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Literature summary for 3.5.1.89 extracted from
- Catalysis by N-acetyl-D-glucosaminylphosphatidylinositol de-N-acetylase (PIG-L) from Entamoeba histolytica: new roles for conserved residues (2013), J. Biol. Chem., 288, 7590-7595.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of wild-type and mutant N-terminally MBP-tagged cytoplasmic catalytic domains in Escherichia coli strain TB1 | Entamoeba histolytica |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D102A | site-directed mutagenesis of the isolated cytoplasmic catalytic domain, the mutant is active in absence of metal ions, but well stimulated by metal ions | Entamoeba histolytica |
| D133A | site-directed mutagenesis of the isolated cytoplasmic catalytic domain, the mutant is active in absence of metal ions, but well stimulated by metal ions | Entamoeba histolytica |
| D45A | site-directed mutagenesis of the isolated cytoplasmic catalytic domain, the mutant is active in absence of metal ions, but well stimulated by metal ions | Entamoeba histolytica |
| D46A | site-directed mutagenesis of the isolated cytoplasmic catalytic domain, the mutant is only slightly active in absence of metal ions and not stimulated by metal ions | Entamoeba histolytica |
| D47A | site-directed mutagenesis of the isolated cytoplasmic catalytic domain, the mutant is only slightly active in absence of metal ions, but well stimulated by metal ions | Entamoeba histolytica |
| E79A | site-directed mutagenesis of the isolated cytoplasmic catalytic domain, the mutant is active in absence of metal ions, but well stimulated by metal ions | Entamoeba histolytica |
| H140A | site-directed mutagenesis of the isolated cytoplasmic catalytic domain, the mutant is only slightly active in absence of metal ions and not stimulated by metal ions | Entamoeba histolytica |
| H143A | site-directed mutagenesis of the isolated cytoplasmic catalytic domain, the mutant is only slightly active in absence of metal ions, but well stimulated by metal ions | Entamoeba histolytica |
| H43A | site-directed mutagenesis of the isolated cytoplasmic catalytic domain, the mutant is only slightly active in absence of metal ions, but well stimulated by metal ions | Entamoeba histolytica |
| additional information | comparison of secondary conformation of the mutant proteins, overview | Entamoeba histolytica |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00137 | - |
6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol | pH 5.5, 37°C, recombinant mutant H140A cytoplasmic catalytic domain, metal added | Entamoeba histolytica |  |
| 0.0016 | - |
6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol | pH 5.5, 37°C, recombinant mutant H140A cytoplasmic catalytic domain, no metal added | Entamoeba histolytica | |
| 0.00182 | - |
6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol | pH 5.5, 37°C, recombinant mutant D46A cytoplasmic catalytic domain, no metal added | Entamoeba histolytica | |
| 0.00195 | - |
6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol | pH 5.5, 37°C, recombinant mutant D46A cytoplasmic catalytic domain, metal added | Entamoeba histolytica | |
| 0.00195 | - |
6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol | pH 5.5, 37°C, recombinant wild-type cytoplasmic catalytic domain, no metal added | Entamoeba histolytica | |
| 0.00199 | - |
6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol | pH 5.5, 37°C, recombinant mutant H43A cytoplasmic catalytic domain, no metal added | Entamoeba histolytica | |
| 0.00209 | - |
6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol | pH 5.5, 37°C, recombinant wild-type cytoplasmic catalytic domain, metal added | Entamoeba histolytica | |
| 0.00263 | - |
6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol | pH 5.5, 37°C, recombinant mutant H43A cytoplasmic catalytic domain, metal added | Entamoeba histolytica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | stimulates | Entamoeba histolytica | |
| Mn2+ | stimulates, binding of Mn2+ to the isolated cytoplasmic catalytic domain of the enzyme results in a significant alteration in the global conformation of the protein | Entamoeba histolytica | |
| additional information | the enzyme exhibits a novel metal-independent albeit metal-stimulated activity | Entamoeba histolytica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O | Entamoeba histolytica | - |
6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Entamoeba histolytica | C4M0W5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminally wild-type and mutant MBP-tagged cytoplasmic catalytic domains from Escherichia coli strain TB1 by amylose affinity chromatography and removal of the tag, although the MBP tag does not significantly alter the activity of the enzyme | Entamoeba histolytica |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O = 6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + acetate | a general acid-base pair catalytic mechanism that involves enzyme residues Asp46 and His140 | Entamoeba histolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O | - |
Entamoeba histolytica | 6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + acetate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| N-acetyl-D-glucosaminylphosphatidylinositol de-N-acetylase | - |
Entamoeba histolytica |
| PIG-L | - |
Entamoeba histolytica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Entamoeba histolytica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
assay at | Entamoeba histolytica |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme functions at the second step of GPI anchor biosynthesis, converting N-acetylglucosaminylphosphatidylinositol to glucosaminylphosphatidylinositol. This step is conserved in the GPI biosynthesis pathway | Entamoeba histolytica |
| additional information | residues Asp46 and His140 of the enzyme are important for catalysis | Entamoeba histolytica |
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Release 2023.1 (January 2023)
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