoinase (ATP-hydrolysing)

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The expected taxonomic range for this enzyme is: Pseudomonas putida

EC NUMBER
COMMENTARY hide
3.5.2.14
-
RECOMMENDED NAME
GeneOntology No.
N-methylhydantoinase (ATP-hydrolysing)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + N-methylhydantoin + 2 H2O = ADP + phosphate + N-carbamoylsarcosine
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of amide bond
-
-
-
-
hydrolysis of phosphoric ester
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Arginine and proline metabolism
-
-
creatinine degradation II
-
-
Metabolic pathways
-
-
creatinine degradation
-
-
SYSTEMATIC NAME
IUBMB Comments
N-methylimidazolidine-2,4-dione amidohydrolase (ATP-hydrolysing)
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CAS REGISTRY NUMBER
COMMENTARY hide
100785-00-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 2,4-thiazolidinedione + H2O
?
show the reaction diagram
-
-
-
-
?
ATP + 2-imidazolidone + H2O
?
show the reaction diagram
-
-
-
-
?
ATP + 2-oxazolidone + H2O
?
show the reaction diagram
-
-
-
-
?
ATP + 2-pyrrolidone + H2O
?
show the reaction diagram
-
-
-
-
?
ATP + D,L-5-methylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
ATP + delta-valerolactam + H2O
?
show the reaction diagram
-
-
-
-
?
ATP + dihydrothymine + H2O
?
show the reaction diagram
-
-
-
-
?
ATP + dihydrouracil + H2O
?
show the reaction diagram
ATP + glutarimide + H2O
?
show the reaction diagram
-
-
-
-
?
ATP + hydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
ATP + N-methylimidazolidine-2,4-dione + H2O
ADP + phosphate + N-carbamoylsarcosine
show the reaction diagram
ATP + succimide + H2O
?
show the reaction diagram
-
-
-
-
?
ATP + thymine + H2O
?
show the reaction diagram
-
-
-
-
?
ATP + uracil + H2O
?
show the reaction diagram
-
-
-
-
?
CTP + dihydrouracil + H2O
?
show the reaction diagram
-
-
-
-
?
dATP + dihydrouracil + H2O
?
show the reaction diagram
-
-
-
-
?
dATP + N-methylimidazolidine-2,4-dione + H2O
dADP + phosphate + N-carbamoylsarcosine
show the reaction diagram
-
-
-
-
?
dGTP + dihydrouracil + H2O
?
show the reaction diagram
-
-
-
-
?
dITP + dihydrouracil + H2O
?
show the reaction diagram
-
-
-
-
?
dUTP + dihydrouracil + H2O
?
show the reaction diagram
-
-
-
-
?
GTP + dihydrouracil + H2O
?
show the reaction diagram
-
-
-
-
?
ITP + dihydrouracil + H2O
?
show the reaction diagram
-
-
-
-
?
UTP + dihydrouracil + H2O
?
show the reaction diagram
-
-
-
-
?
XTP + dihydrouracil + H2O
?
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + N-methylimidazolidine-2,4-dione + H2O
ADP + phosphate + N-carbamoylsarcosine
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5,5'-dithio-bis(2-nitrobenzoic acid)
-
0.1 mM
8-hydroxyquinoline
-
1 mM
Hg2+
-
0.1 mM
hydroxylamine
-
1 mM
N-methylhydantoin
-
inhibits the hydrolysis of other nucleoside triphosphates than ATP and dATP
N-Methylmaleimide
-
1 mM
p-chloromercuribenzoate
-
0.1 mM
phenylhydrazine
-
1 mM
Semicarbazide
-
1 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
10
2,4-Thiazolidinedione
-
-
40
2-Imidazolidone
-
-
18
2-Oxazolidone
-
-
0.75
2-Pyrrolidone
-
-
2
D,L-5-methylhydantoin
-
-
0.11
delta-valerolactam
-
-
2
Dihydrothymine
-
-
0.083
dihydrouracil
-
-
0.07
glutarimide
-
-
1.7
hydantoin
-
-
0.032
N-methylhydantoin
-
-
0.13
succimide
-
-
16
thymine
-
-
6.6
Uracil
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
-
ATP in presence of N-methylhydantoin
50
-
CTP in absence of N-methylhydantoin
55
-
CTP in presence of N-methylhydantoin
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37 - 70
-
CTP-hydrolysis
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70000
-
alpha2beta2 2 * 70000 + 2 * 80000, SDS-PAGE
80000
-
alpha2beta2 2 * 70000 + 2 * 80000, SDS-PAGE
300000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
-
alpha2beta2 2 * 70000 + 2 * 80000, SDS-PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
addition of small portions of solid ammonium sulfate to the concentrated enzyme solution
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
-
loss of 25% in activity
246618
10.6
-
loss of 30% in activity
246618
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
about 70% of initial activity remains
70
-
about 20% of initial activity remains
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
-
measurement of serum and urine creatinine
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